FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711382
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26500.37251434531658095No Hit
GTGATATACAGCCGTGTCCTCAGGTCTCAGGTTGTTCATTTCCAGATACA26300.36970291629532376No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24960.3508663418529004No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA19590.2753794726321442No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17640.24796803967488634No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT16690.23461375182391458No Hit
CTCCTGTACAGCCTCTGGATTCACCTTCAGTAACTATGGCATGGAGTGGATCCGCCAGGCTCCAGGCAAGGGG16030.22533603605376576No Hit
GTATATCACTGTGCGAGAGAAAAGGACGTATTTATCAGTTCGGCCATTGA15800.22210289267931999No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15610.21943203510912562No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15400.21648003463680554No Hit
CTCCTGCAGGGCCAGTCAGACTATTAGCAGAAACTTAGCCTGGTACCAACAGAAACCTGGCCAGACTCCCAGGCT15300.21507432012617694No Hit
GTCTTTGTCTGCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGCAGAAACTTAGCCTGGTA14990.21071660514322826No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14730.20706174741559388No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14690.20649946161134242No Hit
GTCTCCAGCCACCCTGTCTTTGTCTGCAGGGGAAAGAGCCACCCTCTCCT14610.20537489000283954No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14190.1994708890581994No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13510.18991203038592486No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13290.18681945846254194No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12750.17922860010514743No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTTACCAGCGGACCAGCTGGCCGGCCACCTTCGGCCAAGGGA12560.1765577425349531No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT12520.17599545673070163No Hit
GTTTATTACTGTTACCAGCGGACCAGCTGGCCGGCCACCTTCGGCCAAGGGACACGACTAGAGATTAAACGAA11710.16460916919460994No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11610.1632034546839813No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTTACCAGCGGACCAGCTGG11370.15982973985847268No Hit
CTCCCAGGCTCCTCATCTATGAGATTTCCAACAGGGCCACTGGCATCCCA11320.15912688260315838No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11080.1557531677776497No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10930.1536445960117068No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10630.14942745247982098No Hit
GTGCAGCCACAGTTCGTTTAATCTCTAGTCGTGTCCCTTGGCCGAAGGTG10580.14872459522450665No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10350.14549145185006085No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10120.14225830847561508No Hit
ATACTATGTAGACTCCGTGCAGGGCCGATTCACCATCTCCAGAGACAATT9830.13818173639479212No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9620.13522973592247203No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCTAGTCGTGTCCCTTGGCCGAAGGTGGCCGGCCAG9540.13410516431396916No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9480.13326173560759197No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9450.1328400212544034No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9360.13157487819483765No Hit
CGACTAGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT9330.13115316384164907No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9150.1286228777225176No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA8930.12553030579913466No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA8890.1249680199948832No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC8850.12440573419063175No Hit
ATGTAGACTCCGTGCAGGGCCGATTCACCATCTCCAGAGACAATTCCAGG8840.1242651627395689No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8780.12342173403319173No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8770.12328116258212887No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA8530.11990744775662021No Hit
ACATAGTATTGCGCCCCTCCATTATGTGATATGACCGCCACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGATC8310.11681487583323727No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT8230.1156903042247344No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG8190.11512801842048295No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8160.11470630406729436No Hit
ACAGAAACCTGGCCAGACTCCCAGGCTCCTCATCTATGAGATTTCCAACAGGGCCACTGGCATCCCAGCCAGGTT8050.1131600181056029No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC7850.1103485890843457No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7780.10936458892690565No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7750.10894287457371707No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7700.10824001731840277No Hit
TTATTACTGTTACCAGCGGACCAGCTGGCCGGCCACCTTCGGCCAAGGGA7650.10753716006308847No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7520.10570973119927128No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7420.10430401668864266No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7260.10205487347163689No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7260.10205487347163689No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGCAGAAACTTAGCCTGGTACCAACAGAAACCTGGCCAGA7140.10036801605888257No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTA252.5760202E-455.15866537
ATGCGTT252.5958987E-455.07290636
ACTACGC200.00697117552.4613351
ATCTCGT1200.048.838437
CGCAATT305.556564E-447.20172560
TCTGGAG7550.046.8981470
GGTATCA6950.045.3204961
TCGTATG1300.045.1935640
CGTATGC1300.042.5895141
ACGCAAT350.001199760540.3705959
CTTATAC1500.038.8015671
GTCGTAA459.4711344E-538.696348
CCCACGA1600.038.5189719
TATACAC1600.038.5162663
ATGCCGT1450.038.26253544
ACGAGAC1550.037.5683922
ATCTCCG1550.037.54726410
CGAGACA1600.036.3943823
CGAGCCC1600.036.37647215
TCTCCGA1600.036.37391311
Produced by FastQC (version 0.11.5)