FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1835140
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC67920.3701080026591977No Hit
GCCTTGGGCTGACTGAGGACGGTCACCCTGGTCCCTCCGCCGAAAAGTCTCGAATTAGTGCCTGAAAATGAGCAG54980.29959567117495123No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT52480.2859727323255991No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45560.24826443759059252No Hit
GGACACAGGTTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCATGTCCGGGTCTCCTGGACAGTCGATCAC43530.23720261124491865No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAGGACGGTCACCCTGGTCCCTC41890.2282659633597437No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAGTCAGTAGTGACGTT41120.22407009819414322No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT40110.21856643089900496No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG36080.1966062534738494No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC35510.19350022341619713No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT33530.1827108558475103No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31850.17355624094074568No Hit
GTCCAAGGTGGGTCCTTCAGCGGCTACTACTGGACCTGGGTCCGCCAGTC31610.17224843881120785No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG31520.1717580130126312No Hit
GTCGATCACCATCTCCTGCACTGGAGTCAGTAGTGACGTTGGGAGTTATCGCCTTGTCTCCTGGTACCGGCAGTA30910.16843401593338928No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC30630.16690824678226185No Hit
GACTCAGCCTGCCTCCATGTCCGGGTCTCCTGGACAGTCGATCACCATCT30290.16505552709874996No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA29990.1634207744368277No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT29780.16227644757348214No Hit
CCCTGGTCCCTCCGCCGAAAAGTCTCGAATTAGTGCCTGAAAATGAGCAG29040.15824405767407393No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT28850.15720871432152317No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG28020.15268589862353826No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA27900.15203199755876937No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC27890.15197750580337194No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT27880.15192301404797454No Hit
GAAATAAAGAGCCGTGTCCGCGGCGGTCACAGAAGTCAAAGTCAGGGAGAAGTGGTTCTTGGACGTGTCTGTGGA27840.1517050470263849No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27520.14996131085366785No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA27410.14936190154429635No Hit
CTCCTGCACTGGAGTCAGTAGTGACGTTGGGAGTTATCGCCTTGTCTCCTGGTACCGGCAGTACCCAGGCAAAGC27340.14898045925651449No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26980.14701875606220777No Hit
GGCCTGGGCTCTGCTGCTCCTCATTCTCCTCACTCAGGACACAGGTTCCTGGGCCCAGTCTGCCCTGACTCAGC26360.14364026722756845No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG26320.14342230020597885No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG25850.14086118770230063No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT25020.13633837200431573No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC24830.135303028651765No Hit
GACTGAGGACGGTCACCCTGGTCCCTCCGCCGAAAAGTCTCGAATTAGTGCCTGAAAATGAGCAGCAAAAATAAT24810.13519404514097016No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG24690.13454014407620127No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24600.1340497182776246No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT23270.12680231480976928No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22800.12424120230609109No Hit
CCCCTGAGGGCCGTTGACTGGTCTCATAAATAATGAGTTTGGGGGCTTTGCCTGGGTACTGCCGGTACCAGGAGA22750.12396874352910403No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22730.12385976001830924No Hit
GCCCTGACTCAGCCTGCCTCCATGTCCGGGTCTCCTGGACAGTCGATCAC22290.12146212278082327No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22280.12140763102542586No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC21930.11950041958651655No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAGGACGGTCACC21810.11884651852174767No Hit
GGACCAGGGTGACCGTCCTCAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG21740.1184650762339658No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21720.11835609272317098No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT21410.11666684830585132No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCATGTCCGGGTCTCCTGGACAGTCG21240.11574048846409539No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA21230.11568599670869799No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21010.11448717808995498No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAGGACGGTCACCCTGGTC20950.11416022755757053No Hit
CCTCAGGGGTCTCTAATCGATTCTCTGGCTCCACGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGC20710.11285242542803274No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20620.11236199962945607No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20520.11181708207548197No Hit
CTACTGGACCTGGGTCCGCCAGTCCCCGGGTCAGGGGCTGGAGTGGGTTGCAGAAATCAATCATAATGGAGTCAC20360.11094521398912344No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG20360.11094521398912344No Hit
GTAGTGACGTTGGGAGTTATCGCCTTGTCTCCTGGTACCGGCAGTACCCA20310.1106727552121364No Hit
GGGTGACCGTCCTCAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAG20300.110618263456739No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT20200.11007334590276491No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20160.10985537888117529No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC20050.10925596957180378No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC20020.10909249430561156No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC20000.10898351079481675No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC20000.10898351079481675No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT19990.10892901903941934No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19910.10849308499624007No Hit
GTCAACGGCCCTCAGGGGTCTCTAATCGATTCTCTGGCTCCACGTCTGGC19730.10751223339908672No Hit
GGGCTCTGCTGCTCCTCATTCTCCTCACTCAGGACACAGGTTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT19700.1073487581328945No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC19610.10685833233431781No Hit
GGCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACGTGGAGCC19600.1068038405789204No Hit
GCACTGGAGTCAGTAGTGACGTTGGGAGTTATCGCCTTGTCTCCTGGTAC19510.10631341478034373No Hit
TCCTCAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19420.10582298898176706No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19140.10429721983063964No Hit
CGGTCACCCTGGTCCCTCCGCCGAAAAGTCTCGAATTAGTGCCTGAAAATGAGCAGCAAAAATAATCAGCCTCGT18970.10337085998888369No Hit
TCTCTGGGCTCCAGCCTGAGGACGAGGCTGATTATTTTTGCTGCTCATTT18920.10309840121189665No Hit
CTTTATTTCTGTGCGAGAGGACCGTCACTAAAATATTGTAGTAGTGCCAGCTGCCGATACTTCTATTTCTTCTA18470.10064627221901326No Hit
GCTCCAGCCTGAGGACGAGGCTGATTATTTTTGCTGCTCATTTTCAGGCA18410.1003193216866288No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA18390.10021033817583401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGTA850.052.56379738
AGGGGGG6800.051.7035970
CGTCGAT200.00772032151.1276429
GTCGTAT950.047.0943339
GCGTATG306.347963E-445.94761740
GGTATCA16850.045.20821
TCGTATG5150.044.83238240
CGTATGC5500.043.9148841
TCTCGTA4300.043.16061838
CCCACGA6150.043.1238519
ACGAGAC6150.043.1238522
ATGCCGT5800.042.46261244
CCGTCTT5850.042.30432547
CCCTAAG6050.042.2346428
ATCTCCG6200.042.2276710
ATGTCGT1150.041.78253637
CATGTCG1150.041.7503836
ATCTCGT4550.041.4873737
TAAGACA6550.040.64985331
TATACAC6500.040.2786983
Produced by FastQC (version 0.11.5)