FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330239
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT50671.5343433089368608No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17520.5305248622967003No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13710.4151538734068356No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13200.39971051268929475No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC12990.3933514818056014No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12860.3894149388776008No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12720.38517558495513854No Hit
CCTATGAGCTGAGTCAGCCAACGTCAGTGTCCGTGTCCCCAGGACAGACA12470.3776053100935989No Hit
GCCTTGGGCTGACCTAGGACGATCAGCCTGGTCCCTCCGCCGAACACCAC11580.3506551315865177No Hit
CTTATGTGGTGTTCGGCGGAGGGACCAGGCTGATCGTCCTAGGTCAGCCC10140.3070503483840491No Hit
CCACATAAGTGCTGTCCCACGTCTGACAGTAATAGTCAGCCTCATCCGTA10130.30674753738958754No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC9950.301296939489279No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9870.29887445153358627No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9820.29736039656127833No Hit
CCCCTGTTCTGGTCATCTATCAAGATGACATACGACCTTCAGGGATCCCT9800.29675477457235516No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9670.29281823164435455No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGCCTGGTCCCTC9450.28615638976619967No Hit
GGATTCGTGGCCTCCTATGAGCTGAGTCAGCCAACGTCAGTGTCCGTGTCCCCAGGACAGACAGCCACCATCCC8490.2570865342978873No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8480.2567837233034257No Hit
GAACAGGGGACTGGCCTGGCCTCTGTTGATACCAAGAAACGTATTTCTCC8180.24769939346957812No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG8100.2452769055138854No Hit
GACTATTACTGTCAGACGTGGGACAGCACTTATGTGGTGTTCGGCGGAGGGACCAGGCTGATCGTCCTAGGTCAG8090.2449740945194238No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT8080.24467128352496223No Hit
GGCCAGGCCAGTCCCCTGTTCTGGTCATCTATCAAGATGACATACGACCT7980.24164317358034634No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC7780.23558695369111463No Hit
CTATTACTGTCAGACGTGGGACAGCACTTATGTGGTGTTCGGCGGAGGGACCAGGCTGATCGTCCTAGGTCAGCC7710.2334672767298835No Hit
CATAAGTGCTGTCCCACGTCTGACAGTAATAGTCAGCCTCATCCGTAGCCCGGGTCCCGCTGATGGTCAGAGTGG7540.22831948982403655No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7440.22529137987942066No Hit
GCACTTATGTGGTGTTCGGCGGAGGGACCAGGCTGATCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT7440.22529137987942066No Hit
GTAATAGTCAGCCTCATCCGTAGCCCGGGTCCCGCTGATGGTCAGAGTGG7340.22226326993480477No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG7060.21378456208988034No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7050.21348175109541875No Hit
GACCTAGGACGATCAGCCTGGTCCCTCCGCCGAACACCACATAAGTGCTGTCCCACGTCTGACAGTAATAGTCAG6770.20500304325049434No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG6730.20379179927264798No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACCGTCACGGCTC6650.20136931131695532No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC6650.20136931131695532No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6600.19985525634464738No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG6550.19834120137233943No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6460.19561590242218513No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG6140.18592595059941436No Hit
GTATCAACAGAGGCCAGGCCAGTCCCCTGTTCTGGTCATCTATCAAGATG5920.17926410872125945No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACGGTGGC5730.1735106998264893No Hit
GCTATCTGCCTTCCAGGCCACCGTCACGGCTCCCGGGTAGAAGTCACTTA5680.17199664485418137No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA5640.17078540087633504No Hit
GTTCTGGTCATCTATCAAGATGACATACGACCTTCAGGGATCCCTGAGCG5470.16563761397048804No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA5460.16533480297602646No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG5450.16503199198156487No Hit
CTCTGGGACCGCAGCCACTCTGACCATCAGCGGGACCCGGGCTACGGATG5360.16230669303141057No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC5250.15897577209233313No Hit
CAGTAATAGTCAGCCTCATCCGTAGCCCGGGTCCCGCTGATGGTCAGAGT5250.15897577209233313No Hit
CCCCAGGACAGACAGCCACCATCCCCTGTTCTGGAGATAAATTGGGGGAG5210.15776452811448677No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC5120.1550392291643325No Hit
GGACAGCACTTATGTGGTGTTCGGCGGAGGGACCAGGCTGATCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT5070.15352517419202455No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACGGTGGCCTGGA5070.15352517419202455No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5000.15140549723079347No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG4910.14868019828063916No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT4870.14746895430279283No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG4810.1456520883360233No Hit
GGCCAGTCCCCTGTTCTGGTCATCTATCAAGATGACATACGACCTTCAGG4790.14504646634710014No Hit
GTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAAGGTCGTAT4720.14292678938586903No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC4410.13353964855755984No Hit
GAGTACGGGCTGGGCCTCAGGAAGCAGCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGT4320.13081434960740554No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCA4290.1299059166240208No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACCGTCA4250.12869467264617446No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG4250.12869467264617446No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG4220.12778623966278968No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATTCGTGG4200.12718061767386651No Hit
CTGTTGATACCAAGAAACGTATTTCTCCCCCAATTTATCTCCAGAACAGGGGATGGTGGCTGTCTGTCCTGGGGA4180.12657499568494332No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGCCTGGTCCCTCCGCCGAACACCACATAAGTG4180.12657499568494332No Hit
GGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA4150.12566656270155857No Hit
GGGCTGCTATCTGCCTTCCAGGCCACCGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC4130.12506094071263538No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC4090.12384969673478906No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG4080.12354688574032746No Hit
GAGTGGCTGCGGTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT4070.12324407474586588No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC4030.12203283076801952No Hit
GTGCTGTCCCACGTCTGACAGTAATAGTCAGCCTCATCCGTAGCCCGGGT3990.12082158679017317No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGC3980.12051877579571159No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT3890.11779347684555731No Hit
ACATACGACCTTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGG3820.11567379988432619No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACGGTGGCCTGGAAGGCAGATAGCAGCC3800.11506817789540304No Hit
GGACCAGGCTGATCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG3800.11506817789540304No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC3790.11476536690094145No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATTCGTGGCCTCCTATGAGCTGAGT3720.11264568993971033No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT3700.11204006795078715No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT3700.11204006795078715No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG3690.11173725695632557No Hit
GGCCTGGCCTCTGTTGATACCAAGAAACGTATTTCTCCCCCAATTTATCTCCAGAACAGGGGATGGTGGCTGT3680.11143444596186397No Hit
GTTCTGGAGATAAATTGGGGGAGAAATACGTTTCTTGGTATCAACAGAGG3640.11022320198401765No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA3620.10961757999509447No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG3600.10901195800617129No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT3540.10719509203940177No Hit
CAGCCACTCTGACCATCAGCGGGACCCGGGCTACGGATGAGGCTGACTAT3530.10689228104494017No Hit
CCCCTGTTCTGGAGATAAATTGGGGGAGAAATACGTTTCTTGGTATCAACAGAGGCCAGGCCAGTCCCCTGTT3510.106286659056017No Hit
GCCTGGTCCCTCCGCCGAACACCACATAAGTGCTGTCCCACGTCTGACAG3490.10568103706709384No Hit
GTATCAACGCAGAGTACGGGCTGGGCCTCAGGAAGCAGCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCT3380.10235011612801637No Hit
CTTCTACCCGGGAGCCGTGACGGTGGCCTGGAAGGCAGATAGCAGCCCCG3370.10204730513355478No Hit
ACCTTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGACCGCAG3370.10204730513355478No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG3330.10083606115570845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4450.094.3694270
CTATAAA200.00868719149.614823
CTAACGC200.00868719149.614823
ATGTCGT507.235394E-848.0308637
CTTGAAA8800.046.708957
ATGCCGT8300.046.6176344
CGTCTTC8350.046.5392548
CGTATGC8450.045.79342341
TGCTTGA8650.045.53869255
TCTCGTA7350.045.46255538
GTATGCC8550.045.45368242
GCCGTCT8500.045.41406646
TATGCCG8500.045.40376343
CCGTCTT8600.045.4008547
GTAATCT7400.044.1124534
ACAGGTA608.103598E-944.1087381
CGCGTAG8500.044.0973728
AATCTCG7450.044.0702236
CTCGTAT7700.044.0030639
TAATCTC7450.043.95323635
Produced by FastQC (version 0.11.5)