FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1624234
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA53260.32790841713693963No Hit
GTATAATAACTGGCCTCCGTTCACTTTTGGCCAGGGGACCAAGCTGCAGATCAAACGAACTGTGGCTGCACCAT42600.26227747972274934No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG37430.23044709075170203No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36570.22515228717044466No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33150.2040962078124211No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT28550.17577516540104443No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT28340.17448224824748157No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27880.1716501440063439No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26830.16518555823852968No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA26690.16432361346948776No Hit
ATATATTACTGTGCGAAGTCGGTTGGGGGTTCGGGGAGTCCACATTACTACTTTGACTACTGGGGCCAGGGAGCC26480.16303069631592493No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24080.14825450027520665No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT23270.14326753411146423No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22190.136618245893141No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA21220.13064619999335073No Hit
GAGTATTAGCAGCGACTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATC20970.1291070129057759No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20940.12892231045526692No Hit
CAGTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA20900.12867604052125495No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20870.12849133807074598No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20560.1265827460821532No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT20420.12572080131311128No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20190.12430474919254246No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19960.12288869707197361No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG19670.12110324005038682No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19260.11857897322676413No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT19150.11790173090823121No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC18590.11445395183206361No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT18380.11316103467850075No Hit
CTCCTGTGCAGCCTCTGGGTTCATTTTCAGTACCTATACCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG18260.11242222487646485No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17860.10995952553634514No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17500.1077430961302374No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17310.10657331394368054No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17290.10645017897667455No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC17180.10577293665814161No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT16990.10460315447158475No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCGACTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT16870.10386434466954884No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT16570.10201732016445907No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT16450.10127851036242316No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAG16280.10023186314287227No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG352.5037025E-548.487532
CTAACGC352.5037025E-548.487533
ACTACGC4500.041.5464527
CTACGCT4650.040.96632428
ACGAGAC4900.039.48999822
CTTATAC5200.037.8622321
TACGCTG4950.037.82315429
GGTATCA18150.037.5924841
CGAGCCC5250.036.8459815
CACGAGA5300.036.50961721
TCTGGAG19400.036.0924270
CGTATGC2500.035.9159741
CGAGACT5350.034.90686823
TCGCGTA807.367271E-834.35378338
ATCGCGT1004.0563464E-1034.30140337
TCGTATG2250.033.71434440
GTATCAA41200.033.6158831
TTATACA6150.032.5615432
ATGCCGT3100.032.47543344
ATCTCGT2750.032.4304237
Produced by FastQC (version 0.11.5)