FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505650
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG198871.3208248929033972No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG46610.3095672965164547No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA35870.2382359778168897No Hit
CTGTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGATGAGAGTGAAGTCTGTC31540.2094776342443463No Hit
GTATAACAGTGACCCGCTCACTTTCGGCGGCGGGACCAAGGTGGAGATCAAGGGAACTGTGGCTGCACCAT28690.19054893235479692No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28130.1868296084747451No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC27430.18218045362468038No Hit
GTCCTTTTACTCCCCGAATTAGAATTAAGGCGCATCTCACACAGAAATAAACAGCCGTGTCCTCGACTCTCAGG25920.1721515624481121No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC24000.15939959485936306No Hit
GTATATGTGATTACTAGCAGCAGAAATGGATGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT23640.15700860093647262No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAATAAGTTTAGCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG23470.15587952047288547No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22890.15202736359711752No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG22690.15069903363995615No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT22340.1483744562149238No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22340.1483744562149238No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21640.14372530136485903No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT21360.14186563942483313No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC21020.13960747849765884No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC20950.13914256301265235No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC20710.1375485670640587No Hit
GAAATAAACAGCCGTGTCCTCGACTCTCAGGCTGTTCATCTGCAGATACA20710.1375485670640587No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT19990.1327665792182778No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT19290.12811742436821305No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT19200.12751967588749044No Hit
GTAAAAGGACCGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGGAGTGCATCCGCCCCAACC18950.12585926344103876No Hit
GATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGCTTCAGCGGCGGTGGATCTGGGACAGACTTCA18430.12240560555241922No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT18190.12081160960382559No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC18160.12061236011025138No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC18020.11968252914023843No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC17960.11928403015309003No Hit
CACATATACTACGCAGACTCAGTGAAGGGCCGATTCACCGTCTCCAGAGACAACGCCAAGGCCTCACTGTATCTG17890.11881911466808355No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT17280.1147677082987414No Hit
GCCTTAGCCCTGGATTCCAGGGCCTATCCACTTGGTGATCAGCACTGAGC17210.11430279281373493No Hit
GGGTTCCCTGGCCCCAGGGGTCGAACGGTCCTTTTACTCCCCGAATTAGA16930.11244313087370904No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA16750.1112476339122638No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC16560.10998572045296051No Hit
GTTTATTTCTGTGTGAGATGCGCCTTAATTCTAATTCGGGGAGTAAAAGG16260.10799322551721849No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16090.10686414505363133No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGT16020.10639922956862484No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15880.10546939859861189No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15880.10546939859861189No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTT15870.10540298210075384No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATGCTAAACTTAT15560.10334407066715373No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG15420.10241423969714075No Hit
CCTGTGAGGTGGCTGCGTACTTGCCCCCTCTCAGGACTGATGGGAAGCCC15250.10128515923355362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3000.044.7093661
CCTAACG307.277578E-444.6900522
GTATCAA22700.034.7137681
CTAACGC400.00299555633.5086333
TATCAAC26200.030.0634442
ATCAACG27150.029.127213
TCAACGC27050.028.9861764
ATACGTA1402.1827873E-1128.71882611
CAACGCA27400.028.6140165
AACGCAG27600.028.285276
GACAGGT2750.028.0449641
AATACGT1453.274181E-1127.72852110
TTCGATC500.00783831827.5254939
CGCAGAG28600.027.1791218
AGAGTAC29100.026.94240411
CAGAGTA29050.026.8734410
CTAAACC757.51995E-526.8060174
GAATACG1505.0931703E-1126.8042379
TAGGGTG500.00892225426.8042375
GCAGAGT29550.026.4187289
Produced by FastQC (version 0.11.5)