FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961584
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTCTTCATTTGCAGATATA37870.3938293482420673No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAACATCTCCTG29820.31011331303349476No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC29320.30491355929383185No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25540.2656034210219804No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCAGCACTCAGGCTGCTATCCCATGTT23660.24605234696084793No Hit
GTATATTACTGTGCGAAAGGTGCCCTTGGCGGGAGCTTCTCTTTGAGTGACTGCTGGGGCCAGGGAACCCTGGT23330.24262050949267044No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAA22050.22930913991913343No Hit
GTGCTGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC21880.22754122364764803No Hit
CCCCAGCACTCAGGCTGCTATCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTTCGGTGATGC21550.22410938617947054No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20500.2131899033261785No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20010.20809414466130882No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT19270.20039850912660778No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18810.19561473568611792No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17720.1842792725336528No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT17350.18043145476630226No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17190.17876753356961014No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16940.1761676566997787No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCAGCACTCAGG16690.17356777982994726No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16500.17159187340887536No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16150.16795204579111134No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15660.1628562871262417No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC15300.15911246443368443No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15280.1589044742840979No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG14750.15339273532005523No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14390.14964891262749797No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14290.1486089618795654No Hit
CTACGTAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCA14210.14777700128121932No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13850.14403317858866202No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13690.14236925739196993No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG13360.1389374199237924No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13300.13831344947503288No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAACATCTCCTGCTCTGG13220.13748148887668682No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTTCGGTGATGCCCAGGGTGG12760.13269771543619693No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT12630.1313457794638846No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC12570.13072180901512503No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12510.1300978385663655No Hit
GGACAGAAGGTCAACATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGCA12330.12822592722008685No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12290.12780994692091383No Hit
GATTATTACTGCGGAACATGGGATAGCAGCCTGAGTGCTGGGGTGTTCGG12140.12625002079901496No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11710.12177823258290488No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11550.12011431138621274No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCT11250.11699445914241502No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC11110.1155385280953094No Hit
GTCTACGTAGTATGTGCTCCCACCACTACCACTAATAGCTGAGACCCACT10910.11345862659944425No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC10830.11262666600109819No Hit
GGGCATCACCGAACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAA10730.11158671525316562No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10710.11137872510357909No Hit
GGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGCCCAGT10640.11065075958002629No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10610.11033877435564653No Hit
GTAATAAGCGATATTCAGGGATTCCCGACCGATTCTCTGGCTCCAAGTCTGGCACGTCCGCCACCCTGGGCATCA10470.10888284330854091No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10470.10888284330854091No Hit
ACCCTGGGCATCACCGAACTCCAGACTGGGGACGAGGCCGATTATTACTG10430.10846686300936788No Hit
TAATTATGTATGCTGGTACCAGCAGTTCCCAGGAACAGCCCCCAAACTCC10380.1079468876354016No Hit
GCTCCAACATTGGGCATAATTATGTATGCTGGTACCAGCAGTTCCCAGGA10140.10545100584036339No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG10070.1047230403168106No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10030.10430706001763757No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9880.1027471338957387No Hit
GCCCCCAAACTCCTCATTTATGACAGTAATAAGCGATATTCAGGGATTCCCGACCGATTCTCTGGCTCCAAGT9840.10233115359656567No Hit
GGGTGGCGGACGTGCCAGACTTGGAGCCAGAGAATCGGTCGGGAATCCCT9790.10181117822259937No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA9790.10181117822259937No Hit
ATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTG9710.10097921762425331No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9650.10035524717549377No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)