FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737896
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59380.8047204484100741No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31220.4230948534752865No Hit
CTCCTGCACTGGGGGCAGCTCCAATATCGGGGCAGGTTATGATGTTTATTGGTACCAGCACCTTCCAGGAACAGC28580.38731745395015016No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCAC25590.34679683857887833No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAAGGTCACCATCTCCTG25230.3419181022799961No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23960.32470700478116155No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22040.2986870778537897No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20690.28039181673298136No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19780.26805945553302907No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17880.24231056951115063No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC17850.24190400815291047No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17310.23458590370458707No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16920.2293006060474647No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16650.225641553823303No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15870.21507095850905816No Hit
GTCCTATGACAGCAGCCTGAGTGGTTTTGTGATATTCGGCGGAGGGACCA15420.2089725381354554No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACAAAACCACTC15270.20693973134425447No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAAGGTCAC15170.20558452681678718No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT14540.1970467382937433No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14430.19555601331352926No Hit
GTCATAGGACTGGCAGTAATAATCACCCTCATCCTCAGCCTGGAGCCCCG14110.19121935882563396No Hit
GGTTATGATGTTTATTGGTACCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCAATTATGTTAACAACAATCGG13920.18864447022344613No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13570.18390125437731064No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13510.18308813166083024No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13480.18268157030259005No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13430.1820039680388564No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13190.17875147717293494No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAAGGTCAC13090.17739627264546767No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12870.1744148226850396No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA12720.1723820158938387No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12650.1714333727246116No Hit
TCCAGGAACAGCCCCCAAACTCCTCAATTATGTTAACAACAATCGGCCCTCAGGGGTCCCTGAGCGCTTCTCTGC12620.1710268113663714No Hit
GGATGAGGGTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTT12350.16736775914220975No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12230.165741513709249No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT11970.16221798193783407No Hit
GGGCAGAAGGTCACCATCTCCTGCACTGGGGGCAGCTCCAATATCGGGGCAGGTTATGATGTTTATTGGTACCAG11880.16099829786311348No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11540.15639060246972475No Hit
GCTCCAGGCTGAGGATGAGGGTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTTTGTGATATTCGG11110.1505632230016154No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10920.14798833439942755No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10550.14297407764779863No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10430.14134783221483788No Hit
GCCCCCAAACTCCTCAATTATGTTAACAACAATCGGCCCTCAGGGGTCCCTGAGCGCTTCTCTGCCTCCAAGT10400.1409412708565977No Hit
GTGTCTGGGGCCCCAGGGCAGAAGGTCACCATCTCCTGCACTGGGGGCAG10320.13985710723462386No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10160.1376887799906762No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10130.137282218632436No Hit
GTGCAGGAGATGGTGACCTTCTGCCCTGGGGCCCCAGACACTGAGGGCGG10010.13565597319947525No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC9960.1349783709357416No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9910.134300768672008No Hit
GTACCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCAATTATGTTAACA9730.13186140052256687No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGC9700.13145483916432668No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9660.13091275735333976No Hit
GTTATGATGTTTATTGGTACCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCAATTATGTTAACAACAATCGGC9530.1291509914676323No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9500.1287444301093921No Hit
GTGTATTACTGTGCGAGGGTGCCAGTTGCAAGCCACGCCGTTGACTCCTGGGGCCAGGGAATCCTGGTCACCGT9410.12752474603467157No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9310.12616954150720427No Hit
CTCCAATATCGGGGCAGGTTATGATGTTTATTGGTACCAGCACCTTCCAG9200.12467881652699025No Hit
GCCTGGAGCCCCGTGATGGCCAGGGAGGCTGAGATGCCAGACTTGGAGGC9130.12373017335776315No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTTTGTGATATTCGGCGGAGGGACCAAGCTGACCGTC9100.12332361199952296No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT9070.12291705064128278No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC9050.12264600973578932No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9050.12264600973578932No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8770.11885143705888093No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8730.118309355247894No Hit
GTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAAGGTCACCATCTCCTGCACTGGGGGCAG8500.11519238483471926No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACAAAACCACTCA8480.1149213439292258No Hit
GTTTTGTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC8160.11058468944133049No Hit
GTCTGGGGCCCCAGGGCAGAAGGTCACCATCTCCTGCACTGGGGGCAGCT8080.10950052581935665No Hit
GGCCATCACGGGGCTCCAGGCTGAGGATGAGGGTGATTATTACTGCCAGT8060.1092294849138632No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7970.10800980083914265No Hit
CCCTCATCCTCAGCCTGGAGCCCCGTGATGGCCAGGGAGGCTGAGATGCC7950.10773875993364919No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA7920.107332198575409No Hit
GGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAAGGTCACCATCTC7860.10651907585892864No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7790.10557043268970152No Hit
GCCCTCAGGGGTCCCTGAGCGCTTCTCTGCCTCCAAGTCTGGCATCTCAG7720.10462178952047442No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC7710.1044862690677277No Hit
GCTTGGTCCCTCCGCCGAATATCACAAAACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCACCCTCAT7620.10326658499300716No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC7620.10326658499300716No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7580.10272450318202023No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACAAAACCA7560.10245346227652677No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT7550.10231794182378004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)