FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018486
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42260.41492961120722327No Hit
CTATAGGAGAGCAAGCAAAAATAGTCAGCCTCATCTTCAGGCAGCGCACCCGAAAGGGTCAGGGCAGCTTTGTCC39490.3877323792374171No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33050.32450126953144176No Hit
CTCCTATAGAGGTGCTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCC32300.3171373980594726No Hit
CCTATAGAGGTGCTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTA30760.30201691530369584No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC30350.2979913322323527No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29240.28709280245383834No Hit
GTCCAGGAGTGTCTGGTGCTTGCATCATAAATCAGTGTCCTGGGGGCTTG27430.2693213259681527No Hit
CTCCTGGACGCCTGCCCGGTTCTCAGGCTACCTCCTTGGGGACAAAGCTGCCCTGACCCTTTCGGGTGCGCTGC26220.25744094666004247No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26160.2568518369422849No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25570.25105892471766916No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAGCACCTCTATAGGAGAGCAAGCAAAAATAGTCAG24940.24487327268121506No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA24090.23652755167964998No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22830.22415624760674177No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22110.21708693099365137No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21240.20854484008616714No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20880.2050101817796219No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20830.20451925701482399No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20710.2033410375793089No Hit
CCTCTATAGGAGAGCAAGCAAAAATAGTCAGCCTCATCTTCAGGCAGCGC20320.1995118244138849No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT19970.1960753510602993No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18990.18645322567025957No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18930.18586411595250205No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18850.18507863632882532No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18760.18419497175218902No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18410.18075849839860342No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17720.17398373664439176No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17670.17349281187959384No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16940.16632531031354383No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16860.1655398306898671No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCC16850.16544164573690753No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16680.1637725015365945No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16150.1585686990297363No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACTTGTGGCTCCA15430.1514993824166459No Hit
TCTTCAGGCAGCGCACCCGAAAGGGTCAGGGCAGCTTTGTCCCCAAGGAGGTAGCCTGAGAACCGGGCAGGCGTC15090.14816109401601985No Hit
GCCTGAAGATGAGGCTGACTATTTTTGCTTGCTCTCCTATAGAGGTGCTGTGGTTTTCGGCGGAGGGACCAAG14970.1469828745805048No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14390.14128814730884864No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14190.13932444824965684No Hit
GTTCTCAGGCTACCTCCTTGGGGACAAAGCTGCCCTGACCCTTTCGGGTG13370.13127328210697053No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACTTGTGGCTCCAGC13360.13117509715401096No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13250.13009506267145549No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13140.12901502818889998No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13080.12842591847114246No Hit
GATTTATGATGCAAGCACCAGACACTCCTGGACGCCTGCCCGGTTCTCAGGCTACCTCCTTGGGGACAAAGCTGC13050.12813136361226368No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12980.12744406894154656No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12900.12665858931786986No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12670.1244003353997993No Hit
GGGCAGCTTTGTCCCCAAGGAGGTAGCCTGAGAACCGGGCAGGCGTCCAG12610.12381122568204178No Hit
GCACTGGAGCTGTCACCGATGGTCATTATCCCTACTGGTTCCAGCAGAAG12450.12224026643468835No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12430.12204389652876917No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC11990.11772375859854727No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAGCACCTCTATAGGAGAGCAAGCAAAAATAGTCAGC11940.1172328338337493No Hit
GTCATTATCCCTACTGGTTCCAGCAGAAGCCTGGCCAAGCCCCCAGGACA11830.11615279935119384No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11710.11497457991567876No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11650.11438547019792122No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGC11600.11389454543312327No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAG11420.11212721627985069No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11370.11163629151505272No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11170.10967259245586096No Hit
CCCGAAAGGGTCAGGGCAGCTTTGTCCCCAAGGAGGTAGCCTGAGAACCGGGCAGGCGTCCAGGAGTGTCTGGTG10890.10692341377299246No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10870.1067270438670733No Hit
GAGGTAGCCTGAGAACCGGGCAGGCGTCCAGGAGTGTCTGGTGCTTGCAT10850.10653067396115411No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10830.10633430405523492No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10770.1057451943374774No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC10680.1048615297608411No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10680.1048615297608411No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10490.10299601565460889No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10450.10260327584277054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAT2800.048.5096970
GGTATCA12900.041.3404921
CGTATGC1350.040.9579341
ACCGTAC1400.038.8908527
TCTCGTA1350.038.19767438
ATCTCGT1350.038.1389137
TAACGGC557.734499E-637.38981236
GTATCAA34200.037.0466041
TCGTATG1500.036.7996340
GACCGTA1500.036.27136226
CTAACGA2800.035.5016669
ACTAGAT1650.035.188832
TATCAAC37150.034.099792
GTACTAG1700.034.0932330
GTGGTAT3600.033.9676631
ATCAACG38150.033.294983
GTACGTA652.3131222E-433.24570569
TCAACGC38350.033.121344
CTAGATC1550.033.07169733
CAACGCA38600.032.994815
Produced by FastQC (version 0.11.5)