FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772040
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34340.4447956064452619No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGTGCTGCTGCTTGTATATGAACTG27890.36125071239832135No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG26400.34195119423864045No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24650.3192839749235791No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23670.3065903321071447No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGTGCTGCTGCTTGTATATGAACTGCAGTAATAAT23310.3019273612766178No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACCGGAACCAGCAGTGACGTT22540.2919537847779908No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22320.2891041914926688No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20630.2672141339826952No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19920.25801771928915596No Hit
GTAATACACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCTTCAGGGAGA19100.2473965079529558No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17860.23133516398114087No Hit
GTCGATCACCATCTCCTGCACCGGAACCAGCAGTGACGTTGGTGGTTATAACTTTGTCTCCTGGTACCAACAACA17670.22887415159836277No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC16990.2200663178073675No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGTTCATAT16530.21410807730169423No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16340.21164706491891613No Hit
GTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG15800.20465260867312574No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15770.20426402777058184No Hit
CTCCTGCACCGGAACCAGCAGTGACGTTGGTGGTTATAACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC15520.2010258535827159No Hit
GATTATTACTGCAGTTCATATACAAGCAGCAGCACTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT14970.19390187036941092No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14950.19364281643438164No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14810.19182943888917672No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14740.19092275011657428No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14600.18910937257136934No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT14520.18807315683125228No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14200.18392829387078388No Hit
GAATACCACAGTGCTGCTGCTTGTATATGAACTGCAGTAATAATCAGCCT14100.1826330241956375No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14050.18198538935806435No Hit
GTCTCTGGTGGCTCCATCTACAGGGGTGATCACTACTGGGGCTGGATCCG13970.18094917361794724No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13510.17499093311227398No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13440.1740842443396715No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13380.1733070825345837No Hit
CTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGCCTGGAGTGGATTGGGTACATGTATTACAGTGGGACCAC13300.1722708667944666No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13000.1683850577690275No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12960.16786694989896897No Hit
CAGCAGCACTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA12890.16696026112636653No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12700.16449924874358843No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12650.16385161390601524No Hit
GTGTATTACTGTGCCAGTGCCAACGTGGATACAACTATGGTTATTTCTTACTTTGACTACTGGGGCCAGGGAACC12580.16294492513341277No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC12120.15698668462773951No Hit
GGTTATAACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTTTGAGGTCAGTGAT11900.1541370913424175No Hit
GTGCTGCTGCTTGTATATGAACTGCAGTAATAATCAGCCTCGTCCTCAGC11880.1538780374073882No Hit
GTATTACAGTGGGACCACCTACTACAACCCGTCCCTCAAGAGTCGAGTTA11730.15193513289466867No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11680.1512874980570955No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11410.1477902699342003No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11330.14675405419408322No Hit
CCACAGTGCTGCTGCTTGTATATGAACTGCAGTAATAATCAGCCTCGTCC11160.1445520957463344No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC10970.1420910833635563No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10890.1410548676234392No Hit
CTACAGGGGTGATCACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGG10820.14014817885083675No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10770.13950054401326356No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGTGCTGCTGC10670.1382052743381172No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC10510.13613284285788302No Hit
GCACTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC10400.134708046215222No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG10260.1328946686700171No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10210.1322470338324439No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC10160.13159939899487072No Hit
GCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG9920.12849075177451946No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9860.12771358996943163No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9780.12667737422931455No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC9740.126159266359256No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9710.12577068545671208No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG9690.12551163152168282No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9600.12434588881405108No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGAT9390.12162582249624371No Hit
GTTCATATACAAGCAGCAGCACTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTG9260.11994197191855345No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9250.1198124449510388No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCAGTAGACACGTCCAAGAATCAGTTCTCCCTGAAG9200.11916481011346562No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8920.11553805502305581No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT8850.11463136625045334No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8840.11450183928293871No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8780.1137246774778509No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC8690.11255893477021917No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC8600.11139319206258742No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8570.11100461116004352No Hit
GCTTGGTCCCTCCGCCGAATACCACAGTGCTGCTGCTTGTATATGAACTGCAGTAATAATCAGCCTCGTCCTCAG8550.11074555722501425No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8500.11009792238744108No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC8300.10750738303714834No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8100.1049168436868556No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8020.10388062794673852No Hit
CAGTAATACACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCTTCAGGGAGAACTGATTCTTGGACGTGTCTA8010.10375110097922387No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC7970.10323299310916532No Hit
CCCCTGAGGGCCGATCACTGACCTCAAAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA7970.10323299310916532No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGTGCTG7950.10297393917413605No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA7940.10284441220662142No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC7910.1024558313040775No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTTTGAGGTCAGTGATCGGCCCTCAGGGGTTTCTAATCG7860.10180819646650432No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7850.1016786694989897No Hit
CTGTAATACATGTACCCAATCCACTCCAGGCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGTAGTGATCACCC7810.10116056162893115No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7810.10116056162893115No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT7800.10103103466141651No Hit
GTATATGAACTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA7800.10103103466141651No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACTA150.002517422767.85095
GCCTAGT200.006850606352.6936651
TCGTATG850.052.5945540
TCTCGAG200.00786322450.88817215
TATCCGG200.00786322450.8881725
ATGCCGT950.047.4476844
GTATGCC1000.044.88809642
CGTATGC1000.044.8024441
GCCGTCT1050.043.0771446
CCCACGA1050.042.00021419
TCTCGTA759.331416E-1041.1126438
GGTATCA5200.040.4548151
AGACCGA1100.040.10930325
ACGAGAC1100.040.0963122
TTATACA1100.040.093712
CGAGCCC1100.040.0937115
TATACAC1100.040.093713
CTTATAC1200.039.5848161
GACAGGT955.456968E-1239.2871861
GTATCAA13150.038.7032431
Produced by FastQC (version 0.11.5)