FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77773
Sequences flagged as poor quality0
Sequence length20-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45795.887647384053592No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.44745605801499233No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.4448844714746763No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.375451634886143No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2530.32530569734998005No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2470.31759093772903196No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.30987617810808377No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.2957324521363455No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.27387396654365914No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2100.27001658673318507No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.25715865403160476No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.24430072133002456No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.2404433415195505No Hit
GAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGT1850.23787175497923443No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.22115644246718014No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1700.21858485592686408No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.2082985097655999No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.2082985097655999No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTCTGCGTTGATAC1560.20058375014465174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.18644002417291347No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT1450.18644002417291347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.18515423090275546No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1440.18515423090275546No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.18129685109228139No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.16972471166085917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.16843891839070116No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.16715312512054312No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.16329574531006905No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1260.162009952039911No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.15815257222943696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.15686677895927892No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.15172360587864683No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.14658043279801475No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.1440088462576987No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGG1120.1440088462576987No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTT1090.14015146644722462No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1090.14015146644722462No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTC1030.13243670682627648No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.13115091355611844No Hit
GAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT1010.12986512028596042No Hit
CCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTCTGCGTTGATAC980.12600774047548635No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.12215036066501228No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG950.12215036066501228No Hit
GAGTACGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTT910.11700718758438017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT910.11700718758438017No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG900.11572139431422217No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTCTGCGTTGATACC860.11057822123359007No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT840.10800663469327401No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.10414925488279995No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT800.10286346161264193No Hit
GTATCAACGCAGAGTACGGGGATCGAAAGATTTCCGCATCCCCGAAAGGG800.10286346161264193No Hit
CCCGAAAGGGTACATGGCGTTAGGTCGCAAAAAAAAAAAAAAAAAAAAAA780.10029187507232587No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTT780.10029187507232587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGACC156.2283076E-496.3136461
ATGGCCA156.8638125E-493.9916160
CACACTG200.001498120177.2112665
TCAGCGT200.001721564974.55765563
CTGTCTC358.206362E-569.33539669
AACGATG200.002708489366.5192956
ACTGTCT351.2546699E-463.65075768
CCACACT250.003883783260.7232564
GCCACAC250.00416954959.64612263
TGGCCAC250.004727058657.7881961
GCTTGTG150.004767249357.7014122
GCAAGGC150.00477941657.6643073
CATGGAC250.005207251856.3949760
CGGTGGG300.006654322652.9952767
TAACGAT250.00692033652.48858655
ACACTGT300.007003741352.31505266
GCTAAAA300.00895970849.16008862
GTTCCGA200.00956361848.38201533
CTAACGC950.045.5244523
GAATGGC356.748838E-445.3040347
Produced by FastQC (version 0.11.5)