FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711382
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26780.3764503459463411No Hit
GTGATATACAGCCGTGTCCTCAGGTCTCAGGTTGTTCATTTCCAGATACA25780.362393200840055No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20850.2930914754660647No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18950.2663828997641211No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17740.24937375418551494No Hit
CTCCTGTACAGCCTCTGGATTCACCTTCAGTAACTATGGCATGGAGTGGATCCGCCAGGCTCCAGGCAAGGGG16750.23545718053029174No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT16360.22997489393884016No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16290.22899089378140017No Hit
GTATATCACTGTGCGAGAGAAAAGGACGTATTTATCAGTTCGGCCATTGA15110.21240346255598255No Hit
CTCCTGCAGGGCCAGTCAGACTATTAGCAGAAACTTAGCCTGGTACCAACAGAAACCTGGCCAGACTCCCAGGCT14750.20734289031771957No Hit
GTCTTTGTCTGCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGCAGAAACTTAGCCTGGTA14450.20312574678583378No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14320.20129831792201658No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13640.19173945924974206No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13440.18892803022848484No Hit
GTCTCCAGCCACCCTGTCTTTGTCTGCAGGGGAAAGAGCCACCCTCTCCT13310.18710060136466763No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13040.1833051721859704No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT12690.17838517139877028No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12630.17754174269239312No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTTACCAGCGGACCAGCTGGCCGGCCACCTTCGGCCAAGGGA12030.16910745562862148No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11840.16643659805842712No Hit
CTCCCAGGCTCCTCATCTATGAGATTTCCAACAGGGCCACTGGCATCCCA11780.16559316935204996No Hit
GTTTATTACTGTTACCAGCGGACCAGCTGGCCGGCCACCTTCGGCCAAGGGACACGACTAGAGATTAAACGAA11740.1650308835477985No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11220.15772116809252976No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTTACCAGCGGACCAGCTGG11050.1553314534244611No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10950.15392573891383252No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10950.15392573891383252No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10560.14844345232238096No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10520.1478811665181295No Hit
GTGCAGCCACAGTTCGTTTAATCTCTAGTCGTGTCCCTTGGCCGAAGGTG10490.1474594521649409No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10470.1471783092628152No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10440.14675659490962661No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10200.14338288008411798No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC10180.14310173718199223No Hit
ATACTATGTAGACTCCGTGCAGGGCCGATTCACCATCTCCAGAGACAATT9940.13972802235648357No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCTAGTCGTGTCCCTTGGCCGAAGGTGGCCGGCCAG9630.1353703073735349No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9430.13255887835227767No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8930.12553030579913466No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA8840.1242651627395689No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT8830.12412459128850603No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA8740.12285944822894028No Hit
CGACTAGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT8520.11976687630555734No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGCTGACACAGTC8430.1185017332459916No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8410.1182205903438659No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8270.11625259002898583No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT8190.11512801842048295No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA8160.11470630406729436No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8120.11414401826304293No Hit
TTATTACTGTTACCAGCGGACCAGCTGGCCGGCCACCTTCGGCCAAGGGA7810.10978630328009424No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7800.10964573182903137No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG7700.10824001731840277No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7590.1066937313567113No Hit
ACAGAAACCTGGCCAGACTCCCAGGCTCCTCATCTATGAGATTTCCAACAGGGCCACTGGCATCCCAGCCAGGTT7390.10388230233545409No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7340.10317944508013979No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7330.10303887362907692No Hit
ACATAGTATTGCGCCCCTCCATTATGTGATATGACCGCCACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGATC7300.10261715927588834No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC7240.10177373056951117No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7190.10107087331419687No Hit
ATGTAGACTCCGTGCAGGGCCGATTCACCATCTCCAGAGACAATTCCAGG7130.1002274446078197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)