FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1370864
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATACA56570.41265946147830856No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATAAC43150.31476499492290994No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42510.31009640635394903No Hit
GATTATTACTGCTCCTCATTTGCAGGCAGTAGCACTTTCGTTATATTCGG40680.2967471609145765No Hit
GTATATTACTGTGCGAAAGACTCCCCCTCACTAGGACATTGTAGTGGTGG38480.28069888770877344No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33700.2458303668343468No Hit
GGACTGAACAGAGAGAACTCACCATGGAATTTGGGCTGAGCTGGCTTTTT32840.23955695094480559No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG31540.23007388041410382No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31300.22832315970074346No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC30480.22234153059676234No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATAACGAAAGTGCTA29070.2120560464057704No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26780.19535125293245723No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26780.19535125293245723No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG25590.18667059606204553No Hit
CTCCTGCACTGGATCCAGCAGTGATGTTGGAATTTATAACCTTGTGTCCTGGTACCAACATCACCCAGGCAAAGC24720.18032423347611434No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24440.17828172597719397No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGATCCAGCAGTGATGTT24340.1775522590132938No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23630.1723730435696028No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23490.17135178982014262No Hit
CACTAGGACATTGTAGTGGTGGTAGCTGCTACTCCACTGCTTTTGATACC23110.1685798153573221No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22730.16580784089450157No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22480.16398417348475122No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21840.15931558491579031No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21310.1554494100071196No Hit
GTCCTAGTGAGGGGGAGTCTTTCGCACAGTAATATACGGCCGTGTCCTCG21110.15399047607931932No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC20770.15151028840205885No Hit
GTCGATCACCATCTCCTGCACTGGATCCAGCAGTGATGTTGGAATTTATAACCTTGTGTCCTGGTACCAACATCA20480.14939483420674846No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGTCATGCCATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG20470.14932188751035844No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20420.14895715402840837No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20080.1464769663511479No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19940.14545571260168771No Hit
CCCCTGAGGGCCGCCTAGTGCCCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGATGTTGGTACCAGGACA19860.1448721390305676No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19830.14465329894139753No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19670.14348615179915733No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19450.14188132447857701No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19440.141808377782187No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19240.14034944385438672No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19180.13991176367604666No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT19140.13961997689048658No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19110.13940113680131655No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT18890.13779630948073623No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18880.13772336278434621No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18840.13743157599878617No Hit
CAGTAGCACTTTCGTTATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT18530.13517022841069573No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18450.13458665483957563No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18340.13378424117928547No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18090.13196057376953513No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGTCATGCCATGAATTGGGTCCG18030.13152289359119504No Hit
GGATCCAGCAGTGATGTTGGAATTTATAACCTTGTGTCCTGGTACCAACA17800.12984511957422473No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT17580.12824029225364442No Hit
GTGCTACTGCCTGCAAATGAGGAGCAGTAATAATCAGCCTCGTCCTCAGC17480.12751082528974428No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17470.12743787859335426No Hit
CCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGG17400.12692725171862415No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17000.12400938386302361No Hit
GGCTTGGGACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGG16830.12276929002439337No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16780.1224045565424433No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC16540.12065383582908298No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16520.12050794243630294No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCACAGTCCCTTGG16450.11999731556157284No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16190.1181007014554325No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATA16150.11780891466987244No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16120.11759007458070239No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16060.1171523944023623No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16020.11686060761680224No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15970.11649587413485218No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15950.11634998074207216No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC15770.11503694020705191No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15760.11496399351066189No Hit
CCTTTAGCAGTCATGCCATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG15650.11416157985037173No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC15600.11379684636842168No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATAACGAAAGTGCTAC15420.11248380583340142No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC15380.11219201904784136No Hit
GTGTTAATACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCC15290.11153549878033123No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15230.11109781860199117No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG15130.11036835163809101No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAATTTGGGCTGAGCTGGC15110.11022245824531098No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT15100.11014951154892097No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14750.10759637717527049No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14540.10606449655108019No Hit
GGCAAAGCCCCCAAACTCATGATTTATGAGGGCACTAGGCGGCCCTCAGG14360.10475145601605994No Hit
GAACAGAGAGAACTCACCATGGAATTTGGGCTGAGCTGGCTTTTTCTTGT14320.1044596692304999No Hit
AATACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGGTGTATCTT14200.10358430887381972No Hit
GCTTGGTCCCTCCGCCGAATATAACGAAAGTGCTACTGCCTGCAAATGAGGAGCAGTAATAATCAGCCTCGTCCT14180.10343841548103969No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14100.10285484190991959No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13750.1003017075362691No Hit
GTATTAACACCACTACCACTAACAGTTGAGACCCACTCCAGCCCCTTCCC13730.10015581414348908No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACCG303.7417744E-451.14486368
TTACTAG400.00879716749.46370
TTACCGG354.7471168E-448.7358469
GTCGTAT950.047.16638639
AGGGGGG3150.046.06078370
TGTCGTA1100.043.81147838
TCTGGAG17200.042.9446270
ATGTCGT1100.040.63119537
GTGGTAT2750.039.6747471
TCGTATG2750.038.90333640
CGTATGC2900.038.12478341
GACAGGT1700.038.106721
TGAGCGA3150.036.90921431
GTATGCC3100.036.83139842
ACGAGAC3300.036.15396522
CCCACGA3400.035.08677319
CGAGCCC3500.035.05813215
TACACAT3600.035.037475
ATGCCGT3300.034.68395244
TGGTATC3050.034.650642
Produced by FastQC (version 0.11.5)