FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1389940
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC63220.4548397772565722No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60730.4369253349065428No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACGTGGGCTTGAGTGGATGGGATGGATCCACCCTAATAGTGGCGG56660.4076434954026793No Hit
GGGTTACACTACCACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT54250.3903046174655021No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45540.32764004201620217No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40910.29432925162237217No Hit
GTGTAACCCTGTTGACAAAAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGATGAGAGTGAAGTCTGTC38260.275263680446638No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38230.27504784379181835No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37660.2709469473502453No Hit
GTGTATATAGTGGGTGATGAAGGTGTATCCAGAGGCCTTGCAGGAGACCTTCACTGAGGCCCCAGGCTTCTTCAC35850.2579248025094608No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35610.2561981092709038No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG35300.2539677971711009No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34200.24605378649438106No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34090.24526238542670908No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG33240.23914701354015283No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33100.23813977581766121No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32990.23734837474998924No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31100.22375066549635236No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30930.2225275911190411No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG26830.193029914960358No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26040.187346216383441No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25240.18159057225491748No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC25010.17993582456796695No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24500.1762666014360332No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG24030.17288516051052563No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23970.17245348720088638No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23890.17187792278803402No Hit
ATGCTACATCCACTTTGCAAAGTGGCGTCCCATCAAGGTTCAGTGGCACT23590.1697195562398377No Hit
CCCTAATAGTGGCGGCACATACTATGCACAGAAATTTCAGGGCAGAGTCACCATTACCAGGGACACGTCCACCAG23480.1689281551721657No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23300.1676331352432479No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22990.16540282314344504No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC22010.15835215908600372No Hit
CTCCTGATCTATGCTACATCCACTTTGCAAAGTGGCGTCCCATCAAGGTTCAGTGGCACTAGATCTGGGACAGA21890.15748881246672516No Hit
AGTCAGAACATTGACAGTTTTTTGAATTGGTATCAGCAGAAACCAGGGGA21230.15274040606069328No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21130.1520209505446278No Hit
GTCTGCATCTGTGGGAGACAGAGTCAGCATCACTTGCCGGGCAAGTCAGA21110.15187705944141475No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20960.15079787616731657No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20930.15058203951249693No Hit
GATCTATGCTACATCCACTTTGCAAAGTGGCGTCCCATCAAGGTTCAGTGGCACTAGATCTGGGACAGACTTCA20900.15036620285767732No Hit
GTCTCCATCTTCTCTGTCTGCATCTGTGGGAGACAGAGTCAGCATCACTT20440.14705670748377628No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19570.14079744449400694No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19250.1384951868425975No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19230.13835129573938443No Hit
GAGTGAAGTCTGTCCCAGATCTAGTGCCACTGAACCTTGATGGGACGCCA19140.13770378577492554No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19120.13755989467171245No Hit
GATCAGGAGCTTGGGGGCTTCCCCTGGTTTCTGCTGATACCAATTCAAAA19080.13727211246528626No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18950.1363368202944012No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCTTCT18900.13597709253636847No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18670.13432234484941796No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC18610.13389067153977868No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18440.13266759716246745No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18320.13180425054318892No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT17880.12863864627250096No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17820.1282069729628617No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17800.12806308185964863No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17760.12777529965322243No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT17660.12705584413715698No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17630.12684000748233737No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17340.1247535864857476No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG17210.12381829431486252No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17050.12266716548915779No Hit
ATCTGGGACAGACTTCACTCTCATCATCAGCAGTCTGCAACCTGAAGATT17050.12266716548915779No Hit
GACTTCACTCTCATCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA16730.12036490783774839No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16710.12022101673453531No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16690.12007712563132222No Hit
GTAATAAAAGGCCGTGTCGTCAGATTCCAGCCTGTTCAGCTCCATGTAGG16510.11878210570240443No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16450.11835043239276516No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16440.1182784868411586No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16340.11755903132509317No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16140.11612012029296229No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16050.1154726103285034No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15800.11367397153833977No Hit
GTCCCAGATCTAGTGCCACTGAACCTTGATGGGACGCCACTTTGCAAAGT15720.11309840712548742No Hit
GTGGGAGACAGAGTCAGCATCACTTGCCGGGCAAGTCAGAACATTGACAGTTTTTTGAATTGGTATCAGCAGAAA15640.11252284271263509No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15550.11187533274817618No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15490.11144365943853692No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15230.10957307509676677No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14810.10655136192929192No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT14630.10525634200037412No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC14620.10518439644876756No Hit
CTATTAGGGTGGATCCATCCCATCCACTCAAGCCCACGTCCAGGGGCCTG14480.10417715872627596No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14390.10352964876181707No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14350.10324186655539089No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG14260.102594356590932No Hit
GTAGTGTAACCCTGTTGACAAAAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGATGAGAGTGAAGT14030.10093960890398146No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA200.00718420852.06614744
CTTCGAT200.007593996451.34076337
ATATCGT200.007593996451.34076337
TGTCGTA507.307972E-847.9898138
TCGTATG3850.045.60602640
GTGGTAT2550.045.035091
CGTATGC3950.044.54786341
GTCGTAT551.518656E-743.72772639
AGACGTA4700.042.441325
CGAGACG4750.041.9673423
CGTAGAG4800.041.6381128
ACGTAGA4800.041.60357327
GAGACGT4800.041.5391524
ACGAGAC4800.041.51672722
GCCGTCT3950.041.4485946
ATGCCGT4300.041.16858344
GACGTAG4850.041.1509226
CTTATAC4800.040.8130531
TGCCGTC4300.040.43763745
CACGAGA5050.040.1302821
Produced by FastQC (version 0.11.5)