FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1092891
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCCTCGGCTGTCACGCTGCTCATCTGCAGATACACTGAGTCTTTGGTGTTGTCTCTGGA45220.4137649591770817No Hit
GTTTATTACTGTGCGAGAGGGCCCGAATGCATTAATGGTATCTGTCATTT41050.37560927850993375No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34830.31869600902560274No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26090.23872463036112476No Hit
GTATGATGACTGGCCTCGAGCGTTCGGCCAAGGGACCAGGGTGGAGGTCAAACGAACTGTGGCTGCACCAT24300.22234605280856004No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23650.21639852464701423No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC21670.198281438862613No Hit
GAGCTGGGACTGTGCTGGGTTTTCCTTGTCGCTATTTTAGAAGGTGTCCAATGTGAGATACAACTTGTGGAGT20280.18556287864023036No Hit
GTGCTGGGTTTTCCTTGTCGCTATTTTAGAAGGTGTCCAATGTGAGATAC20160.18446487344117574No Hit
GTCATCATACTGCTGACAGTAATAAACTGCAGAATCTTCAGGCTGCACGC19820.18135385871052098No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19600.17934084917892085No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19110.17485732794944783No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19030.17412532448341142No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18750.17156331235228398No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18670.17083130888624756No Hit
CTCCTGTGCAGCCTCTGGATTCACATTTAGGGATTTTGCGATGAACTGGG18510.16936730195417474No Hit
CGTCTATGCTGCATCCACCGGGACCACTGGTCTGGCATCAAGGTTCAGTGGCAGTGGGTCTGGAACAGAGTTCAC17580.16085776166150145No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17130.15674024216504664No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17020.1557337373992466No Hit
GAGTTCACCCTCACCATCACCAGCGTGCAGCCTGAAGATTCTGCAGTTTATTACTGTCAGCAGTATGATGACTGG16830.15399522916741012No Hit
GTTTATTACTGTCAGCAGTATGATGACTGGCCTCGAGCGTTCGGCCAAGGGACCAGGGTGGAGGTCAAACGAA16640.15225672093557363No Hit
CTCCTGCAGGGCCAGTGCGAGTATTAGGAGCAATTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT16580.15170771833604632No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16550.15143321703628268No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16320.14932870707142798No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGGGCCACCCTCTCCTGCAGGGCCAGTGCGAGTATTAGGAG16020.14658369407379143No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16020.14658369407379143No Hit
GTCTGGAACAGAGTTCACCCTCACCATCACCAGCGTGCAGCCTGAAGATT15700.14365568020964578No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15460.14145966981153657No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15350.1404531650457365No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15240.13944666027993644No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14720.13468863775069975No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14700.13450563688419065No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14640.13395663428466334No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14630.13386513385140877No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14600.13359063255164513No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14560.13322463081862693No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGCTGGGACTGTGCTGGG14470.13240112691933595No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14350.13130312172028136No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14070.1287411095891539No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13990.1280091061231175No Hit
GTCTGTGTCTCCAGGGGAAAGGGCCACCCTCTCCTGCAGGGCCAGTGCGAGTATTAGGAGCAATTTAGCCTGGTA13940.12755160395684476No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13920.12736860309033562No Hit
GGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG13850.12672810005755378No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13700.1253555935587355No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC13450.1230680827273717No Hit
GTAATAAACTGCAGAATCTTCAGGCTGCACGCTGGTGATGGTGAGGGTGAACTCTGTTCCAGACCCACTGCCA13430.12288508186086261No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13300.12169557622855344No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13030.11922506453068056No Hit
GTCCAGAGACAACACCAAAGACTCAGTGTATCTGCAGATGAGCAGCGTGACAGCCGAGGACACGGCTGTTTATTA12870.11776105759860774No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12630.11556504720049851No Hit
GGGTTCCCTGGCCCCAAAAGTCGAACCAGCGTGGCGCAAAATGACAGATACCATTAATGCATTCGGGCCCTCTCG12410.11355203766889838No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11880.10870251470640714No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC11870.10861101427315258No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11810.10806201167362528No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11740.1074215086408434No Hit
GTTCCAGACCCACTGCCACTGAACCTTGATGCCAGACCAGTGGTCCCGGT11730.10733000820758885No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11730.10733000820758885No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGCGACCAGGGTTCCCTGGCCCCAAAAGTCGAACCAGCGTGGCG11720.10723850777433432No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11660.106689505174807No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCACCCTGGTCC11510.10531699867598875No Hit
GTGCAGCCACAGTTCGTTTGACCTCCACCCTGGTCCCTTGGCCGAACGCT11460.10485949650971596No Hit
GCATAGACGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC11440.10467649564320687No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11430.1045849952099523No Hit
GGGTGACACAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGGGCC11380.10412749304367956No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11290.10330398914438861No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11190.10238898481184308No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG11000.1006504765800066No Hit
GGGCCACCCTCTCCTGCAGGGCCAGTGCGAGTATTAGGAGCAATTTAGCCTGGTACCAGCAGAAACCTGGCCAGG10970.10037597528024295No Hit
GCACTGAGCACAGAGGACTCACCATGGAGCTGGGACTGTGCTGGGTTTTC10940.10010147398047929No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGTG300.002798492366.0609170
GTGGTAT3050.044.7724381
GGTATCA10700.040.8391531
TACGTAT700.001668543637.74909270
GTATCAA26800.036.4318961
TATCAAC29500.033.202542
ATCAACG30300.032.3199843
TCAACGC30500.031.9961764
AACGCAG31200.031.2783156
CAACGCA31250.031.2282685
AGCCGTG11700.030.61932410
CAGCCGT11750.030.4890299
CCGTGTC11800.030.35844812
ACGTTTG450.004874289530.3360416
ACGCAGA32700.029.7391827
ATACGTA1151.9190338E-929.66705311
GATACAC12250.029.50061645
CGCAGAG33200.029.4955048
GCCGTGT12150.029.48392311
ACGCTGC11950.029.23189729
Produced by FastQC (version 0.11.5)