FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264770
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTCTTTTGACTCAGCCCTCCTCAGCGTCTGGGACCC73200.5787613558196352No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC65480.5177225898780016No Hit
GTTCTAATACTGTTAGCTGGTATCAGCAGGTCCCAGGAACGGCCCCCAGA56160.44403330249768735No Hit
GCCTTGGGCTGACCCAGGACGGTCAACCTGGTCCCTCCGCCGAAAACCGGACCATGCAGGGAGTCATCCCACG55690.4403172118250749No Hit
ATTTATTCCTGTGCAGCGTGGGATGACTCCCTGCATGGTCCGGTTTTCGG55340.43754991026036355No Hit
GGAATAAATAGCCTCATCCTCAGACCGGAGGCCATTGATGGCCAGGGAGG53280.4212623639080623No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG42810.33848051424369646No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35510.2807625101797165No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC34730.2745953809783597No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA34120.26977236967986273No Hit
GTATTAGAACCGATGTTGGAGCTGCTTCCAGAACAAGAAATGGTGACACT33020.2610751361907699No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT32900.2601263470828688No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACCTGGTCCCTC32430.2564102564102564No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG30730.24296907738165832No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT30440.24067617037089747No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC30430.24059710461190573No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30280.23941111822702943No Hit
CTCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCAATG30210.23885765791408714No Hit
CTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCAATGTG29170.23063481897894478No Hit
CTCCCTGCATGGTCCGGTTTTCGGCGGAGGGACCAGGTTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGT29130.2303185559429778No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27930.22083066486396738No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG26820.21205436561588273No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26550.2099195901231054No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCAGGGCTC25530.20185488270594654No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25070.1982178577923259No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG25050.19805972627434235No Hit
GCCCTCCTCAGCGTCTGGGACCCCCGGGCAGAGTGTCACCATTTCTTGTTCTGGAAGCAGCTCCAACATCGGTT24780.19592495078156502No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24240.19165539979601035No Hit
CCTCAGGGGTCACTGGCCGATTCTCTGGGTCCAAGTCTGGCACCTCGGCCTCCCTGGCCATCAATGGCCTCCGGT23960.18944155854424125No Hit
ATGCAGGGAGTCATCCCACGCTGCACAGGAATAAATAGCCTCATCCTCAG23760.18786024336440615No Hit
CCCCTGAGGGCCGCTGATCATTGATATAGATGAGGAGTCTGGGGGCCGTTCCTGGGACCTGCTGATACCAGCTAA23060.18232564023498343No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC22820.18042806201918135No Hit
CTGTCAGGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC22440.17742356317749472No Hit
GTGCAGGGTCCTGGGCCCAGTCTCTTTTGACTCAGCCCTCCTCAGCGTCT21750.17196802580706372No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACACTCTCCAGCATGGCCAG21690.1714936312531132No Hit
CTCCTCATCTATATCAATGATCAGCGGCCCTCAGGGGTCACTGGCCGATT20930.16548463356973994No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCCTGACAGTGGC20890.16516837053377292No Hit
GGATGACTCCCTGCATGGTCCGGTTTTCGGCGGAGGGACCAGGTTGACCGTCCTGGGTCAGCCCAAGGCTGCCCC20750.1640614499078884No Hit
ACTCAGGACACTCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCAATGTGCAGGGTCCTGGGC20460.16176854289712753No Hit
ACCCAGGACGGTCAACCTGGTCCCTCCGCCGAAAACCGGACCATGCAGGG20380.16113601682519352No Hit
GTCTGGCACCTCGGCCTCCCTGGCCATCAATGGCCTCCGGTCTGAGGATG20280.16034535923527599No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC20190.1596337674043502No Hit
GGGTAGAGAAGACAGGACTCAGGACACTCTCCAGCATGGCCAGCTTCCCT20150.15931750436838318No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20110.1590012413324162No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19820.15670833432165532No Hit
GCCCCCAGACTCCTCATCTATATCAATGATCAGCGGCCCTCAGGGGTCACTGGCCGATTCTCTGGGTCCAAGT19490.15409916427492745No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18710.1479320350735707No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACCTGGTCCCTCCGCCGAAAAC18430.1457181938218016No Hit
GCTATCTGCCTTCCAGGCCACTGTCAGGGCTCCCGGGTAGAAGTCACTTA18350.14508566774986756No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18130.14334622105204897No Hit
AGACAGGACTCAGGACACTCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCAATGTGCAGGGT18130.14334622105204897No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17930.1417649058722139No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACACTCTCCAGCATGGCCAGCTTCCCTCTC17870.1412905113182634No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT17800.14073705100532113No Hit
CTTTTGACTCAGCCCTCCTCAGCGTCTGGGACCCCCGGGCAGAGTGTCAC17790.1406579852463294No Hit
GTCTGGGGGCCGTTCCTGGGACCTGCTGATACCAGCTAACAGTATTAGAA17760.14042078796935412No Hit
CATCTATATCAATGATCAGCGGCCCTCAGGGGTCACTGGCCGATTCTCTGGGTCCAAGTCTGGCACCTCGGCCTC17610.1392348015844778No Hit
GCGTGGGATGACTCCCTGCATGGTCCGGTTTTCGGCGGAGGGACCAGGTTGACCGTCCTGGGTCAGCCCAAGG17580.13899760430750255No Hit
GGTATCAGCAGGTCCCAGGAACGGCCCCCAGACTCCTCATCTATATCAAT17530.13860227551254378No Hit
CCTCAGCGTCTGGGACCCCCGGGCAGAGTGTCACCATTTCTTGTTCTGGAAGCAGCTCCAACATCGGTTCTAATA17290.1367046972967417No Hit
GACCCAGGACGGTCAACCTGGTCCCTCCGCCGAAAACCGGACCATGCAGG17240.13630936850178293No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC17090.13512338211690664No Hit
GTCTGAGGATGAGGCTATTTATTCCTGTGCAGCGTGGGATGACTCCCTGCATGGTCCGGTTTTCGGCGGAGGGAC17080.13504431635791486No Hit
TCCTCAGACCGGAGGCCATTGATGGCCAGGGAGGCCGAGGTGCCAGACTTGGACCCAGAGAATCGGCCAGTGACC16760.13251421207017877No Hit
GTCCAAGTCTGGCACCTCGGCCTCCCTGGCCATCAATGGCCTCCGGTCTG16400.12966784474647564No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16190.12800746380764882No Hit
GAGTGTCACCATTTCTTGTTCTGGAAGCAGCTCCAACATCGGTTCTAATA16170.1278493322896653No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT16160.12777026653067355No Hit
GTGCCAGACTTGGACCCAGAGAATCGGCCAGTGACCCCTGAGGGCCGCTG15380.12160313732931678No Hit
GTGTCACCATTTCTTGTTCTGGAAGCAGCTCCAACATCGGTTCTAATACT15320.12112874277536627No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACACTCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA15030.11883583576460544No Hit
GCACAGGAATAAATAGCCTCATCCTCAGACCGGAGGCCATTGATGGCCAGGGAGGCCGAGGTGCCAGACTTGGAC15020.11875677000561366No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCCTGACAGTGGCCTGGA14630.11567320540493528No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCAATGTGCAGGGTCCTGGGCCCAGTCTCTTTTGACTCAGCCCTCCTCAG14370.11361749567114969No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14340.11338029839417445No Hit
TCCTCACCCTCCTCACTCAATGTGCAGGGTCCTGGGCCCAGTCTCTTTTGACTCAGCCCTCCTCAGCGTCTGGGA14330.11330123263518267No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14230.11251057504526515No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACACTCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC14050.11108739138341359No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG14010.11077112834744657No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCAGGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13950.11029673379349604No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC13950.11029673379349604No Hit
GATGAGGAGTCTGGGGGCCGTTCCTGGGACCTGCTGATACCAGCTAACAG13910.10998047075752905No Hit
TTCCAGGCCACTGTCAGGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13890.10982233923954553No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGACGTCAACCTCAGGTAAAATTCATTCTTGGACCTGTCCACCGA13790.109031681649628No Hit
ACCTGGTCCCTCCGCCGAAAACCGGACCATGCAGGGAGTCATCCCACGCTGCACAGGAATAAATAGCCTCATCCT13780.10895261589063623No Hit
CCCTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTG13690.10824102405971045No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCCTGACAGTGGCCTGGAAGGCAGATAGCAGCC13540.10705503767483417No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACCTGGTC13460.10642251160290013No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC13290.10507839370004032No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13060.10325988124322999No Hit
GTGTATTACTGCGCGAGGAATCACGTCACGCGGATGAAAAATGGTTATAC13060.10325988124322999No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13050.10318081548423824No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACACTCTCCAGCATGGCCAGCTTCCCTCTCCTC13040.10310174972524648No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13020.10294361820726299No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12880.10183669758137844No Hit
GCTGATACCAGCTAACAGTATTAGAACCGATGTTGGAGCTGCTTCCAGAA12830.10144136878641966No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG12740.10072977695549387No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12740.10072977695549387No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG352.4979281E-548.5054322
TCGTATG3450.048.0145940
CGTATGC3500.047.41631741
AGGGGGG4350.046.6580670
AATCTCG3400.046.39608436
TCTCGTA3350.046.19586638
GCTAATC3300.045.5526933
ACGAGAC3750.044.37156322
CACGAGA3850.043.21734221
ATGCCGT3750.042.61025644
TAACGCC405.4961936E-542.4422534
CGAGCCC4000.042.44057515
CTTATAC3850.042.337031
GTGGTAT2900.042.1545451
CCCACGA4050.041.9182719
TTATACA3900.041.7892952
CGAGACT4000.041.60327523
ATCTCGT3800.041.56706237
GTATGCC4050.041.04150442
GCCGTCT3600.040.67082246
Produced by FastQC (version 0.11.5)