FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330239
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85462.5878227586687217No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16230.4914622440111555No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14470.4381675089859162No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14050.4254494472185296No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12990.3933514818056014No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11230.34005674678036213No Hit
CCTATGAGCTGAGTCAGCCAACGTCAGTGTCCGTGTCCCCAGGACAGACA10810.32733868501297547No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10720.32461338606282114No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10600.32097965412928214No Hit
GCCTTGGGCTGACCTAGGACGATCAGCCTGGTCCCTCCGCCGAACACCAC10510.3182543551791278No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10060.30462786042835643No Hit
CTTATGTGGTGTTCGGCGGAGGGACCAGGCTGATCGTCCTAGGTCAGCCC9860.29857164053912466No Hit
CCCCTGTTCTGGTCATCTATCAAGATGACATACGACCTTCAGGGATCCCT9490.28736763374404595No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9080.2749523829711209No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG9040.27374113899327457No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGCCTGGTCCCTC8950.2710158400431203No Hit
CCACATAAGTGCTGTCCCACGTCTGACAGTAATAGTCAGCCTCATCCGTA8770.26556524214281174No Hit
GGATTCGTGGCCTCCTATGAGCTGAGTCAGCCAACGTCAGTGTCCGTGTCCCCAGGACAGACAGCCACCATCCC8220.24891063744742448No Hit
GACTATTACTGTCAGACGTGGGACAGCACTTATGTGGTGTTCGGCGGAGGGACCAGGCTGATCGTCCTAGGTCAG8060.24406566153603904No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC7850.23770663065234574No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7840.23740381965788415No Hit
GAACAGGGGACTGGCCTGGCCTCTGTTGATACCAAGAAACGTATTTCTCC7770.23528414269665304No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7670.23225603275203716No Hit
GGCCAGGCCAGTCCCCTGTTCTGGTCATCTATCAAGATGACATACGACCT7600.23013635579080607No Hit
CTATTACTGTCAGACGTGGGACAGCACTTATGTGGTGTTCGGCGGAGGGACCAGGCTGATCGTCCTAGGTCAGCC7520.22771386783511338No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT7300.22105202595695841No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7250.21953797098465053No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7150.21650986104003464No Hit
GCACTTATGTGGTGTTCGGCGGAGGGACCAGGCTGATCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT7150.21650986104003464No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7090.2146929950732651No Hit
GTAATAGTCAGCCTCATCCGTAGCCCGGGTCCCGCTGATGGTCAGAGTGG6970.2110592631397261No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG6780.20530585424495593No Hit
CATAAGTGCTGTCCCACGTCTGACAGTAATAGTCAGCCTCATCCGTAGCCCGGGTCCCGCTGATGGTCAGAGTGG6640.20106650032249374No Hit
GACCTAGGACGATCAGCCTGGTCCCTCCGCCGAACACCACATAAGTGCTGTCCCACGTCTGACAGTAATAGTCAG6520.19743276838895465No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACCGTCACGGCTC6480.19622152441110832No Hit
CTCTGGGACCGCAGCCACTCTGACCATCAGCGGGACCCGGGCTACGGATG6220.18834843855510705No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG5940.17986973071018264No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT5930.17956691971572103No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG5930.17956691971572103No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACGGTGGCCTGGA5690.17229945584864295No Hit
GTTCTGGTCATCTATCAAGATGACATACGACCTTCAGGGATCCCTGAGCG5530.16745447993725757No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG5530.16745447993725757No Hit
GCTATCTGCCTTCCAGGCCACCGTCACGGCTCCCGGGTAGAAGTCACTTA5510.16684885794833437No Hit
GTATCAACAGAGGCCAGGCCAGTCCCCTGTTCTGGTCATCTATCAAGATG5490.16624323595941123No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA5440.1647291809871033No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5350.16200388203694902No Hit
GGACAGCACTTATGTGGTGTTCGGCGGAGGGACCAGGCTGATCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT5340.16170107104248743No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACGGTGGC5280.15988420507571788No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA5270.1595813940812563No Hit
GTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAAGGTCGTAT5240.15867296109787155No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC5110.1547364181698709No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT5090.15413079618094774No Hit
GGCCAGTCCCCTGTTCTGGTCATCTATCAAGATGACATACGACCTTCAGG4920.14898300927510075No Hit
CCCCAGGACAGACAGCCACCATCCCCTGTTCTGGAGATAAATTGGGGGAG4770.14444084435817695No Hit
GGGCTGCTATCTGCCTTCCAGGCCACCGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC4670.1414127344135611No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC4640.14050430143017634No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATTCGTGG4480.13565932551879092No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC4430.13414527054648298No Hit
GAGTACGGGCTGGGCCTCAGGAAGCAGCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGT4420.13384245955202143No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG4370.13232840457971348No Hit
CAGTAATAGTCAGCCTCATCCGTAGCCCGGGTCCCGCTGATGGTCAGAGT4290.1299059166240208No Hit
GGCCTGGCCTCTGTTGATACCAAGAAACGTATTTCTCCCCCAATTTATCTCCAGAACAGGGGATGGTGGCTGT4220.12778623966278968No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG4200.12718061767386651No Hit
GGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA4180.12657499568494332No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC4110.12445531872371224No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG4100.12415250772925064No Hit
GAGTGGCTGCGGTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT4020.12173001977355793No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG4010.12142720877909635No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT4000.12112439778463475No Hit
GGACCAGGCTGATCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG3980.12051877579571159No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGCCTGGTCCCTCCGCCGAACACCACATAAGTG3980.12051877579571159No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG3900.1180962878400189No Hit
GTGCTGTCCCACGTCTGACAGTAATAGTCAGCCTCATCCGTAGCCCGGGT3880.11749066585109572No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGC3790.11476536690094145No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC3760.11385693391755669No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATTCGTGGCCTCCTATGAGCTGAGT3750.1135541229230951No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG3710.11234287894524875No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACCGTCA3670.11113163496740239No Hit
CCCCTGTTCTGGAGATAAATTGGGGGAGAAATACGTTTCTTGGTATCAACAGAGGCCAGGCCAGTCCCCTGTT3670.11113163496740239No Hit
CTGTTGATACCAAGAAACGTATTTCTCCCCCAATTTATCTCCAGAACAGGGGATGGTGGCTGTCTGTCCTGGGGA3640.11022320198401765No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT3640.11022320198401765No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG3580.10840633601724811No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC3560.10780071402832495No Hit
GTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCT3550.10749790303386335No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG3540.10719509203940177No Hit
ACATACGACCTTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGG3530.10689228104494017No Hit
GTATCAACGCAGAGTACGGGCTGGGCCTCAGGAAGCAGCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCT3470.10507541507817067No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC3450.10446979308924749No Hit
CTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTG3420.10356136010586273No Hit
GCCTGGTCCCTCCGCCGAACACCACATAAGTGCTGTCCCACGTCTGACAG3380.10235011612801637No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC3340.10113887215017002No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCA3330.10083606115570845No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT3320.10053325016124687No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGCG150.00273609666.4315820
ACGCTAC200.006254471453.9129652
GCGGTAT200.00849302549.89938424
ACGCCAT200.00860064149.740696
TTACCTG200.00860064149.740695
CTAACGC200.0086057949.7331543
TGAGTAC452.4394176E-644.2139474
GATTGAC505.0231065E-639.8046041
CTTGAGT505.0388153E-639.7865222
GCTTGAG603.9498082E-738.698921
TGGGTAG750.001851016436.94333370
GGAGTAC451.263661E-436.8393753
TAACGCC400.00314932933.1604584
ACTTCCC450.003481811832.48702657
ATCAATA450.004108777731.40709747
GCTTGCC752.2618278E-630.9591391
TGCTACC500.004430296430.92634267
ACTGTAA450.00477784530.45372837
CAACTGG450.005112254530.0354631
ACAGGTA1002.1156666E-829.8534531
Produced by FastQC (version 0.11.5)