FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186355
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT32841.7622280056880686No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC7750.41587293069678843No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC3750.20122883743392989No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3560.1910332430039441No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT3550.19049663277078693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.176008156475544No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.1518606959834724No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA2730.14649459365190093No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2710.14542137318558665No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG2530.135762388988758No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG2500.13415255828928657No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC2480.13307933782297227No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2470.13254272758981514No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG2390.12824984572455797No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC2360.12664001502508654No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG2330.1250301843256151No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2260.12127391269351505No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2230.11966408199404362No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC2210.11859086152772934No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.11429797966247215No Hit
GTAGGAGACAGAGTCATCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTATTTAGGCTGGTATCAGCAAAAA2110.11322475919615788No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT2090.11215153872984357No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT2030.1089318773309007RNA PCR Primer, Index 27 (95% over 23bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.10571221593195783No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT1950.10463899546564352No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT1930.10356577499932924No Hit
GAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGT1930.10356577499932924No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1930.10356577499932924No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.10195594429985778No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.10195594429985778No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTCTGCGTTGATAC1890.10141933406670066No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT1890.10141933406670066No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAA100.0061704833151.8085570
CGATCCG100.0061704833151.8085570
AGGTTCG150.001254360980.8387254
CTGGATA350.002962857565.060870
TCCCGAT150.003329127363.21138830
CAGATCG150.003577679962.0733827
TTATGTT200.003805298861.10660655
AATGAGA250.00459021958.2742668
TTGGTCG200.00524044656.36418546
ATGACGA250.00790633750.79486559
CTACCTT300.00944047548.56188668
AGGGGGG5500.048.30271570
AGTCCAC200.00998522747.88741331
ACCTAAC1350.047.5018461
ATACGGG457.870767E-847.5018461
CTTGAAA5550.047.20622657
AAGGGGG4000.046.67507669
CCGTCTT5600.043.96359647
CGTCTTC5600.043.7827848
TGCTTGA6050.043.09446355
Produced by FastQC (version 0.11.5)