FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307104
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42020.3214740372609983No Hit
GTATAACAGTGCCCCGCTCACTTTCGGCGGCGGGACCAAGGTGGAGATGAAACGAACTGTGGCTGCACCAT38800.29683942517198325No Hit
CTGTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC35150.2689150978040003No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30680.23471735990403214No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27700.21191886797072No Hit
GATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCGGTGGCAGTGGATCTGGGACAGATTTCA25010.191339021225549No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24000.18361201557029894No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23890.18277046049893506No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22800.17443141479178398No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21300.1629556638186403No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19880.1520919528973976No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAATAACGCCTGG19540.14949078267681837No Hit
GTAATAGACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAAATACA19510.14926126765735548No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAATCACCATCACTT19410.14849621759247925No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC18960.14505349230053616No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18850.14421193722917228No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18620.14245232207995692No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17890.13686745660636032No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17830.13640842656743457No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17660.13510784145714497No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG16970.12982899600949885No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16850.12891093593164735No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16810.12860491590569686No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16790.12845190589272162No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAATAACGCCTGGATAAGCTGGGTCCGCCAGGCTTCAGGGAAGGGG16490.1261567556980929No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16360.12516219061375378No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16160.12363209048400128No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16090.12309655543858791No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC16000.12240801038019929No Hit
GTGGTACAGTAATAGACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTG15920.12179597032829828No Hit
GTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCCCGCCGCCGAAAGTG15700.12011286018557053No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT15690.12003635517908293No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15620.11950082013366954No Hit
ACTTATTACTGTCAAAAGTATAACAGTGCCCCGCTCACTTTCGGCGGCGGGACCAAGGTGGAGATGAAACGAA15610.11942431512718193No Hit
GTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGC15450.11820023502337994No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15070.11529304477685019No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG14810.11330391460817196No Hit
GCATAGATCAGGAGCTCAGGAACTTTCCCTGGTTTCTGCTGATACCAGGC14540.1112382794330061No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14510.11100876441354324No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14400.11016720934217934No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAAAGTATAACAGTGCC14380.1100141993292041No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCCCGCCGCCGAAAGTGAGCGGGGCA14350.10978468430974124No Hit
GTCTATTACTGTACCACAGGGCAGCGGAGTGGTTATTATACCTACTGGGGCCAGGGAACCCTGGTCACCGTCTC14080.10771904913457536No Hit
ACAGTGCCCCGCTCACTTTCGGCGGCGGGACCAAGGTGGAGATGAAACGA13910.10641846402428576No Hit
AAGTATAACAGTGCCCCGCTCACTTTCGGCGGCGGGACCAAGGTGGAGATGAAACGAACTGTGGCTGCACCAT13730.1050413739075085No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13710.10488836389453327No Hit
GTATAATAACCACTCCGCTGCCCTGTGGTACAGTAATAGACGGCTGTGTC13640.10435282884911988No Hit
GTCTGCATCTGTAGGAGACAGAATCACCATCACTTGCCGGGCGAGTCAGG13570.10381729380370651No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13480.10312874874531791No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13450.10289923372585501No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACTGGC13440.1028227287193674No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATAGCAGATGTGACAT13340.10205767865449115No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG13170.10075709354420152No Hit
AGTCAGGGCATTAGCAATTATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTGAGCTCCTGATCTATG13110.10029806350527579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)