FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299154
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATATTGGTCATCTATGAAGATGCCAGGCGGCCCTCAGGGATCCCT39480.3038900699993996No Hit
GTCTATGGATGAGGCTGATTATTACTGTCAGGCGTGGGACAGTGACACTGTGGTGTTCGGCGGAGGGACCAAG36320.2795665486924568No Hit
CAATAATACATGGCCGTGTCCTCGGCTCTCAGGCTGCTCATGTGTAGAAT35210.27102252696754964No Hit
GTATTATTGTGTGAAAGGACACAGCGCTGGCTGGACTTACCTTACGTTCTGGGGCCAGGGAACCCTGGTCACCGT34390.26471072713473537No Hit
TCCATAGACTGGGTGTCGCTGACGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGGCCAGGGATC29780.22922609636732827No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTCTGGGCTGAGGTGGCTTTTT28850.22206759167889256No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26690.20544138724123542No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24990.19235594856344976No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24950.19204805588867832No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC24620.18950794132181406No Hit
GTGTGAGGTGCAGTTGTTGGACTCTGGGGGAGGCTTGGTGCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGATGC23910.18404284634462117No Hit
GGCCAGGCCAGTCCCCTATATTGGTCATCTATGAAGATGCCAGGCGGCCC23810.1832731146576926No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23520.18104089276559976No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22580.1738054149084712No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTTTCCCCAGGACAGACA22310.17172713935376407No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22130.17034162231729263No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21980.1691870247868998No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21860.1682633467625855No Hit
ATATAGGGGACTGGCCTGGCCTCTGCTGATACCACGAAGCAAACTTATCC21740.16733966873827122No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21670.16680085655742122No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20930.1611048420741498No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGGAGGACGGTCAGCTTGGTCCCTC20890.16079694939937836No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20500.15779499582035694No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20390.1569482909647355No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19880.1530226593613998No Hit
CTCCTGTGATGCCTCTGGATTCACCTTTGATAAATATGCCATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG19660.15132924965015696No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19610.15094438380669267No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19430.14955886677022123No Hit
GCCTTGGGCTGACGGAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAC18890.14540231566080697No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18840.1450174498173427No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18820.14486350347995694No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18590.14309312060002125No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18260.14055300603315696No Hit
GATTATTACTGTCAGGCGTGGGACAGTGACACTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTCCGTCAG17960.13824381097237126No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16890.13000768192223552No Hit
TCTATGAAGATGCCAGGCGGCCCTCAGGGATCCCTGGCCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACT16830.1295458429100784No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC16740.12885308439184268No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT16300.12546626496935698No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15940.12269523089641413No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG15930.12261825772772127No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15720.12100182118517128No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15630.12030906266693554No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15630.12030906266693554No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15290.11769197493137842No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT15130.11646040423229272No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15020.11561369937667129No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14960.11515186036451412No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14920.1148439676897427No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14850.1143051555088927No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14610.11245779946026414No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14560.11207293361679986No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG14550.111995960448107No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14440.11114925559248558No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14390.1107643897490213No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAACGTAAGGTAAGTCCAGC14350.11045649707424984No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14270.109840711724707No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC14020.10791638250738558No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13660.10514534843444272No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13600.10468350942228558No Hit
GGGTTCCCTGGCCCCAGAACGTAAGGTAAGTCCAGCCAGCGCTGTGTCCTTTCACACAATAATACATGGCCGTGT13540.10422167041012843No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC13470.10368285822927843No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13470.10368285822927843No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13470.10368285822927843No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG13450.10352891189189273No Hit
CCCTGAGACTCTCCTGTGATGCCTCTGGATTCACCTTTGATAAATATGCC13370.10291312654234987No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT13320.10252826069888558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30350.038.814671
TGGCGAA400.00273024134.14829324
TATCAAC34700.033.9331742
ATCAACG36500.032.536523
TCAACGC36800.032.0845764
CAACGCA37350.031.9763375
AACGCAG37850.031.4637766
TTATACT553.4730273E-431.0223944
TAACGGC902.0469088E-730.5778236
CGCAGAG39850.029.7145528
AGAGTAC40450.029.10394511
CAGAGTA41300.028.67019810
GCAGAGT41450.028.4841219
AGGTGAT11800.028.44186670
GCTTGGG2200.027.9373591
ATAGCGG1657.2759576E-1126.0050663
GTGCCCA16850.025.8930770
TAACCGT1109.913456E-725.52935853
GGTACCC1251.1712109E-725.03811646
TTAACGG1101.1929187E-624.99300235
Produced by FastQC (version 0.11.5)