FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935934
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41030.4383856126607218No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38190.4080415926764067No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA34750.37128686424470103No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26970.28816134471020394No Hit
GTATTACTGTGCGAGAAGCGAGGCCGAAGGGGGGTTCCTGGTGCCCCGAC25260.26989082563514094No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATT24670.2635869623285403No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24320.2598473824008958No Hit
GAGTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATTTTGCAGTTTA23380.24980393916665067No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21980.23484561945607274No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20950.22384056995471904No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20800.22223789284287138No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGT20730.2214899768573425No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGACAATAAGGTCGCCTGGTATCAACAGAAACCTGGCCAGTCTCCCAGGCT20500.21903253861917613No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA20390.21785724207048787No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20390.21785724207048787No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20280.21668194552179962No Hit
ATCATAACTGGGCTTGCGCTTTCGGCCCTGGGACCAAAGTGGATGTCAGA20240.21625456495864026No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19820.2117670690454669No Hit
CAGTAATACAGGGCCGTGTCCGCGACAGTCACAGAGTTCAGGGTCAGGGAGATCTGGTTCTTAGACGTGTCGA19750.21101915305993799No Hit
GTAATACAGGGCCGTGTCCGCGACAGTCACAGAGTTCAGGGTCAGGGAGA19720.21069861763756847No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19120.20428790919017792No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19010.20311261264148966No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18870.2016167806704319No Hit
GTTTATTACTGTCAACACTATCATAACTGGGCTTGCGCTTTCGGCCCTGGGACCAAAGTGGATGTCAGACGAA18550.19819773616515696No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17950.19178702771776643No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC17690.18900905405723054No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17660.188688518634861No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA17150.18323941645457906No Hit
GTTATGATAGTGTTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAACT16990.1815298942019416No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16240.17351650864270346No Hit
CAATAAGGTCGCCTGGTATCAACAGAAACCTGGCCAGTCTCCCAGGCTCATCATCTATGGTGCATCCACCAGGGC16180.17287543779796438No Hit
GTCTCCAGTCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT16020.1711659155453269No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCA15770.16849478702558085No Hit
CTATCATAACTGGGCTTGCGCTTTCGGCCCTGGGACCAAAGTGGATGTCAGACGAACTGTGGCTGCACCAT15220.16261830428213955No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA14590.1558870604123795No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14570.15567337013079982No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14550.15545967984922013No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14330.15310908675184362No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT14240.15214748048473503No Hit
GTGCAGCCACAGTTCGTCTGACATCCACTTTGGTCCCAGGGCCGAAAGCG14070.15033111309130773No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGTCACCCTGTCTGTGTCTCCAGG14040.1500105776689382No Hit
CTCCCAGGCTCATCATCTATGGTGCATCCACCAGGGCCACTGGTGTCCCA13740.14680522344524294No Hit
CCATAGATGATGAGCCTGGGAGACTGGCCAGGTTTCTGTTGATACCAGGC13680.1461641526005039No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13390.14306564351759846No Hit
CCAGTGGCCCTGGTGGATGCACCATAGATGATGAGCCTGGGAGACTGGCCAGGTTTCTGTTGATACCAGGCGAC13380.14295879837680864No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13280.1418903469689102No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13240.14146296640575085No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13200.14103558584259146No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12990.1387918378860048No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12780.1365480899294181No Hit
GTCTCTGGTGGCTCCATCAACAGCGGCGATTTCTACTGGGTTTGGATCCG12750.13622755450704857No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGACAATAAGGTCGC12580.13441118711362127No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12440.13291535514256347No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12360.13206059401624473No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12210.1304579169043971No Hit
CTGTATTACTGTGCGAGAAGCGAGGCCGAAGGGGGGTTCCTGGTGCCCCG12070.1289620849333393No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11880.1269320272583323No Hit
GGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGT11870.12682518211754248No Hit
CTGCTACTCTGGCTCCCAGATATCACTGGAGAAATAGTGATGACGCAGTC11460.12244453134515895No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCTTCCTGCTGGTGGCAGCTCCCAGATGGGTC11420.12201715078199958No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11300.1207350090925215No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11240.12009393824778242No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11170.11934602226225353No Hit
CTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT10900.11646120346092781No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10890.11635435832013795No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10850.1159269777569786No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10850.1159269777569786No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG10800.11539275205302939No Hit
GTCAGAGTGTTGACAATAAGGTCGCCTGGTATCAACAGAAACCTGGCCAGTCTCCCAGGCTCATCATCTATGGTG10790.11528590691223953No Hit
ACAGAAACCTGGCCAGTCTCCCAGGCTCATCATCTATGGTGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTT10610.11336269437802239No Hit
CACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGA10550.11272162353328331No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGACAATAAGGTCGCCTGGTATCAACAGAAACCTGGCCAGT10530.11250793325170365No Hit
GAGTGTTGACAATAAGGTCGCCTGGTATCAACAGAAACCTGGCCAGTCTCCCAGGCTCATCATCTATGGTGCATC10510.11229424297012397No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10490.11208055268854428No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10240.10940942416879822No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10240.10940942416879822No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10230.10930257902800838No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10020.10705883107142171No Hit
ACCTTATTGTCAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACACAGACAGGGTGA10010.10695198593063188No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9810.10481508311483503No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCACTGGAGAAATAGTGATGACGCAGTCTC9800.10470823797404517No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9740.10406716712930612No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCACTGGAGAAATAGTGATGACGCAGTCTCCAGTCACC9720.10385347684772644No Hit
TCTATGGTGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT9690.10353294142535692No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9520.10171657403192962No Hit
GCCACAGTTCGTCTGACATCCACTTTGGTCCCAGGGCCGAAAGCGCAAGC9410.10054127748324133No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)