FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493197
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATTACTGCTGCTCATATGCAGGTAGTCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG66910.4480989447474111No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC57820.38722285137192214No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA52310.3503221611080119No Hit
GTAATAAACAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG47630.3189800140236017No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45760.30645654926978827No Hit
CTCCTGGACAGTCGATCATCATCTCCTGCACTGGAACCAGCAGTGATGTT41170.27571713578315515No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38520.2579699798486067No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCATCATCTCCTG37230.24933079828046803No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36930.24732168628787762No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC34850.23339184313925088No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGACTACCTGCATATGAGCAGCAGTAATAAACAG34310.2297754415525882No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33550.22468569117135914No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCAT31930.21383648641137104No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT31550.21129161122075657No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA30340.20318819285064194No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29240.1958214488778105No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG28990.19414718888398516No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28050.18785197130720194No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG27900.18684741531090673No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT27840.18644559291238866No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27600.18483830331831633No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27380.18336495452375004No Hit
GTCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC27300.18282919132572595No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG26010.17419000975758725No Hit
GACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCATCATCT25550.17110937136894863No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25260.16916722977611126No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC25240.16903328897660524No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG25110.16816267377981606No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA25100.16809570338006305No Hit
CCCCTGAGGGCCGCTTATTGTCCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA24230.1622692786015509No Hit
GTACTAGTGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGAAAGGCC24000.1607289594072316No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC23800.15938955141217134No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC23730.15892075861390023No Hit
CCCAATGACTACCTGCATATGAGCAGCAGTAATAAACAGCCTCGTCCTCA22810.15275948183662302No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG22710.1520897778390929No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC22600.15135310344180974No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC22260.1490761098502073No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21990.14726790905687595No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21820.14612941226107473No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21150.14164239547762286No Hit
GCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCATCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGA21070.14110663227959874No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20890.13990116508404452No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20880.13983419468429148No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20750.13896357948750232No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGG20680.13849478668923124No Hit
GTCGATCATCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGACTTATAATTTTGTCTCCTGGTACCAACAGCA20350.1362847634973818No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC20050.1342756515047914No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19680.1317977467139299No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGACTTATAATTTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC19510.1306592499181287No Hit
GCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCAT19310.12931984192306842No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT19290.1291859011235624No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG19210.1286501379255383No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19080.12777952272874912No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGAC18860.1263061739341828No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCG18850.12623920353442983No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTG18750.12556949953689966No Hit
GCTTGGTCCCTCCGCCGAACACCCAATGACTACCTGCATATGAGCAGCAGTAATAAACAGCCTCGTCCTCAGC18620.1246988843401105No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC18230.122087038749743No Hit
GGCAAAGCCCCCAAACTCATGATTTATGAGGACAATAAGCGGCCCTCAGG18180.12175218675097793No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18140.12148430515196587No Hit
TGTAAGGACTGTGTGTACAGTAATATGTGGCTGTGTCCGCAGGGTCCATG17950.12021186755665862No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC17720.11867154836233934No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGACTAC17430.11672940676950194No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGTTTATTACTGCTGCTCATAT17430.11672940676950194No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC17150.11485423557641758No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGA16780.1123763307855561No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC16490.1104341891927187No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT16300.10916175159741145No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16100.10782234360235118No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT16040.10742052120383311No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15960.106884758005809No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15790.1057462612100078No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15770.10561232041050175No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG15660.10487564601321861No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGACTACCTGCATATGAGCAGCAGTAATAAACAGC15650.10480867561346562No Hit
GTTGGTACCAGGAGACAAAATTATAAGTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGATGATCGACTGTC15080.10099136282754385No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15020.10058954042902576No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTT200.00718964152.0564145
GTGGTAT4000.046.7844431
GGTATCA14100.042.7122961
TGGTATC4450.041.273612
TACTAGA400.002784225234.0125122
GTATCAA27150.033.2104531
CTAGCCA552.5844856E-432.6255261
TCTATTC751.8697519E-631.7365063
TATGCGG450.004966120230.2211975
GACAGGC2650.029.5311761
GTTGTAT950.005723714429.3540970
GGGTTAC704.2337975E-529.164331
ATAGCGG1252.570232E-928.86965263
CGTACCA854.962094E-627.99998714
TATCAAC32300.027.9050052
ATCAACG32400.027.7064743
GGATGGG9900.027.4638670
TCAACGC33050.027.261714
TAGGCAT500.0082498927.23829727
ACCGTCA500.008308087527.1990768
Produced by FastQC (version 0.11.5)