FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004688933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961584
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTCTTCATTTGCAGATATA37980.3949732940647931No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC28370.2950340271884724No Hit
CCCCAGCACTCAGGCTGCTATCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTTCGGTGATGC27540.2864024359806319No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCAGCACTCAGGCTGCTATCCCATGTT27280.28369856403600724No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24660.2564518544401737No Hit
GTATATTACTGTGCGAAAGGTGCCCTTGGCGGGAGCTTCTCTTTGAGTGACTGCTGGGGCCAGGGAACCCTGGT24580.25561989384182765No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCAGCACTCAGG21970.22847717932078737No Hit
GTGCTGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC21280.22130151916005258No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21070.21911762258939416No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20730.2155817900464234No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT20430.21246193780262565No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19640.20424632689395827No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18820.19571873076091117No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18540.19280686866669997No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT17500.18199138088820113No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17340.180327459691509No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTTCGGTGATGCCCAGGGTGG16780.17450373550308657No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16670.17335978968036075No Hit
CTACGTAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCA16390.17044792758614952No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC16110.16753606549193828No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15620.16244030682706867No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15220.15828050383533837No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15170.15776052846137206No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14720.15308075009567548No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14610.15193680427294962No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14600.1518328091981564No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14440.15016888800146425No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAACATCTCCTGCTCTGG14300.14871295695435863No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14030.14590508993494067No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13840.1439291835138688No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG13630.14174528694321037No Hit
GATTATTACTGCGGAACATGGGATAGCAGCCTGAGTGCTGGGGTGTTCGG13620.14164129186841712No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13360.1389374199237924No Hit
GGACAGAAGGTCAACATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGCA13130.13654553320354748No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12780.13290570558578346No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12480.12978585334198572No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG12340.12832992229488013No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC12310.12801793707050033No Hit
GCTCCAACATTGGGCATAATTATGTATGCTGGTACCAGCAGTTCCCAGGA12140.12625002079901496No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG12000.12479408975190935No Hit
GTCTACGTAGTATGTGCTCCCACCACTACCACTAATAGCTGAGACCCACT11880.12354614885439026No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11790.12261019318125094No Hit
GTAATAAGCGATATTCAGGGATTCCCGACCGATTCTCTGGCTCCAAGTCTGGCACGTCCGCCACCCTGGGCATCA11540.12001031631141951No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11440.11897036556348692No Hit
GTCTGGAGTTCGGTGATGCCCAGGGTGGCGGACGTGCCAGACTTGGAGCC11370.1182424000399341No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCAGCACTCAGGC11220.11668247391803524No Hit
GTTTGGGGGCTGTTCCTGGGAACTGCTGGTACCAGCATACATAATTATGC11210.11657847884324199No Hit
GGATAGCAGCCTGAGTGCTGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC11140.1158505133196892No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC11120.11564252317010267No Hit
TAATTATGTATGCTGGTACCAGCAGTTCCCAGGAACAGCCCCCAAACTCC11080.11522654287092964No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11040.1148105625717566No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10990.11429058719779031No Hit
ACCCTGGGCATCACCGAACTCCAGACTGGGGACGAGGCCGATTATTACTG10960.11397860197341055No Hit
ACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAGCA10730.11158671525316562No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10650.11075475465481956No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT10450.1086748531589544No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10370.10784289256060833No Hit
ATGTTGGAGCTGCTTCCAGAGCAGGAGATGTTGACCTTCTGTCCTGGGGC10320.10732291718664204No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAACATCTCCTG10150.10555500091515665No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC10080.10482703539160386No Hit
ATCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTTCGG10080.10482703539160386No Hit
GCCCCCAAACTCCTCATTTATGACAGTAATAAGCGATATTCAGGGATTCCCGACCGATTCTCTGGCTCCAAGT10060.10461904524201734No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAGTAATAAGCGATATTCAGGGATTCCCGACCGATTCTCTGG9970.10368308956887802No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGAGACCAGGGTTCCCTGGCCCCAGCAGTCACTCAAAGAGAAG9930.10326710926970499No Hit
GGGCATCACCGAACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAA9720.10108321269904658No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC9680.10066723239987355No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9670.1005632373250803No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9630.10014725702590727No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)