FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893362
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTTCGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCAT84360.9442980561071547No Hit
GTATAATATTGTTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTAATGGTGAGAGAGAAATCTGTC62050.6945672638863082No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42790.47897716715060634No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41550.46509701554353106No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29170.32651937288579547No Hit
CCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGGTGGAGCTGTAGAAAATACT28980.3243925754621307No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCA27640.3093930567899687No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26040.29148318374858123No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGACTGCTCATTTGCAGATACA25780.28857282937935574No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25620.286781842075217No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24550.2748046144787891No Hit
CTCCTAAGGTTCTCCTTTTCTGGGCATCCACCCGGGAATCCGGGGTCCCT23810.26652129819714737No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23490.2629393235888699No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22870.25599924778533223No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22850.25577537437231496No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22530.25219339976403743No Hit
CCATTAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAACAA22510.2519695263510201No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22120.2476039947971819No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20770.2324925394185112No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20560.2301418685818291No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20080.22476890666941282No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC17880.20014283123750506No Hit
AACCAGGACAGCCTCCTAAGGTTCTCCTTTTCTGGGCATCCACCCGGGAA17580.1967847300422449No Hit
GTCTATTACTGTGCAAGAGTAAAGTACGCTGGTAACTATTACTTTGACTC17500.19588923639017553No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17450.19532955285763218No Hit
GGCCAAAAGTGTACGGCGAAGTATAATATTGTTGACAGAAATAAACTGCC17070.19107595801030267No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16610.18592686951090376No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15940.17842711017482274No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCTCC15910.17809130005529672No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCTCCCAGAGTATTTTCTA15600.1746212621535279No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15460.1730541482624065No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCTCCCAGAGTATTTTCTACAGCTCCAC15070.1686886167085683No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15050.16846474329555097No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCTCTCTCAC15020.16812893317602495No Hit
CTCCTGTACAGCCTCTGGATTCACCTTCAGCGGCTCCTGGATGCACTGGGTCCGTCAAGTTCCAGAGAAGGGG14960.16745731293697294No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14560.16297984467662605No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14190.1588381865358052No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13740.15380103474291498No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGGTTC13560.15178617402575886No Hit
ATTTTCTACAGCTCCACCAATAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGGTTCTC13400.14999518672162013No Hit
GATTTCTCTCTCACCATTAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAACAATATTATACTTCG12940.14484609822222125No Hit
GTCAGGGACCCCGGATTCCCGGGTGGATGCCCAGAAAAGGAGAACCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA12810.1433909210376085No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGGTGGAGCTG12300.13768214900566625No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12160.1361150351145448No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12090.13533147816898414No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11930.1335404908648454No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCTCTCTCACCATTAGCAGCC11930.1335404908648454No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11910.13331661745182805No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11860.13275693391928467No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11820.13230918709324999No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGGTTCTCCTTTTCTGGGCATCCACCCGGGAATC11820.13230918709324999No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11770.13174950356070664No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11510.12883914919148118No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11400.12760784541988576No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11270.12615266823527305No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCC11270.12615266823527305No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11230.12570492140923836No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11030.12346618727906492No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10990.12301844045303023No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10990.12301844045303023No Hit
GTTTATTTCTGTCAACAATATTATACTTCGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAA10960.12268263033350423No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10910.12212294680096086No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT10890.12189907338794352No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10800.12089164302936549No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG10690.11966033925777009No Hit
GAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTAATGGTGAGAGAGAAATCTGTCCCAGACCCGCTGCCA10630.11898871901871806No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10610.11876484560570072No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCTCCCAGAGTATTTTCTACAGCTCCACCAATAAGAACTA10600.11865290889919204No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10590.11854097219268338No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10540.11798128866014No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10330.11563061782345789No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10110.11316801028026713No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10030.11227251662819776No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9940.1112650862696197No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9930.11115314956311104No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9910.1109292761500937No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9900.11081733944358502No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9810.10980990908500698No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9450.10578018765069479No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG9420.10544437753116878No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9320.10432501046608204No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9310.1042130737595734No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG9310.1042130737595734No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9270.10376532693353871No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG9220.10320564340099533No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9130.1021982130424173No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGGTTCTCCTT9100.10186240292289128No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)