FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935934
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40770.43560763900018595No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA32000.34190445052749446No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30410.32491607314190957No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27210.29072562808916014No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATT25480.27224141873251745No Hit
GTATTACTGTGCGAGAAGCGAGGCCGAAGGGGGGTTCCTGGTGCCCCGAC23690.25311613853113574No Hit
GAGTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATTTTGCAGTTTA23410.25012447458902015No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23340.24937655860349126No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22810.24371376614162965No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22100.23612776114555087No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21340.22800753044552288No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20860.22287896368761045No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGACAATAAGGTCGCCTGGTATCAACAGAAACCTGGCCAGTCTCCCAGGCT19460.20792064397703258No Hit
ATCATAACTGGGCTTGCGCTTTCGGCCCTGGGACCAAAGTGGATGTCAGA19070.20375368348622874No Hit
GTAATACAGGGCCGTGTCCGCGACAGTCACAGAGTTCAGGGTCAGGGAGA18920.20215100637438108No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGT18920.20215100637438108No Hit
GTTTATTACTGTCAACACTATCATAACTGGGCTTGCGCTTTCGGCCCTGGGACCAAAGTGGATGTCAGACGAA18900.2019373160928014No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18780.2006551744033233No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA18750.2003346389809538No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18560.1983045813059468No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18400.19659505905330932No Hit
GTTATGATAGTGTTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAACT17900.1912528020138172No Hit
CAGTAATACAGGGCCGTGTCCGCGACAGTCACAGAGTTCAGGGTCAGGGAGATCTGGTTCTTAGACGTGTCGA17830.19050488602828833No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA17120.18291888103220952No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17020.1818504296243111No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17000.18163673934273145No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16900.180568287934833No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16830.17982037194930411No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16610.17746977885192758No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC16530.17661501772560884No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16060.17159329610848628No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCA15710.16785371618084183No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15640.1671058001953129No Hit
GTCTCCAGTCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT15000.16026771118476305No Hit
CAATAAGGTCGCCTGGTATCAACAGAAACCTGGCCAGTCTCCCAGGCTCATCATCTATGGTGCATCCACCAGGGC14890.15909241463607476No Hit
GTGCAGCCACAGTTCGTCTGACATCCACTTTGGTCCCAGGGCCGAAAGCG14760.15770342780580682No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14370.15353646731500298No Hit
CTATCATAACTGGGCTTGCGCTTTCGGCCCTGGGACCAAAGTGGATGTCAGACGAACTGTGGCTGCACCAT14340.15321593189263344No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT14150.15118587421762644No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14120.15086533879525693No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14070.15033111309130773No Hit
GTCTCTGGTGGCTCCATCAACAGCGGCGATTTCTACTGGGTTTGGATCCG13390.14306564351759846No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGTCACCCTGTCTGTGTCTCCAGG13150.14050136013864226No Hit
CTCCCAGGCTCATCATCTATGGTGCATCCACCAGGGCCACTGGTGTCCCA13140.1403945149978524No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12870.13750969619652667No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12610.13473172253599078No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12490.13344958084651268No Hit
CTGCTACTCTGGCTCCCAGATATCACTGGAGAAATAGTGATGACGCAGTC12410.13259481972019393No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA12400.13248797457940412No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12370.13216743915703458No Hit
CCATAGATGATGAGCCTGGGAGACTGGCCAGGTTTCTGTTGATACCAGGC12180.13013738148202758No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12170.13003053634123773No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGACAATAAGGTCGC12040.1286415495109698No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11960.12778678838465105No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11830.1263978015543831No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11760.1256498855688542No Hit
CCAGTGGCCCTGGTGGATGCACCATAGATGATGAGCCTGGGAGACTGGCCAGGTTTCTGTTGATACCAGGCGAC11610.12404720845700659No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11480.12265822162673863No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCTTCCTGCTGGTGGCAGCTCCCAGATGGGTC11470.1225513764859488No Hit
ACAGAAACCTGGCCAGTCTCCCAGGCTCATCATCTATGGTGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTT11440.12223084106357926No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11400.1218034605004199No Hit
CTGTATTACTGTGCGAGAAGCGAGGCCGAAGGGGGGTTCCTGGTGCCCCG11350.1212692347964707No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11320.12094869937410116No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11290.12062816395173163No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11260.12030762852936211No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11030.11785019029119574No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10730.11464483606750048No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10670.11400376522276144No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10610.11336269437802239No Hit
GGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGT10420.11133263670301538No Hit
CTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT10320.11026418529511697No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGACATCCACTTTGGTCCCAGGGCCGAAAGCGCAAGCCCAGTT10280.10983680473195759No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10150.10844781790168964No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10090.10780674705695059No Hit
GAGTGTTGACAATAAGGTCGCCTGGTATCAACAGAAACCTGGCCAGTCTCCCAGGCTCATCATCTATGGTGCATC9940.10620406994510297No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGACAATAAGGTCGCCTGGTATCAACAGAAACCTGGCCAGT9670.10331925114377724No Hit
GTCAGAGTGTTGACAATAAGGTCGCCTGGTATCAACAGAAACCTGGCCAGTCTCCCAGGCTCATCATCTATGGTG9570.10225079973587882No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCACTGGAGAAATAGTGATGACGCAGTCTCCAGTCACC9500.10150288375034991No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9500.10150288375034991No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9460.10107550318719054No Hit
GCCTGGGAGACTGGCCAGGTTTCTGTTGATACCAGGCGACCTTATTGTCA9450.1009686580464007No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9360.10000705177929212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG309.919375E-656.6952972
CTAACGC252.7534488E-454.4216773
TAAACGA200.00778518251.0175934
GGGCAAA8200.041.64204870
CGGAAAT501.9690851E-434.1705630
GGGGCAA7350.034.11095469
GTATAGA400.002780367934.0208131
GACTGAT6650.033.22523570
ATCAACG21550.031.4092623
TCAACGC21800.031.0474014
CAACGCA22150.030.8639145
GTGGTAT2700.030.2407261
CTAGATG450.004954698530.2342643
TATACAG450.00495599330.2326475
AACGCAG22900.030.0016126
TTCGATC605.472992E-428.70349139
CGCAGAG23850.028.5213648
TTTCGGG605.865352E-428.36583719
AGTACAC605.891085E-428.344623
GACAGGT2050.028.2123831
Produced by FastQC (version 0.11.5)