FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1370864
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATACA52380.3820947956908927No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC41300.30126985609075735No Hit
GATTATTACTGCTCCTCATTTGCAGGCAGTAGCACTTTCGTTATATTCGG38710.28237666172574377No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37930.27668681940732265No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATAAC37670.2747902053011823No Hit
GTATATTACTGTGCGAAAGACTCCCCCTCACTAGGACATTGTAGTGGTGG35480.2588148787917693No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32730.23875453728451546No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32600.2378062302314453No Hit
GGACTGAACAGAGAGAACTCACCATGGAATTTGGGCTGAGCTGGCTTTTT31800.23197049452024415No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG28490.20782513801514954No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC27130.19790438730610768No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG25630.18696238284760558No Hit
CTCCTGCACTGGATCCAGCAGTGATGTTGGAATTTATAACCTTGTGTCCTGGTACCAACATCACCCAGGCAAAGC24920.18178316740391462No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGATCCAGCAGTGATGTT24770.1806889669580644No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATAACGAAAGTGCTA24700.1801783400833343No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23840.1739049241937931No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23690.1728107237479429No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22050.1608474655399806No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21940.16004505187969048No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC21790.15895085143384027No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21660.15800254438077008No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21590.15749191750604No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGTCATGCCATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG21480.15668950384574984No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21170.1544281562576594No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20950.1528233289370791No Hit
GTCCTAGTGAGGGGGAGTCTTTCGCACAGTAATATACGGCCGTGTCCTCG20810.1518020751876189No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20630.15048903465259866No Hit
GTCGATCACCATCTCCTGCACTGGATCCAGCAGTGATGTTGGAATTTATAACCTTGTGTCCTGGTACCAACATCA20480.14939483420674846No Hit
CACTAGGACATTGTAGTGGTGGTAGCTGCTACTCCACTGCTTTTGATACC20100.1466228597439279No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19920.14530981920890768No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19470.14202721787135705No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19410.14158953769301694No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18890.13779630948073623No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18760.13684800242766607No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18730.136629162338496No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18550.13531612180347577No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18340.13378424117928547No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18310.13356540109011542No Hit
CCCCTGAGGGCCGCCTAGTGCCCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGATGTTGGTACCAGGACA18140.1323253072514852No Hit
CAGTAGCACTTTCGTTATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT18020.13144994689480502No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT17900.13057458653812484No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC17670.12889681252115454No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17640.1286779724319845No Hit
GGATCCAGCAGTGATGTTGGAATTTATAACCTTGTGTCCTGGTACCAACA17510.12772966537891434No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17400.12692725171862415No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17150.1251035843088738No Hit
CCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGG17120.12488474421970379No Hit
GTGCTACTGCCTGCAAATGAGGAGCAGTAATAATCAGCCTCGTCCTCAGC17040.12430117064858366No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16910.12335286359551349No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16740.12211276975688326No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16710.12189392966771322No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGTCATGCCATGAATTGGGTCCG16580.12094562261464303No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16370.11941374199045274No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC16210.11824659484821251No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16200.1181736481518225No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC15870.11576640717095203No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15710.11459926002871182No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT15690.11445336663593178No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15500.11306737940452152No Hit
GGCTTGGGACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGG15440.11262969922618145No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15430.11255675252979144No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15370.11211907235145135No Hit
CCTTTAGCAGTCATGCCATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG15160.11058719172726106No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15110.11022245824531098No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15060.10985772476336092No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14950.10905531110307076No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14950.10905531110307076No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG14870.10847173753195066No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCACAGTCCCTTGG14590.10642923003303026No Hit
GTGTTAATACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCC14590.10642923003303026No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14220.10373020226659975No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14170.10336546878464967No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATA13940.10168769476767937No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)