FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505650
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT108910.723342078172218No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG46780.3106963769800419No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA34090.22641384119815364No Hit
CTGTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGATGAGAGTGAAGTCTGTC32200.21386112310297878No Hit
GTATAACAGTGACCCGCTCACTTTCGGCGGCGGGACCAAGGTGGAGATCAAGGGAACTGTGGCTGCACCAT30880.20509414538571383No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27970.18576694450901604No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT27180.18052004117822867No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC26590.17660146780460267No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26080.1732142264138412No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG25840.17162023046524758No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG25290.16796732308305384No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC25240.1676352405937635No Hit
GTCCTTTTACTCCCCGAATTAGAATTAAGGCGCATCTCACACAGAAATAAACAGCCGTGTCCTCGACTCTCAGG25080.1665725766280344No Hit
GTATATGTGATTACTAGCAGCAGAAATGGATGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT23560.15647726895360806No Hit
GAAATAAACAGCCGTGTCCTCGACTCTCAGGCTGTTCATCTGCAGATACA23530.1562780194600339No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC23430.15561385448145318No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC22990.1526915285756982No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT22420.14890578819778832No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAATAAGTTTAGCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG22260.14784312423205925No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21330.14166638993125893No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC20770.13794706605120713No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT20670.13728290107262645No Hit
CACATATACTACGCAGACTCAGTGAAGGGCCGATTCACCGTCTCCAGAGACAACGCCAAGGCCTCACTGTATCTG20470.13595457111546508No Hit
GATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGCTTCAGCGGCGGTGGATCTGGGACAGACTTCA20390.13542323913260054No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT19880.13203599774183908No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT19870.131969581243981No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGT19740.13110616677182613No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC19700.13084050078039386No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC19440.12911367183608408No Hit
GCCTTAGCCCTGGATTCCAGGGCCTATCCACTTGGTGATCAGCACTGAGC19400.1288480058446518No Hit
GTAAAAGGACCGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGGAGTGCATCCGCCCCAACC19240.12778534187892274No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT18760.12459734998173547No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG18640.12380035200743865No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT18580.12340185302029023No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC18550.12320260352671603No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC18390.12213993956098695No Hit
CCTGTGAGGTGGCTGCGTACTTGCCCCCTCTCAGGACTGATGGGAAGCCC18170.12067877660810945No Hit
GTTTATTTCTGTGTGAGATGCGCCTTAATTCTAATTCGGGGAGTAAAAGG17980.11941686314880617No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT17760.11795570019592866No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC17540.11649453724305117No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16710.11098196792083154No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16320.10839172450436689No Hit
GGGTTCCCTGGCCCCAGGGGTCGAACGGTCCTTTTACTCCCCGAATTAGA16270.10805964201507653No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACGGTC16020.10639922956862484No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15890.10553581509646996No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT15820.10507089961146349No Hit
GACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTG15810.10500448311360543No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15690.10420748513930861No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATGCTAAACTTAT15600.10360973665858599No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA15530.10314482117357952No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15360.10201574070999236No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA15360.10201574070999236No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTT15320.1017500747185601No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGACATTAGCAATTCTTTAGCCTGGTATCAGCAGACA15280.10148440872712781No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)