FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493197
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATTACTGCTGCTCATATGCAGGTAGTCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG64830.43416910159878436No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC60980.40838549769387433No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA55140.3692747842381146No Hit
GTAATAAACAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG51160.34262056513641537No Hit
CTCCTGGACAGTCGATCATCATCTCCTGCACTGGAACCAGCAGTGATGTT43880.29386611411622177No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG43770.2931294397189386No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41800.27993627096759505No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCATCATCTCCTG40370.2703595038029141No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGACTACCTGCATATGAGCAGCAGTAATAAACAG39240.26279184863082367No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC38190.25575995665675727No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA34610.23178455354517855No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCAT32860.22006473358840126No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT31760.2126979896155698No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30760.2060009496402685No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT30590.20486245284446727No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30410.20365698564891302No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG29890.20017452486175638No Hit
GACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCATCATCT29140.19515174488028036No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29020.1943481000832442No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA28890.19347748488645503No Hit
GTCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC28370.18999502409929836No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28210.18892349770325015No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27760.18590982971436454No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC26600.17814126334301503No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG26320.17626609214993064No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26310.17619912175017766No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG26070.17459183215610533No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC25530.17097543056944262No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25350.1697699633738884No Hit
GTACTAGTGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGAAAGGCC25290.1693681409753703No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24970.16722508818327386No Hit
CCCCTGAGGGCCGCTTATTGTCCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA24790.16601962098771963No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA23920.1601931962092075No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC23360.15644285382303877No Hit
CCCAATGACTACCTGCATATGAGCAGCAGTAATAAACAGCCTCGTCCTCA23270.15584012022526164No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT22810.15275948183662302No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC22780.152558570637364No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGG22510.15075036984403262No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22410.1500806658465025No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22050.14766973145539403No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21990.14726790905687595No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21930.14686608665835788No Hit
TGTAAGGACTGTGTGTACAGTAATATGTGGCTGTGTCCGCAGGGTCCATG21240.14224512907539996No Hit
GCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCATCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGA21150.14164239547762286No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGAC20840.13956631308527945No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGACTTATAATTTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC20790.13923146108651438No Hit
GTCGATCATCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGACTTATAATTTTGTCTCCTGGTACCAACAGCA20410.13668658589589985No Hit
GCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCAT20410.13668658589589985No Hit
GGCAAAGCCCCCAAACTCATGATTTATGAGGACAATAAGCGGCCCTCAGG19610.13132895391565882No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG19130.1281143747275142No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC19050.1275786115294901No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC19030.12744467072998406No Hit
GCTTGGTCCCTCCGCCGAACACCCAATGACTACCTGCATATGAGCAGCAGTAATAAACAGCCTCGTCCTCAGC19020.12737770033023105No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18870.12637314433393584No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCG18550.12423009154183944No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC18290.12248886114826107No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGACTACCTGCATATGAGCAGCAGTAATAAACAGC18280.12242189074850805No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18280.12242189074850805No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18160.12161824595147191No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17410.11659546596999593No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGACTAC17360.11626061397123086No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTG17270.11565788037345373No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGTTTATTACTGCTGCTCATAT17270.11565788037345373No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC17070.11431847237839347No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGA16950.11351482758135732No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC16860.11291209398358021No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT16510.11056812999222472No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16420.10996539639444762No Hit
GTCCAGGAGACCCAGGCACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC16260.10889386999839942No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT16210.10855901799963433No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA16170.1082911364006223No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG16140.10809022520136326No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16080.10768840280284517No Hit
CAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGACAATAAGCGGCCCTCAGGGGTTTCTAATCG16010.10721961000457408No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15970.10695172840556202No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC15950.10681778760605601No Hit
GCTCCAGGCTGAGGACGAGGCTGTTTATTACTGCTGCTCATATGCAGGTAGTCATTGGGTGTTCGGCGGAGGGAC15730.10534443881148972No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15690.10507655721247765No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15460.10353623801815834No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC15180.10166106682507399No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG15170.10159409642532097No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15160.10152712602556795No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG15110.10119227402680289No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGACA15040.10072348122853181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2400.056.64590570
GTCGTAT200.00744095851.6060239
GTGGTAT2600.049.7572981
TGGTATC2750.048.2763942
GGTATCA10400.045.8290861
ACCGTAC2950.041.57503527
CGTACTA3050.041.36774429
GACCGTA3050.040.20383526
CGTATGC3100.040.08603341
TCGTATG3100.040.01807840
ACGAGAC3250.039.7991722
ACTAGAT3250.038.90034532
TATACAC3650.038.2366073
ATGCCGT3450.038.1942344
TCGCTAT558.113118E-637.13195411
TCTTATA1850.036.8011322
TACTAGA3650.036.48259731
CTTATAC3550.036.4419631
TTATACA3550.036.43832
TCTCGTA3000.035.51355438
Produced by FastQC (version 0.11.5)