FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893362
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTTCGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCAT81510.9123960947521834No Hit
GTATAATATTGTTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTAATGGTGAGAGAGAAATCTGTC67990.7610576675524591No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41670.46644025602163514No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32740.36648077710939125No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29160.3264074361792868No Hit
CCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGGTGGAGCTGTAGAAAATACT26970.3018932974538877No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCA26170.2929383609331939No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25370.2839834244125002No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25200.2820805004018528No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24240.27133457657702026No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGACTGCTCATTTGCAGATACA22890.2562231211983496No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22230.24883529856877729No Hit
CTCCTAAGGTTCTCCTTTTCTGGGCATCCACCCGGGAATCCGGGGTCCCT21950.24570107078653447No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21860.2446936404279564No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21360.23909680510252282No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21040.23551483049424532No Hit
CCATTAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAACAA20650.23114929894040714No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20430.22868669139721634No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19690.22040337511557462No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18600.2082022741061294No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18540.20753065386707742No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17800.1992473375854357No Hit
GGCCAAAAGTGTACGGCGAAGTATAATATTGTTGACAGAAATAAACTGCC17420.19499374273810616No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17230.1928669453144414No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC17210.19264307190142405No Hit
GTCTATTACTGTGCAAGAGTAAAGTACGCTGGTAACTATTACTTTGACTC17210.19264307190142405No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16580.18559105939137774No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16220.18156133795706556No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15930.17831517346831408No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15740.17618837604464932No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCTCTCTCAC15400.17238252802335446No Hit
CTCCTGTACAGCCTCTGGATTCACCTTCAGCGGCTCCTGGATGCACTGGGTCCGTCAAGTTCCAGAGAAGGGG14820.1658901990458515No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCTCCCAGAGTATTTTCTACAGCTCCAC14570.1630917813831347No Hit
AACCAGGACAGCCTCCTAAGGTTCTCCTTTTCTGGGCATCCACCCGGGAA14560.16297984467662605No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14390.16107692066597862No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13420.15021906013463746No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCTCC13390.14988325001511146No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCTCCCAGAGTATTTTCTA13260.14842807283049872No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT13070.14630127540683396No Hit
GAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTAATGGTGAGAGAGAAATCTGTCCCAGACCCGCTGCCA12880.1441744779831692No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12530.1402566932553657No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12500.13992088313583967No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12470.13958507301631365No Hit
GATTTCTCTCTCACCATTAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAACAATATTATACTTCG12400.13880151607075294No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12280.1374582755926489No Hit
TCTTCAGCCTGCAGGCTGCTAATGGTGAGAGAGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC12050.13488373134294945No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11910.13331661745182805No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11670.13063013649561991No Hit
GTCAGGGACCCCGGATTCCCGGGTGGATGCCCAGAAAAGGAGAACCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA11580.12962270613704188No Hit
GTTTATTTCTGTCAACAATATTATACTTCGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAA11570.1295107694305332No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11540.1291749593110072No Hit
ATTTTCTACAGCTCCACCAATAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGGTTCTC11470.1283914023654465No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGGTGGAGCTG11440.12805559224592045No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11310.12660041506130773No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11260.12604073152876438No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10970.12279456704001289No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10920.12223488350746954No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10910.12212294680096086No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCTCTCTCACCATTAGCAGCC10910.12212294680096086No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10730.12010808608380477No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT10620.11887678231220938No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10570.11831709877966604No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGGTTC10510.11764547854061401No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10280.11507093429091456No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10170.11383963051931915No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10130.11339188369328447No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10000.11193670650867173No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGGTTCTCCTTTTCTGGGCATCCACCCGGGAATC9920.11104121285660236No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG9820.10992184579151565No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9590.1073473015418162No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG9560.10701149142229019No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9550.1068995547157815No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9410.1053324408246601No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9400.10522050411815144No Hit
CTGCTAATGGTGAGAGAGAAATCTGTCCCAGACCCGCTGCCACTGAATCG9370.10488469399862542No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCTCCCAGAGTATTTTCTACAGCTCCACCAATAAGAACTA9280.10387726364004736No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9180.10275789657496065No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9150.10242208645543464No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9140.10231014974892597No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9120.10208627633590862No Hit
GGCTGAAGATGTGGCAGTTTATTTCTGTCAACAATATTATACTTCGCCGTACACTTTTGGCCAGGGGACCAAG9100.10186240292289128No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8970.10040722573827854No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8950.1001833523252612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)