FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405365
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCGCCAAGAATAAGAACTACTTAAGTTGGTACCAGCAGAA85090.6054654840557435No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT66610.4739693958508999No Hit
ATATTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT65120.463367167959925No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAAGCCGGGGTCCCT54930.3908593141283581No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48810.34731190829428654No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46690.3322268592145101No Hit
CTATAATATTGGTGACAGTAATAAACTGCCACATCTCCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC44510.31671487478341925No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39850.2835562291646654No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36660.26085749965311505No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35880.25530734008602746No Hit
ATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA35850.2550938724103703No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAACTTAAGTAGTTCTTATTCTTGGCGCTGTATAAAACACT34910.2484052185731109No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34570.2459859182489958No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33500.2383722378172219No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31270.22250447392670233No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA30450.21666969079207182No Hit
GAGTACTATAATATTGGTGACAGTAATAAACTGCCACATCTCCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT30340.2158869759813287No Hit
GTTTATTACTGTCACCAATATTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA30060.21389461100852802No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA29460.20962525749538374No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28720.20435972149583917No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA28560.20322122722566735No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28150.20030383565835208No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27280.1941132730642929No Hit
TTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT27260.19397096128052144No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27070.1926189993346924No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26660.18970160776737716No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26390.18778039868646224No Hit
GTAATAAACTGCCACATCTCCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA25950.18464953944348977No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25670.1826571744706891No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25560.181874459659946No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25340.18030903003845977No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA25080.17845897684943057No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24850.17682239133605862No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24360.17333575263365744No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24200.17219725836348565No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC23880.16992026982314204No Hit
GAATAAGAACTACTTAAGTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTAC23230.16529513685056907No Hit
GTACTATAATATTGGTGACAGTAATAAACTGCCACATCTCCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT22890.162875836526454No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA21950.15618718268919463No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21900.15583140322976594No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA21890.15576024733788021No Hit
GGCCAAAAGTGTACGGAGTACTATAATATTGGTGACAGTAATAAACTGCC21830.15533331198656575No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG21760.1548352207433656No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20030.14252525144713296No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC19460.13846936560964587No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGGAGATGTGGCAGTTTATTACTGTCACCAATATTATAGTACT19450.13839820971776018No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19390.1379712743664457No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19360.13775780669078852No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19160.13633468885307376No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18940.13476925923158753No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18940.13476925923158753No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18230.12971719090770015No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT18070.12857869663752833No Hit
GCTCATTTACTGGGCATCTACCCGGGAAGCCGGGGTCCCTGCCCGATTCAGTGGCAGCGGGTCTGGGACAGATTT17990.12800944950244242No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA17910.12744020236735654No Hit
TCTCCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGGCAGGGACC17750.12630170809718472No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC17490.12445165490815553No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17310.12317084885421226No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17230.12260160171912635No Hit
GTCCCAGACCCGCTGCCACTGAATCGGGCAGGGACCCCGGCTTCCCGGGT17010.1210361720976401No Hit
GGCTGGAGATGTGGCAGTTTATTACTGTCACCAATATTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAG16600.11811878053032486No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16310.11605525966563847No Hit
GTCCCTGCCCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC16100.11456098593603796No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGGCAGGGACCCCG15980.11370711523340912No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15700.11171475026060845No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15650.11135897080117978No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15640.11128781490929403No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC15640.11128781490929403No Hit
GTGTATTACTGTGCGAGAGACAAATACTATGAAAGTAGAGGTTATATCCC15290.10879735869329321No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15240.10844157923386451No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15160.1078723320987786No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15080.1073030849636927No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15030.10694730550426401No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14920.1061645906935209No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14860.10573765534220647No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14810.1053818758827778No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT14480.10303373145054842No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14370.10225101663980532No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14320.10189523718037662No Hit
CACCATCAGCAGCCTGCAGGCTGGAGATGTGGCAGTTTATTACTGTCACC14250.10139714593717646No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCGCCAAGAATAAGAACTA14180.1008990546939763No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACC200.007678024551.198122
GGTATCA14300.047.9997831
TGGTATC3750.042.778872
GTGGTAT3850.042.57551
GTATCAA38650.030.6590861
GTGCGAA450.00487872930.330999
TCGCTCG703.5417797E-529.93701250
TATCAAC40000.029.5242482
ATCAACG40700.028.9223023
TCAACGC41100.028.8068544
GACAGGT2500.028.6852421
AACGCAG41900.028.5031666
CAACGCA41500.028.4490055
ACAGACG6800.028.06663770
CGCCCAG9500.027.77931445
TGTTAGA14250.027.71007370
GGGTACG500.00815233427.3046918
ACGCAGA43750.027.297897
CGCAGAG43750.027.2198968
CGTTGTC9300.026.9082163
Produced by FastQC (version 0.11.5)