FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307104
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40600.31061032633975566No Hit
GTATAACAGTGCCCCGCTCACTTTCGGCGGCGGGACCAAGGTGGAGATGAAACGAACTGTGGCTGCACCAT39230.3001291404509511No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37170.2843691091145005No Hit
CTGTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC34440.26348324234337894No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29590.22637831419688106No Hit
GATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCGGTGGCAGTGGATCTGGGACAGATTTCA27400.20962371777609126No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24210.18521862070653905No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAATAACGCCTGG23690.18124036036918256No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23610.18062832031728157No Hit
GTAATAGACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAAATACA22930.1754259798761231No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22920.17534947486963548No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22350.17098868949984086No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21730.16624537909760814No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAATCACCATCACTT21320.16310867383161556No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20540.15714128332558083No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG19810.15155641785198423No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19570.14972029769628126No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19260.14734864249516488No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19200.14688961245623913No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC18480.14138125198913015No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAATAACGCCTGGATAAGCTGGGTCCGCCAGGCTTCAGGGAAGGGG18400.14076921193722916No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18320.14015717188532817No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17920.13709697162582318No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17710.1354903664895831No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17650.13503133645065732No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC17560.1343427913922687No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT17410.13319521629495434No Hit
ACTTATTACTGTCAAAAGTATAACAGTGCCCCGCTCACTTTCGGCGGCGGGACCAAGGTGGAGATGAAACGAA17120.13097657110681324No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT16530.12646277572404338No Hit
GCATAGATCAGGAGCTCAGGAACTTTCCCTGGTTTCTGCTGATACCAGGC16320.12485617058780327No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATAGCAGATGTGACAT16270.12447364555536514No Hit
ACAGTGCCCCGCTCACTTTCGGCGGCGGGACCAAGGTGGAGATGAAACGA16080.12302005043210028No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15840.12118393027639729No Hit
GTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGC15760.1205718902244963No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG15420.11797072000391706No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG15180.11613459984821407No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15100.11552255979631308No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAAAGTATAACAGTGCC15040.11506352975738732No Hit
AGTCAGGGCATTAGCAATTATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTGAGCTCCTGATCTATG15030.1149870247508997No Hit
GTCTATTACTGTACCACAGGGCAGCGGAGTGGTTATTATACCTACTGGGGCCAGGGAACCCTGGTCACCGTCTC14940.11429847969251108No Hit
AAGTATAACAGTGCCCCGCTCACTTTCGGCGGCGGGACCAAGGTGGAGATGAAACGAACTGTGGCTGCACCAT14860.11368643964061009No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14760.11292138957573383No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14760.11292138957573383No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA14720.11261536954978334No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14620.1118503194849071No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14580.1115442994589566No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14530.11116177442651848No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14370.10993769432271648No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCCCGCCGCCGAAAGTGAGCGGGGCA14360.10986118931622885No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13930.106571474037261No Hit
GTGGTACAGTAATAGACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTG13890.10626545401131049No Hit
GTAGGAGACAGAATCACCATCACTTGCCGGGCGAGTCAGGGCATTAGCAATTATTTAGCCTGGTATCAGCAGAAA13320.10190466864151591No Hit
GCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATAGCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC13260.10144563860259015No Hit
GTCTGCATCTGTAGGAGACAGAATCACCATCACTTGCCGGGCGAGTCAGG13110.10029806350527579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)