FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834439
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30260.3626388507727946No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA28670.34358413257290227No Hit
GTATTACTGCCAGCAGTATGGTAGTTCACCGTACACTTTTGGCCAGGGGA28440.3408277896886411No Hit
GTGTATTACTGTGCGAAACAACTCTCAAGAGACACTGAAATTGCAGTGGT27010.32369052740823473No Hit
GTATGGTAGTTCACCGTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCAT22770.27287794554185507No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21600.2588565491306135No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20500.24567403968414708No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20500.24567403968414708No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18520.22194552268050752No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17960.21523442696230638No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGCCTGGATCCG16580.19869636965673945No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16560.1984566876668037No Hit
GTGTATTACTGCCAGCAGTATGGTAGTTCACCGTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAA16040.1922249559284741No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15610.1870717931448554No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15070.18060037941659007No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGTCAGACTTCA14770.17700514956755375No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCATTCTGTTGATGGTGAGAGTGAAGTCTGACCCAGACCCACTGCCA14670.17580673961787502No Hit
CTACCATACTGCTGGCAGTAATACACTGCAAAATCTTCAGGCTCCATTCTGTTGATGGTGAGAGTGAAGTCTGAC14510.173889283698389No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14360.17209166877387083No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAGCTCCTTAGCCTGGTACC13790.16526073206070185No Hit
CTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCA13680.16394248111605522No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13480.16154566121669767No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13150.15759090838275777No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13030.15615281644314324No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12700.1521980636092033No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG12530.1501607666947494No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12300.14740442381048824No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12150.14560680888597008No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGG12050.14440839893629132No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11940.14309014799164468No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11780.14117269207215866No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11770.1410528510771908No Hit
GTATTACTGTGCGAAACAACTCTCAAGAGACACTGAAATTGCAGTGGTGGTAGGTGCTGCTCGAGTTGTGAGGG11760.14093301008222292No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11280.13518064232376484No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11170.13386239137911818No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA11120.1332631864042788No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10860.13014732053511402No Hit
GGCCAAAAGTGTACGGTGAACTACCATACTGCTGGCAGTAATACACTGCA10550.12643224969110983No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC10270.12307670183200929No Hit
GTAATACACTGCAAAATCTTCAGGCTCCATTCTGTTGATGGTGAGAGTGAAGTCTGACCCAGACCCACTGCCA10240.12271717884710567No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10090.12091956392258751No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCCCCTGGCCAAAAGTG9980.11960131297794087No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCC9810.11756401606348697No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGA9800.11744417506851909No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9670.1158862421339367No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCCGCTGGTACCAGGCTAAGGAGCTGCTGCCAACACTCTGA9640.11552671914903308No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9610.11516719616412943No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9590.11492751417419367No Hit
GTCAGAGTGTTGGCAGCAGCTCCTTAGCCTGGTACCAGCGGAAACCTGGC9420.11289021725973977No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9270.11109260233522164No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG9240.110733079350318No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9210.11037355636541438No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9200.1102537153704465No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9050.10845610044592834No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9040.10833625945096045No Hit
GACTTCACTCTCACCATCAACAGAATGGAGCCTGAAGATTTTGCAGTGTATTACTGCCAGCAGTATGGTAGTTCA9020.10809657746102472No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA8770.1051005525868278No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8620.10330293766230964No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8470.1015053227377915No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC8460.10138548174282362No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)