FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482845
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTGTGATATTCGG32760.6784786007932152No Hit
GTAATAATCAGTCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG30540.632501113193675No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22150.45873934699541263No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT22090.45749671219542504No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACACCACTCAGGCTGTCATCCCATG18680.38687363439613126No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17730.367198583396328No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC16410.3398606177966014No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15780.32681295239673186No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC15250.3158363449968416No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13980.28953390839710463No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13240.2742080791972579No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13010.26944464579730554No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACACCACTCAGG12680.2626101543973739No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT12380.256396980397436No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11840.24521326719754788No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11800.24438484399755614No Hit
GTAATACACGGCCGTGTCTGAGGCGGTCACAGAGCTCAGCTTCAGGGAGA11480.2377574583976224No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11040.22864480319771358No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10160.21041949279789582No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9920.20544895359794554No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT9880.20462053039795378No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9790.20275657819797244No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9740.20172104919798278No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9700.20089262599799107No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9570.19820025059801802No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG9560.19799314479802005No Hit
GTGTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC9500.19675050999803248No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9230.1911586533980884No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG8940.18515258519814848No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC8850.1832886329981671No Hit
ATATCACACCACTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGTC8740.1810104691981899No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC8710.18038915179819612No Hit
CCCCTGAGGGCCGCTGATTATTCCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACCTGCTGGTACCAGGATA8560.17728256479822718No Hit
GTCTCTGGTGGCTCCATCATCAGTGAAACTTACTACTGGAGCTGGATCCG8320.17231202559827688No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG8260.1710693907982893No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8110.16796280379832038No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8100.16775569799832246No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7880.16319937039836802No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA7810.1617496297983825No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7530.1559506673984405No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC7320.15160144559848399No Hit
GATTATTCCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACCTGCTGG7240.14994459919850056No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT7090.14683801219853163No Hit
GCATGGGATGACAGCCTGAGTGGTGTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG6920.1433172135985668No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6890.14269589619857304No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC6760.14000352079859998No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACACCACTCAGGC6690.13855378019861447No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC6590.13648272219863516No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6540.13544719319864554No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACAC6530.1352400873986476No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6470.13399745259866003No Hit
GTGTATTACTGTGCGAGAGCCCCCCTATACCAATTCCCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGT6300.13047665399869524No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT6230.12902691339870972No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6230.12902691339870972No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT6190.128198490198718No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA6110.1265416437987346No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC6110.1265416437987346No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT6020.12467769159875322No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5960.12343505679876564No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC5940.1230208451987698No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG5800.12012136399879877No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG5780.11970715239880292No Hit
CAGTAATACACGGCCGTGTCTGAGGCGGTCACAGAGCTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTA5750.11908583499880913No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTATGTATCCTGGTACCAGCAGGTCCCAGGAACGGCCCC5750.11908583499880913No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5600.11597924799884021No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGACTGATTATTACTGTGCAG5480.11349397839886506No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC5420.11225134359887749No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG5400.11183713199888162No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC5390.11163002619888371No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5340.11059449719889405No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCATCAGTGAAACTTACTACTGGA5330.11038739139889614No Hit
GGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG5230.10831633339891684No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5150.10665948699893339No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC5140.10645238119893548No Hit
GTATAGGGGGGCTCTCGCACAGTAATACACGGCCGTGTCTGAGGCGGTCA5120.10603816959893961No Hit
ATCCCATGCTGCACAGTAATAATCAGTCTCATCCTCGGACCGGAGCCCAC5080.10520974639894791No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT4960.10272447679897277No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC4940.10231026519897689No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG4920.10189605359898105No Hit
GGATGAGACTGATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTGTGATATTCGGCGGAGGGACCAAG4890.10127473619898726No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG4880.10106763039898932No Hit
GTCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCC4860.10065341879899348No Hit
GTGCAGCATGGGATGACAGCCTGAGTGGTGTGATATTCGGCGGAGGGACC4830.10003210139899968No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGG150.002467509168.190571
CTAACGC200.00772042151.121743
GCCCGGT200.007723583451.11644418
GGTATCA6300.043.8367961
GCCAGAG9850.043.73881570
AAAGGGG2750.043.66549368
AAAAGGG2750.041.36218367
CGTATGC3850.041.3282441
TCGTATG3850.041.2718340
CCGTCTT3850.040.70366747
TCTCGTA3900.040.64030538
CTACATC3800.040.6286133
CTTATAC3950.040.5690731
ATCTCCG3950.040.54806510
CCCACGA3900.040.19412219
ATGCCGT3900.040.07155244
CGTCTTC3950.039.7320748
ACGAGAC4050.039.55097622
CACGAGA4050.039.5468821
TCTCCGA4050.039.5468811
Produced by FastQC (version 0.11.5)