FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77773
Sequences flagged as poor quality0
Sequence length20-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT19832.549728054723362No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC3910.5027451686317874No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3510.4513134378254664No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2860.36773687526519483No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.30730459156776774No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.30344721175729367No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.2931608655960295No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2150.27644555308397517No Hit
GAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGT2030.2610160338420789No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2010.2584444473017628No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.24944389441065667No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.2404433415195505No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.2365859617090764No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.22629961554781222No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.21729906265670604No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.2044411299551258No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT1580.2031553366849678No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG1560.20058375014465174No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1490.19158319725354558No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTCTGCGTTGATAC1490.19158319725354558No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.19029740398338754No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1430.18386843763259741No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.18258264436243943No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.17358209147133324No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.17358209147133324No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.17358209147133324No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGG1340.1722962982011752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.1658673318503851No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.16458153858022706No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.162009952039911No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.160724158769753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.1555809856891209No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1200.1542951924189629No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.15172360587864683No Hit
GAGTACGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTT1160.1491520193383308No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.13757987990690856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1060.13629408663675055No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.13243670682627648No Hit
GAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT1030.13243670682627648No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT1030.13243670682627648RNA PCR Primer, Index 46 (95% over 21bp)
GTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTA1010.12986512028596042No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.12857932701580238No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGG990.1272935337456444No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTC980.12600774047548635No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT980.12600774047548635No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.12343615393517031No Hit
GTATCAACGCAGAGTACGGGGATCGAAAGATTTCCGCATCCCCGAAAGGG870.11186401450374808No Hit
GGTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT850.10929242796343204No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTATGCCGT840.10800663469327401No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.10543504815295796No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.10414925488279995No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.10414925488279995No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.10286346161264193No Hit
GGTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAG800.10286346161264193No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGT780.10029187507232587No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT780.10029187507232587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2850.0116.0651870
GCGTGAA353.8016879E-1093.0191356
AAGGGGG2900.087.9973869
GCTTCTG159.896706E-485.7306550
CTCTTCT150.001040752484.652849
GGCTTCT150.001040752484.652849
CGGTAAA150.001170492182.1887347
AATAGGA150.00133589279.5013645
TATGGCG205.0829512E-576.4462943
TGAAAAA4050.074.7757159
GTTTGGC150.001724144974.5579242
CGTGAAA452.406523E-973.6792957
CGTATGG252.1664055E-673.1253141
TTGAAAA3500.072.58868458
AAATCGG200.00208481971.0478957
CTGCGTG453.3414835E-970.695454
AAAGGGG3100.070.68503668
GCGTCTT359.622272E-870.4474947
TGAAGTC150.002170079470.3625439
CTCGGAT150.002170079470.3625439
Produced by FastQC (version 0.11.5)