FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1835140
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72560.39539217716359515No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC64100.3492921520973877No Hit
GCCTTGGGCTGACTGAGGACGGTCACCCTGGTCCCTCCGCCGAAAAGTCTCGAATTAGTGCCTGAAAATGAGCAG51330.27970618045489715No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50510.2752378565123097No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48760.2657017993177632No Hit
GGACACAGGTTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCATGTCCGGGTCTCCTGGACAGTCGATCAC42100.22941029022308926No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG41480.22603180138844994No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAGGACGGTCACCCTGGTCCCTC39210.21366217291323822No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAGTCAGTAGTGACGTT38000.2070686705101518No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT36100.19671523698464424No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC34690.18903189947360965No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33450.182274921804331No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT33430.18216593829353617No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG32450.17682574626459016No Hit
GTCCAAGGTGGGTCCTTCAGCGGCTACTACTGGACCTGGGTCCGCCAGTC31510.1717035212572338No Hit
GTCGATCACCATCTCCTGCACTGGAGTCAGTAGTGACGTTGGGAGTTATCGCCTTGTCTCCTGGTACCGGCAGTA29340.15987881033599619No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC28950.15775363187549724No Hit
CCCTGGTCCCTCCGCCGAAAAGTCTCGAATTAGTGCCTGAAAATGAGCAG28650.15611887921357498No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27990.15252242335734603No Hit
CTCCTGCACTGGAGTCAGTAGTGACGTTGGGAGTTATCGCCTTGTCTCCTGGTACCGGCAGTACCCAGGCAAAGC27790.15143258824939787No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27740.1511601294724108No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA27550.15012478611986008No Hit
GACTCAGCCTGCCTCCATGTCCGGGTCTCCTGGACAGTCGATCACCATCT27280.14865350872413002No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT26900.14658282201902853No Hit
GGCCTGGGCTCTGCTGCTCCTCATTCTCCTCACTCAGGACACAGGTTCCTGGGCCCAGTCTGCCCTGACTCAGC26710.14554747866647777No Hit
GAAATAAAGAGCCGTGTCCGCGGCGGTCACAGAAGTCAAAGTCAGGGAGAAGTGGTTCTTGGACGTGTCTGTGGA26110.14227797334263326No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG25250.13759168237845615No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25220.13742820711226392No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT25080.1366653225367002No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC24600.1340497182776246No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC24200.13187004806172825No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24180.13176106455093345No Hit
GGGTGACCGTCCTCAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAG23830.12985385311202413No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT23330.12712926534215374No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23220.12652985603278225No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC23170.1262573972557952No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22970.12516756214784702No Hit
GGGCTCTGCTGCTCCTCATTCTCCTCACTCAGGACACAGGTTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT22830.12440467757228331No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22280.12140763102542586No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22070.12026330416208028No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21980.11977287836350362No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21920.11944592783111915No Hit
CCCCTGAGGGCCGTTGACTGGTCTCATAAATAATGAGTTTGGGGGCTTTGCCTGGGTACTGCCGGTACCAGGAGA21730.11841058447856839No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21600.11770219165840208No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21600.11770219165840208No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21550.11742973288141503No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAGGACGGTCACCCTGGTC21300.11606743899647982No Hit
GCCCTGACTCAGCCTGCCTCCATGTCCGGGTCTCCTGGACAGTCGATCAC21170.11535904617631353No Hit
GACTGAGGACGGTCACCCTGGTCCCTCCGCCGAAAAGTCTCGAATTAGTGCCTGAAAATGAGCAGCAAAAATAAT21150.11525006266551871No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA20850.11361531000359645No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAGGACGGTCACC20810.11339734298200681No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC20790.11328835947121202No Hit
CTTTATTTCTGTGCGAGAGGACCGTCACTAAAATATTGTAGTAGTGCCAGCTGCCGATACTTCTATTTCTTCTA20670.1126344584064431No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCATGTCCGGGTCTCCTGGACAGTCG20530.11187157383087938No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT19990.10892901903941934No Hit
GGACCAGGGTGACCGTCCTCAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG19970.10882003552862451No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19940.10865656026243228No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC19930.10860206850703488No Hit
GGCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACGTGGAGCC19680.10723977462209967No Hit
CCTCAGGGGTCTCTAATCGATTCTCTGGCTCCACGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGC19670.10718528286670227No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19380.10560502196017743No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19190.10456967860762666No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18950.10326187647808888No Hit
CTACTGGACCTGGGTCCGCCAGTCCCCGGGTCAGGGGCTGGAGTGGGTTGCAGAAATCAATCATAATGGAGTCAC18930.10315289296729405No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC18790.10239000839173033No Hit
GCACTGGAGTCAGTAGTGACGTTGGGAGTTATCGCCTTGTCTCCTGGTAC18770.10228102488093552No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC18760.1022265331255381No Hit
TCTCTGGGCTCCAGCCTGAGGACGAGGCTGATTATTTTTGCTGCTCATTT18550.10108220626219253No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT18530.10097322275139772No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18500.10080974748520549No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC200.00782649550.9512943
TATCGTC200.00782986850.945745
GGTATCA20150.041.834691
GTGGTAT6050.038.7662351
GTATCAA47250.035.24961
TGGTATC6700.034.9872062
CGCTTAA400.002588383934.52264441
TACGTAT800.002697053134.2324970
TATCAAC52850.031.0482462
ATCAACG52750.031.0376263
ACGCTTA450.004644899630.63757340
TCAACGC53450.030.6269744
ACGAACC2450.028.74466752
CGCAGAG57850.028.3570048
GCTTAGA605.916477E-428.3255711
ACGCAGA58350.028.1140147
CGGCAAA2005.368838E-727.38599470
CAGAGTA59800.027.37552610
AGAGTAC59700.027.36374311
ACAGGGG3900.027.0182361
Produced by FastQC (version 0.11.5)