FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264770
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTCTTTTGACTCAGCCCTCCTCAGCGTCTGGGACCC66630.5268151521620532No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC64110.5068905808961313No Hit
GCCTTGGGCTGACCCAGGACGGTCAACCTGGTCCCTCCGCCGAAAACCGGACCATGCAGGGAGTCATCCCACG57290.4529677332637555No Hit
GTTCTAATACTGTTAGCTGGTATCAGCAGGTCCCAGGAACGGCCCCCAGA55010.43494074021363566No Hit
ATTTATTCCTGTGCAGCGTGGGATGACTCCCTGCATGGTCCGGTTTTCGG53570.42355527091882317No Hit
GGAATAAATAGCCTCATCCTCAGACCGGAGGCCATTGATGGCCAGGGAGG47670.3769064731136887No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44760.3538983372470884No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43780.34614989286589654No Hit
GTATTAGAACCGATGTTGGAGCTGCTTCCAGAACAAGAAATGGTGACACT34850.27554417008626075No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC34800.27514884129130196No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG34700.27435818370138443No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33520.26502842414035754No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31340.2477920886801553No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG31000.2451038528744357No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACCTGGTCCCTC30430.24059710461190573No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT29780.23545783027744174No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC29780.23545783027744174No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28910.2285791092451592No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28870.22826284620919216No Hit
CTCCCTGCATGGTCCGGTTTTCGGCGGAGGGACCAGGTTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGT28490.22525834736750555No Hit
ATGCAGGGAGTCATCCCACGCTGCACAGGAATAAATAGCCTCATCCTCAG28120.22233291428481067No Hit
CTCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCAATG27010.21355661503672604No Hit
CTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCAATGTG26180.20699415704041052No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG25770.20375246092174862No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25510.20169675118796304No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCAGGGCTC25440.20114329087502075No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG24720.1954505562276145No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24330.19236699162693613No Hit
CCCCTGAGGGCCGCTGATCATTGATATAGATGAGGAGTCTGGGGGCCGTTCCTGGGACCTGCTGATACCAGCTAA22930.18129778536809066No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACCTGGTCCCTCCGCCGAAAAC22750.17987460170623906No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22230.1757631822386679No Hit
GCCCTCCTCAGCGTCTGGGACCCCCGGGCAGAGTGTCACCATTTCTTGTTCTGGAAGCAGCTCCAACATCGGTT21900.17315401219194004No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACACTCTCCAGCATGGCCAG21830.17260055187899775No Hit
CCTCAGGGGTCACTGGCCGATTCTCTGGGTCCAAGTCTGGCACCTCGGCCTCCCTGGCCATCAATGGCCTCCGGT21350.1688053954473936No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC20830.1646939759798224No Hit
CTCCTCATCTATATCAATGATCAGCGGCCCTCAGGGGTCACTGGCCGATT20780.16429864718486364No Hit
GTGCAGGGTCCTGGGCCCAGTCTCTTTTGACTCAGCCCTCCTCAGCGTCT20770.1642195814258719No Hit
GGATGACTCCCTGCATGGTCCGGTTTTCGGCGGAGGGACCAGGTTGACCGTCCTGGGTCAGCCCAAGGCTGCCCC20760.16414051566688015No Hit
CTGTCAGGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC20280.16034535923527599No Hit
ACCCAGGACGGTCAACCTGGTCCCTCCGCCGAAAACCGGACCATGCAGGG20120.15908030709140794No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19800.15655020280367182No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19680.15560141369577077No Hit
ACTCAGGACACTCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCAATGTGCAGGGTCCTGGGC19350.1529922436490429No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19280.15243878333610064No Hit
GTCTGGCACCTCGGCCTCCCTGGCCATCAATGGCCTCCGGTCTGAGGATG19040.15054120512029853No Hit
GGGTAGAGAAGACAGGACTCAGGACACTCTCCAGCATGGCCAGCTTCCCT18920.1495924160123975No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18590.14698324596566964No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18540.14658791717071087No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCCTGACAGTGGC18520.14642978565272738No Hit
GTCTGGGGGCCGTTCCTGGGACCTGCTGATACCAGCTAACAGTATTAGAA18420.14563912806280985No Hit
GCTATCTGCCTTCCAGGCCACTGTCAGGGCTCCCGGGTAGAAGTCACTTA17720.1401045249333871No Hit
GTCTGAGGATGAGGCTATTTATTCCTGTGCAGCGTGGGATGACTCCCTGCATGGTCCGGTTTTCGGCGGAGGGAC17700.1399463934154036No Hit
GCGTGGGATGACTCCCTGCATGGTCCGGTTTTCGGCGGAGGGACCAGGTTGACCGTCCTGGGTCAGCCCAAGG17430.13781161792262625No Hit
GCCCCCAGACTCCTCATCTATATCAATGATCAGCGGCCCTCAGGGGTCACTGGCCGATTCTCTGGGTCCAAGT17400.13757442064565098No Hit
GAGTCATCCCACGCTGCACAGGAATAAATAGCCTCATCCTCAGACCGGAG17390.13749535488665923No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCCTGACAGTGGCCTGGA16940.13393739573203034No Hit
CATCTATATCAATGATCAGCGGCCCTCAGGGGTCACTGGCCGATTCTCTGGGTCCAAGTCTGGCACCTCGGCCTC16520.1306166338543767No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACACTCTCCAGCATGGCCAGCTTCCCTCTC16400.12966784474647564No Hit
CTTTTGACTCAGCCCTCCTCAGCGTCTGGGACCCCCGGGCAGAGTGTCAC16400.12966784474647564No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCAGGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16390.1295887789874839No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16370.1294306474695004No Hit
AGACAGGACTCAGGACACTCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCAATGTGCAGGGT16240.1284027926026076No Hit
CCTCAGCGTCTGGGACCCCCGGGCAGAGTGTCACCATTTCTTGTTCTGGAAGCAGCTCCAACATCGGTTCTAATA16150.1276912007716818No Hit
GGTATCAGCAGGTCCCAGGAACGGCCCCCAGACTCCTCATCTATATCAAT15980.126347082868822No Hit
GTGCCAGACTTGGACCCAGAGAATCGGCCAGTGACCCCTGAGGGCCGCTG15880.12555642527890448No Hit
GAGTGTCACCATTTCTTGTTCTGGAAGCAGCTCCAACATCGGTTCTAATA15760.12460763617100341No Hit
GTGTCACCATTTCTTGTTCTGGAAGCAGCTCCAACATCGGTTCTAATACT15630.12357978130411063No Hit
GACCCAGGACGGTCAACCTGGTCCCTCCGCCGAAAACCGGACCATGCAGG15610.12342164978612713No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15480.12239379491923433No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC15360.12144500581133329No Hit
ACCTGGTCCCTCCGCCGAAAACCGGACCATGCAGGGAGTCATCCCACGCTGCACAGGAATAAATAGCCTCATCCT15230.12041715094444048No Hit
GTCCAAGTCTGGCACCTCGGCCTCCCTGGCCATCAATGGCCTCCGGTCTG15080.1192311645595642No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACACTCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA15030.11883583576460544No Hit
TCCTCAGACCGGAGGCCATTGATGGCCAGGGAGGCCGAGGTGCCAGACTTGGACCCAGAGAATCGGCCAGTGACC14870.11757078362073738No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14580.11527787660997652No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14360.11353842991215794No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGACGTCAACCTCAGGTAAAATTCATTCTTGGACCTGTCCACCGA14300.11306403535820743No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACCTGGTC14190.11219431200929814No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGT14130.11171991745534762No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG13820.10926887892660327No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACACTCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC13780.10895261589063623No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13730.10855728709567747No Hit
GCACAGGAATAAATAGCCTCATCCTCAGACCGGAGGCCATTGATGGCCAGGGAGGCCGAGGTGCCAGACTTGGAC13540.10705503767483417No Hit
GATGAGGAGTCTGGGGGCCGTTCCTGGGACCTGCTGATACCAGCTAACAG13240.10468306490508156No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC13190.10428773611012279No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13180.10420867035113104No Hit
TCCTCACCCTCCTCACTCAATGTGCAGGGTCCTGGGCCCAGTCTCTTTTGACTCAGCCCTCCTCAGCGTCTGGGA13080.1034180127612135No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13060.10325988124322999No Hit
TTCCAGGCCACTGTCAGGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12760.1008879084734774No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCAATGTGCAGGGTCCTGGGCCCAGTCTCTTTTGACTCAGCCCTCCTCAG12700.10041351391952688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGTA200.0064744753.45346557
CCTAACG200.00787763550.8664932
TATACGT200.00788256250.8584485
GTGGTAT3450.039.3310931
GTATCAA32700.037.6577151
TATCAAC37050.033.133012
ATCAACG38700.031.5426183
TCAACGC38950.031.511784
CCACCGA5700.031.22759269
CAACGCA39550.031.0337285
AACGCAG39600.030.9089246
GCCGTTA803.1566342E-629.67446715
CGCAGAG42350.028.8217898
GTGCTAG2600.028.7041321
ACGCAGA42700.028.6649517
CCCCTAT605.9847534E-428.2692241
AGAGTAC43250.028.06524311
AATGGTA5300.027.93273770
CCGTTAA855.0556955E-627.93222616
CAGAGTA43850.027.75854710
Produced by FastQC (version 0.11.5)