FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737896
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32110.4351561737697453No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCAC29370.39802356971714176No Hit
CTCCTGCACTGGGGGCAGCTCCAATATCGGGGCAGGTTATGATGTTTATTGGTACCAGCACCTTCCAGGAACAGC28560.3870464130446567No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT26040.3528952589524811No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24740.3352776000954064No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23850.3232162798009476No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20980.28432190986263645No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC20510.27795244858354023No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACAAAACCACTC20310.27524203952860565No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19930.27009226232423No Hit
GTCATAGGACTGGCAGTAATAATCACCCTCATCCTCAGCCTGGAGCCCCG19000.2574888602187842No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18720.25369428754187584No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17590.2383804763814955No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16460.22306666522111518No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16330.2213048993354077No Hit
GTCCTATGACAGCAGCCTGAGTGGTTTTGTGATATTCGGCGGAGGGACCA16010.21696824484751237No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15990.21669720394201894No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15920.2157485607727918No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14930.2023320359508657No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14580.1975888201047302No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14400.1951494519552891No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14010.18986415429816667No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13860.18783134750696576No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13820.18728926569597884No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT13780.18674718388499192No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13760.18647614297949847No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13700.1856630202630181No Hit
TCCAGGAACAGCCCCCAAACTCCTCAATTATGTTAACAACAATCGGCCCTCAGGGGTCCCTGAGCGCTTCTCTGC13470.18254604984984335No Hit
GGTTATGATGTTTATTGGTACCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCAATTATGTTAACAACAATCGG13350.18091980441688257No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13050.17685419083448073No Hit
GGATGAGGGTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTT12700.17211097498834524No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA12700.17211097498834524No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12100.16397974782354155No Hit
GCTCCAGGCTGAGGATGAGGGTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTTTGTGATATTCGG12030.16303110465431442No Hit
GGGCAGAAGGTCACCATCTCCTGCACTGGGGGCAGCTCCAATATCGGGGCAGGTTATGATGTTTATTGGTACCAG11970.16221798193783407No Hit
GTGTCTGGGGCCCCAGGGCAGAAGGTCACCATCTCCTGCACTGGGGGCAG11830.16032069559937984No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11760.15937205243015276No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACAAAACCACTCA11670.1581523683554322No Hit
CCCTCATCCTCAGCCTGGAGCCCCGTGATGGCCAGGGAGGCTGAGATGCC11540.15639060246972475No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11480.15557747975324435No Hit
GCCCCCAAACTCCTCAATTATGTTAACAACAATCGGCCCTCAGGGGTCCCTGAGCGCTTCTCTGCCTCCAAGT11170.15137634571809577No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11120.15069874345436213No Hit
GTGCAGGAGATGGTGACCTTCTGCCCTGGGGCCCCAGACACTGAGGGCGG10910.14785281394668084No Hit
GCCTGGAGCCCCGTGATGGCCAGGGAGGCTGAGATGCCAGACTTGGAGGC10760.1458200071554799No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10650.1443292821752659No Hit
GTACCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCAATTATGTTAACA10480.14202543447857152No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10300.1395860663291304No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC10270.1391795049708902No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10010.13565597319947525No Hit
GTGTATTACTGTGCGAGGGTGCCAGTTGCAAGCCACGCCGTTGACTCCTGGGGCCAGGGAATCCTGGTCACCGT9920.13443628912475472No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9740.1319969209753136No Hit
CTCCAATATCGGGGCAGGTTATGATGTTTATTGGTACCAGCACCTTCCAG9580.12982859373136593No Hit
GTTATGATGTTTATTGGTACCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCAATTATGTTAACAACAATCGGC9490.1286089096566454No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9470.1283378687511519No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9390.1272537051291781No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC9380.12711818467643138No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9170.12427225516875007No Hit
GCCCTCAGGGGTCCCTGAGCGCTTCTCTGCCTCCAAGTCTGGCATCTCAG9120.12359465290501642No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTTTGTGATATTCGGCGGAGGGACCAAGCTGACCGTC9030.12237496883029587No Hit
GTTCCTGGAAGGTGCTGGTACCAATAAACATCATAACCTGCCCCGATATT8920.12088424385008184No Hit
GCTTGGTCCCTCCGCCGAATATCACAAAACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCACCCTCAT8880.12034216203909494No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8850.11993560068085476No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC8840.11980008022810802No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8740.11844487570064074No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC8660.11736071207866693No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACAAAACCA8630.11695415072042674No Hit
GTTTGGGGGCTGTTCCTGGAAGGTGCTGGTACCAATAAACATCATAACCT8290.11234645532703796No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT8270.1120754144215445No Hit
TCACAAAACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCACCC8090.10963604627210338No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8030.10882292355562301No Hit
GGCCATCACGGGGCTCCAGGCTGAGGATGAGGGTGATTATTACTGCCAGT8020.10868740310287629No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7980.10814532129188936No Hit
GTTTTGTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC7980.10814532129188936No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA7900.10706115766991554No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7890.10692563721716881No Hit
GTAATAATCACCCTCATCCTCAGCCTGGAGCCCCGTGATGGCCAGGGAGG7890.10692563721716881No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG7790.10557043268970152No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC7750.10502835087871462No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC7730.10475730997322116No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7720.10462178952047442No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7570.10258898272927351No Hit
CCCTCAGTGTCTGGGGCCCCAGGGCAGAAGGTCACCATCTCCTGCACTGG7430.10069169639081932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4200.060.1986570
AGACCGG200.00651242153.3721752
GTCTATT200.008096150.513591
GTCGTAT750.050.46188739
ATGTCGT800.047.1122237
GGTATCA8300.046.659141
CGTTTGC306.239786E-446.10437441
TGTCGTA851.8189894E-1244.42365338
GTGGTAT1950.043.1740071
CTTGAAA5100.042.3888757
CGTATGC4900.042.3407541
ATGCCGT4800.042.0838444
TATACAC5700.041.938433
TCGTATG4700.041.8421540
TTATACA5500.041.626982
CCCACGA5500.041.62133819
CTTATAC5600.041.493311
GCCGTCT4750.041.2646
CCGTCTT4950.041.08222247
TAAGACA5400.040.792831
Produced by FastQC (version 0.11.5)