FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834439
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA31670.3795364310632653No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31570.3783380211135865No Hit
GTATTACTGCCAGCAGTATGGTAGTTCACCGTACACTTTTGGCCAGGGGA28820.3453817474974204No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27940.3348357399402473No Hit
GTGTATTACTGTGCGAAACAACTCTCAAGAGACACTGAAATTGCAGTGGT25650.3073921520926035No Hit
GTATGGTAGTTCACCGTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCAT23070.27647317539089133No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21900.2624517789796498No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20920.2507073614727979No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18010.2158336319371458No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17510.20984158218875196No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGTCAGACTTCA17490.2096019001988162No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGCCTGGATCCG16790.20121303055106485No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16550.19833684667183582No Hit
GTGTATTACTGCCAGCAGTATGGTAGTTCACCGTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAA16430.1968987547322213No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15510.18587338319517663No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAGCTCCTTAGCCTGGTACC15480.18551386021027302No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15440.1850344962304015No Hit
CTACCATACTGCTGGCAGTAATACACTGCAAAATCTTCAGGCTCCATTCTGTTGATGGTGAGAGTGAAGTCTGAC15410.1846749732454979No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14830.17772419553736102No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14690.17604642160781075No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCATTCTGTTGATGGTGAGAGTGAAGTCTGACCCAGACCCACTGCCA14560.17448848867322836No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14330.1717321457889672No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14060.16849643892483454No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG13810.16550041405063762No Hit
CTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCA13800.16538057305566972No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13640.16346311713618372No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGG13490.16166550221166556No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13060.15651233942804685No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12500.1498012437098458No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12450.1492020387350064No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11880.14237110202183742No Hit
GTATTACTGTGCGAAACAACTCTCAAGAGACACTGAAATTGCAGTGGTGGTAGGTGCTGCTCGAGTTGTGAGGG11870.14225126102686955No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11780.14117269207215866No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC11420.1368584162533151No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA11330.1357798472986042No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGA11310.13554016530866847No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT10910.1307465255099534No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG10860.13014732053511402No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC10840.12990763854517826No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCC10730.12858938760053162No Hit
GGCCAAAAGTGTACGGTGAACTACCATACTGCTGGCAGTAATACACTGCA10690.1281100236206601No Hit
GTAATACACTGCAAAATCTTCAGGCTCCATTCTGTTGATGGTGAGAGTGAAGTCTGACCCAGACCCACTGCCA10530.1261925677011741No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10420.12487431675652745No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10380.12439495277665592No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10360.12415527078672019No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10210.12235765586220203No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10160.12175845088736265No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10160.12175845088736265No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCCCCTGGCCAAAAGTG10080.12079972292761963No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9780.11720449307858333No Hit
GTCAGAGTGTTGGCAGCAGCTCCTTAGCCTGGTACCAGCGGAAACCTGGC9740.11672512909871183No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC9700.11624576511884033No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9530.11420846820438642No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9480.11360926322954704No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCCGCTGGTACCAGGCTAAGGAGCTGCTGCCAACACTCTGA9450.11324974024464342No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9220.11049339736038223No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9160.10977435139057498No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9140.10953466940063923No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9050.10845610044592834No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT9010.10797673646605684No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT8870.10629896253650656No Hit
ACCATACTGCTGGCAGTAATACACTGCAAAATCTTCAGGCTCCATTCTGT8840.10593943955160294No Hit
CCTTAGCCTGGTACCAGCGGAAACCTGGCCAGGCTCCCAGGCTCCTCATC8830.10581959855663506No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8830.10581959855663506No Hit
GACTTCACTCTCACCATCAACAGAATGGAGCCTGAAGATTTTGCAGTGTATTACTGCCAGCAGTATGGTAGTTCA8810.1055799165666993No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC8760.10498071159185993No Hit
GTAGTTCACCGTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGA8640.1035426196522454No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCAGTGTCCCTTGGCCCCAAATATCAAAAGCCCTCACAA8620.10330293766230964No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8550.1024640506975345No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8430.10102595875791998No Hit
GTGAACTACCATACTGCTGGCAGTAATACACTGCAAAATCTTCAGGCTCCATTCTGTTGATGGTGAGAGTGAAGT8350.10006723079817699No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCCG200.00685945152.67688452
GACGGAA8800.050.70535770
GGTATCA8900.044.0713581
CGTATGC1550.037.91037441
CGACGGA8300.037.3626469
TCGTATG1500.036.82048440
GCGACCT400.00221072935.64821260
GACCGTA1600.034.1544926
CTATATA501.9939346E-434.0971762
GTATCAA22800.033.808211
CACGAGA1650.033.0738421
ATCTCGT1500.032.10494637
ATCTCCG1700.032.0895410
ACGGGAC652.5137066E-531.47620616
GTACTAG1750.031.27953730
CGTACTA1750.031.26825729
TTATACA2200.030.9974352
TAGATCT1550.030.98126634
CTAGATC1550.030.9588733
TCGACGG9150.030.80298868
Produced by FastQC (version 0.11.5)