FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1092891
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCCTCGGCTGTCACGCTGCTCATCTGCAGATACACTGAGTCTTTGGTGTTGTCTCTGGA41450.3792692958401158No Hit
GTTTATTACTGTGCGAGAGGGCCCGAATGCATTAATGGTATCTGTCATTT39650.3627992178542966No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36370.33278707574680366No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30880.28255333789005493No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC25210.23067259223472422No Hit
GTATGATGACTGGCCTCGAGCGTTCGGCCAAGGGACCAGGGTGGAGGTCAAACGAACTGTGGCTGCACCAT24450.22371855930737833No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22430.20523547178995893No Hit
GTCATCATACTGCTGACAGTAATAAACTGCAGAATCTTCAGGCTGCACGC21220.1941639193661582No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21150.19352341633337636No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20080.18373286997513935No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19950.18254336434283014No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19320.17677883704779343No Hit
GAGCTGGGACTGTGCTGGGTTTTCCTTGTCGCTATTTTAGAAGGTGTCCAATGTGAGATACAACTTGTGGAGT19110.17485732794944783No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18980.17366782231713868No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18720.17128881105252033No Hit
GTGCTGGGTTTTCCTTGTCGCTATTTTAGAAGGTGTCCAATGTGAGATAC18670.17083130888624756No Hit
CTCCTGTGCAGCCTCTGGATTCACATTTAGGGATTTTGCGATGAACTGGG18260.16707979112281096No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18230.1668052898230473No Hit
CTCCTGCAGGGCCAGTGCGAGTATTAGGAGCAATTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT18170.16625628722352No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGGGCCACCCTCTCCTGCAGGGCCAGTGCGAGTATTAGGAG18000.16470077985819262No Hit
CGTCTATGCTGCATCCACCGGGACCACTGGTCTGGCATCAAGGTTCAGTGGCAGTGGGTCTGGAACAGAGTTCAC17870.16351127422588346No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17550.1605832603617378No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17340.15866175126339224No Hit
GTTTATTACTGTCAGCAGTATGATGACTGGCCTCGAGCGTTCGGCCAAGGGACCAGGGTGGAGGTCAAACGAA17300.15829574953037404No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17210.15747224563108306No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16890.15454423176693743No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16730.15308022483486458No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16640.15225672093557363No Hit
GAGTTCACCCTCACCATCACCAGCGTGCAGCCTGAAGATTCTGCAGTTTATTACTGTCAGCAGTATGATGACTGG16530.15125021616977358No Hit
GTCTGGAACAGAGTTCACCCTCACCATCACCAGCGTGCAGCCTGAAGATT16190.14813920143911882No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16100.14731569753982784No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16010.1464921936405369No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15870.14521118757497317No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15780.1443876836756822No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15630.14301517717686393No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15450.141368169378282No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15380.14072766634550016No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15200.1390806585469182No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15170.1388061572471546No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14710.1345971373174452No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGCTGGGACTGTGCTGGG14600.13359063255164513No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC14520.13285862908560872No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14420.1319436247530632No Hit
GTCCAGAGACAACACCAAAGACTCAGTGTATCTGCAGATGAGCAGCGTGACAGCCGAGGACACGGCTGTTTATTA14420.1319436247530632No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13860.12681960049080832No Hit
GTCTGTGTCTCCAGGGGAAAGGGCCACCCTCTCCTGCAGGGCCAGTGCGAGTATTAGGAGCAATTTAGCCTGGTA13330.1219700775283171No Hit
GTAATAAACTGCAGAATCTTCAGGCTGCACGCTGGTGATGGTGAGGGTGAACTCTGTTCCAGACCCACTGCCA13330.1219700775283171No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13260.12132957449553522No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13230.12105507319577159No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12800.11712055456582586No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12610.11538204633398938No Hit
CACCATCACCAGCGTGCAGCCTGAAGATTCTGCAGTTTATTACTGTCAGCAGTATGATGACTGGCCTCGAGCGTT12400.11346053723564381No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12300.11254553290309831No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGCGACCAGGGTTCCCTGGCCCCAAAAGTCGAACCAGCGTGGCG12290.11245403246984374No Hit
GTTCCAGACCCACTGCCACTGAACCTTGATGCCAGACCAGTGGTCCCGGT12180.11144752770404368No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11980.10961751903895264No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11930.10916001687267989No Hit
GCATAGACGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC11860.10851951383989804No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11780.10778751037386163No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11750.10751300907409797No Hit
CCATTAATGCATTCGGGCCCTCTCGCACAGTAATAAACAGCCGTGTCCTC11710.10714700734107975No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCACCCTGGTCC11580.1059575017087706No Hit
GGGTTCCCTGGCCCCAAAAGTCGAACCAGCGTGGCGCAAAATGACAGATACCATTAATGCATTCGGGCCCTCTCG11540.10559149997575237No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11500.10522549824273418No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11470.10495099694297053No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11420.10449349477669777No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT11390.10421899347693411No Hit
GGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG11330.1036699908774068No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11290.10330398914438861No Hit
GCACTGAGCACAGAGGACTCACCATGGAGCTGGGACTGTGCTGGGTTTTC11130.10183998221231577No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11100.10156548091255213No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)