FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018486
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC41390.406387520299739No Hit
CTCCTGGACGCCTGCCCGGTTCTCAGGCTACCTCCTTGGGGACAAAGCTGCCCTGACCCTTTCGGGTGCGCTGC36430.3576877836317829No Hit
CTATAGGAGAGCAAGCAAAAATAGTCAGCCTCATCTTCAGGCAGCGCACCCGAAAGGGTCAGGGCAGCTTTGTCC34310.33687257360434997No Hit
CTCCTATAGAGGTGCTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCC31970.31389729461180615No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30320.29769677737347394No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29770.29229660496069654No Hit
CCTATAGAGGTGCTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTA28800.28277266452361643No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC26470.2598955704840322No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26130.25655728208340617No Hit
GTCCAGGAGTGTCTGGTGCTTGCATCATAAATCAGTGTCCTGGGGGCTTG25760.25292443882390137No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24780.24330231343386166No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA24710.24261501876314454No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23290.22867275544288285No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22160.21757785575844932No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21540.2114903886749548No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21190.2080539153213692No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT20900.20520655168554108No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAGCACCTCTATAGGAGAGCAAGCAAAAATAGTCAG19940.19578079620142055No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19880.195191686483663No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19390.19038062378864312No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17930.17604562065654314No Hit
CCTCTATAGGAGAGCAAGCAAAAATAGTCAGCCTCATCTTCAGGCAGCGC17630.17310007206775546No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC17390.17074363319672534No Hit
GTTCTCAGGCTACCTCCTTGGGGACAAAGCTGCCCTGACCCTTTCGGGTG17250.16936904385529108No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCC17020.16711078993722053No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16980.16671805012538218No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16910.16603075545466506No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16770.1646561661132308No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16690.1638706864895541No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16400.16102332285372603No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16320.1602378432300493No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15910.15621226015870615No Hit
GCCTGAAGATGAGGCTGACTATTTTTGCTTGCTCTCCTATAGAGGTGCTGTGGTTTTCGGCGGAGGGACCAAG15590.15307034166399933No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15530.15248123194624177No Hit
AAGCTGCCCTGACCCTTTCGGGTGCGCTGCCTGAAGATGAGGCTGACTATTTTTGCTTGCTCTCCTATAGAGGTG15290.15012479307521165No Hit
GATTTATGATGCAAGCACCAGACACTCCTGGACGCCTGCCCGGTTCTCAGGCTACCTCCTTGGGGACAAAGCTGC15150.1487502037337774No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14240.1398153730144548No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14210.13952081815557602No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14120.13863715357893971No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14020.1376553040493438No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACTTGTGGCTCCA13970.1371643792845459No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13720.13470975546055616No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13580.1333351661191219No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13420.13176420687176849No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC13000.12764043884746576No Hit
TCTTCAGGCAGCGCACCCGAAAGGGTCAGGGCAGCTTTGTCCCCAAGGAGGTAGCCTGAGAACCGGGCAGGCGTC12730.12498944511755683No Hit
GGGCAGCTTTGTCCCCAAGGAGGTAGCCTGAGAACCGGGCAGGCGTCCAG12660.12430215044683972No Hit
GCACTGGAGCTGTCACCGATGGTCATTATCCCTACTGGTTCCAGCAGAAG12500.12273119119948629No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12410.12184752662284999No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12310.12086567709325412No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGC11900.11684009402191096No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11810.11595642944527465No Hit
GTCATTATCCCTACTGGTTCCAGCAGAAGCCTGGCCAAGCCCCCAGGACA11610.11399273038608286No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACTTGTGGCTCCAGC11610.11399273038608286No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11510.11301088085648699No Hit
GGCTACCTCCTTGGGGACAAAGCTGCCCTGACCCTTTCGGGTGCGCTGCCTGAAGATGAGGCTGACTATTTTTG11400.1119308463739315No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11340.11134173665617397No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11210.11006533226769931No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11070.10869074292626506No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10980.10780707834962876No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10680.1048615297608411No Hit
GAGGTAGCCTGAGAACCGGGCAGGCGTCCAGGAGTGTCTGGTGCTTGCAT10500.10309420060756848No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10320.10132687145429588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT852.8932845E-853.96883870
GTACGTA855.456968E-1251.16265569
CTAACGC200.00786843850.880813
GGTATCA13700.047.0754551
TAACGAT1751.5825208E-1037.44776570
GTATCAA35350.035.4322241
ACAACGC400.002822367733.917214
TATCAAC39200.031.7650432
TCAACGC39800.031.1901974
ATCAACG39700.031.186393
ACGCTTT450.00428155831.15052649
CAACGCA40550.030.9463675
AACGCAG40300.030.8858686
TTCGATC450.00466436730.61007539
GAATACG2700.030.145679
CGCAGAG41400.029.9833098
ACGCAGA42150.029.6107257
CAGAGTA42650.029.18263610
AGAGTAC42450.029.16034311
GGTACGT1202.6968337E-829.12972568
Produced by FastQC (version 0.11.5)