FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786841
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39720.5048033846736507No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34940.4440541354606585No Hit
GTTAGGACCCAGAGGGAACCATGGAAAGCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26810.3407295756067617No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25620.3256058085432762No Hit
CATCTATGCTGCAGCCACCCGGGCCACTGGCATCACAGACAAATTCAGTG24650.3132780320293427No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23430.29777299352728187No Hit
CTCCTGCAGGGCCAGTCAGATTATAAAAAGCGACCTGTTAAGTTGGTACC23230.2952311839367801No Hit
GTCTGGAACAGAGTTCATGCTCACCATCAACAGACTGGAGCCTGAAGATTTTGCTGTCTACTTCTGTCAGCAGT22990.292181012428178No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC22440.28519103605429813No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21100.2681609117979363No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20700.2630772926169328No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20470.26015421158785573No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18750.23829464910954057No Hit
CCATCAACAGACTGGAGCCTGAAGATTTTGCTGTCTACTTCTGTCAGCAG17790.2260939630751321No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17300.21986652957840278No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17280.21961234861935258No Hit
GTCTCCAGGCACCCTGTCTGTGTCTCCAGGGGAGACAGCCACCCTCTCCTGCAGGGCCAGTCAGATTATAAAAAG17250.21923107718077728No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16940.21529127231549958No Hit
GAGTTCATGCTCACCATCAACAGACTGGAGCCTGAAGATTTTGCTGTCTACTTCTGTCAGCAGTCTGGTGACTCA16810.21363909608167342No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16470.20931801977782044No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15140.19241498600098367No Hit
GTTAAGTTGGTACCAACAGAAACCTGGCCAGGCTCCCCGGCTCCTCATCTATGCTGCAGCCACCCGGGCCACTGG14910.18949190497190666No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14910.18949190497190666No Hit
GTCTGTGTCTCCAGGGGAGACAGCCACCCTCTCCTGCAGGGCCAGTCAGA14780.1878397287380805No Hit
GTCTACTTCTGTCAGCAGTCTGGTGACTCACCTTGGACGTTCGGCCAAGGGACCAGGGTGGACATCAAGGGAA14610.185679190586154No Hit
GTTCCAGACCCACTGCCACTGAATTTGTCTGTGATGCCAGTGGCCCGGGT14330.18212065715945153No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13650.17347850455174552No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13110.16661561865739077No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT13080.16623434721881547No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12870.16356544714878862No Hit
GGACCAGGGTGGACATCAAGGGAACTGTGGCTGCACCATCTGTCTTCATC12520.15911728036541056No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCCTTGATGTCCACCCTGGTCC12300.1563212898158586No Hit
GTGCAGCCACAGTTCCCTTGATGTCCACCCTGGTCCCTTGGCCGAACGTC12040.15301693734820632No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12040.15301693734820632No Hit
CCTTGGACGTTCGGCCAAGGGACCAGGGTGGACATCAAGGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG11890.15111058015533No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11770.14958549440102892No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11750.14933131344197875No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11690.14856877056482823No Hit
CCCTGGTCCCTTGGCCGAACGTCCAAGGTGAGTCACCAGACTGCTGACAGAAGTAGACAGCAAAATCTTCAGGCT11640.14793331816720276No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAAGCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11400.14488314665860066No Hit
GCATAGATGAGGAGCCGGGGAGCCTGGCCAGGTTTCTGTTGGTACCAACTTAACAGGTCGCTTTTTATAATCTGA11320.14386642282239995No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11200.14234133706809887No Hit
CCCTGTCTGTGTCTCCAGGGGAGACAGCCACCCTCTCCTGCAGGGCCAGT11120.14132461323189818No Hit
GAAGTAGACAGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGCATGAACTCTGTTCCAGACCCACTGCCA11080.14081625131379785No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11070.14068916083427274No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAAGCCCAGCGCAGCTTCTCT10880.13827444172329606No Hit
ACAGAAACCTGGCCAGGCTCCCCGGCTCCTCATCTATGCTGCAGCCACCCGGGCCACTGGCATCACAGACAAATT10820.13751189884614554No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10800.13725771788709537No Hit
CTCCAGGGGAGACAGCCACCCTCTCCTGCAGGGCCAGTCAGATTATAAAA10760.136749355968995No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10710.13611390357136957No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10660.13547845117374413No Hit
ATGCTGCAGCCACCCGGGCCACTGGCATCACAGACAAATTCAGTGGCAGTGGGTCTGGAACAGAGTTCATGCTCA10640.13522427021469396No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10560.13420754637849325No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10540.13395336541944305No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10510.1335720939808678No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGGGAGATTGTGTTGACGCAGTC10360.13166573678799148No Hit
GACAAATTCAGTGGCAGTGGGTCTGGAACAGAGTTCATGCTCACCATCAACAGACTGGAGCCTGAAGATTTTG10350.1315386463084664No Hit
GTGTTGACGCAGTCTCCAGGCACCCTGTCTGTGTCTCCAGGGGAGACAGC10270.13052192247226568No Hit
GTCTGGTGACTCACCTTGGACGTTCGGCCAAGGGACCAGGGTGGACATCAAGGGAACTGTGGCTGCACCAT10270.13052192247226568No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9840.12505703185268688No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9830.1249299413731618No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9520.12099013650788407No Hit
CTCCAGGCACCCTGTCTGTGTCTCCAGGGGAGACAGCCACCCTCTCCTGC9330.11857541739690737No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9220.1171774221221314No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9170.11654196972450596No Hit
GCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGGGAGATTGTGTTGACGCAGTCTC9140.11616069828593072No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9110.11577942684735543No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9070.11527106492925508No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9050.1150168839702049No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8930.11349179821590384No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8910.11323761725685368No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8650.10993326478920139No Hit
ATATTACTGTGCGCGAGGACATGAGGTTCGATCGTTTCAGCACTGGGGCC8580.10904363143252577No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8450.10739145519869961No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8440.10726436471917451No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8370.10637473136249892No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8360.10624764088297382No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8290.1053580075262982No Hit
CTCCAGGCACCCTGTCTGTGTCTCCAGGGGAGACAGCCACCCTCTCCTGCAGGGCCAGTCAGATTATAAAAAGCG8270.10510382656724801No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8230.10459546464914767No Hit
GTTCCCTTGATGTCCACCCTGGTCCCTTGGCCGAACGTCCAAGGTGAGTC8150.10357874081294698No Hit
CCCAGATACCACCGGGGAGATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTGTGTCTCCAGGGGAGACAGCCAC8060.10243492649722116No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT8060.10243492649722116No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8050.10230783601769607No Hit
CAAGGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT8030.10205365505864591No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7950.1010369312224452No Hit
CAGTAATATAGGGCCGTGTCCGCAGCGGTCACAGACCTCAATTCCAGGGAGAATTGGGTCTTGGACGTGTCAA7890.10027438834529466No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAA150.002214230670.0826448
ACGACTA150.002232733969.93574547
TATTACG200.00786306950.888527
TCGTATG2650.048.18863340
CGTATGC2750.047.78402741
CGCTAAT2650.047.6891732
ACACTCG2800.047.37706427
ACTCGCT2850.046.60527829
TATACAC2850.046.436423
ATCGCTA306.1273266E-446.27525743
TCGCTAA2900.045.87783431
GGTATCA8450.044.191691
GCTAATC2800.043.9426633
GTATGCC3200.043.30141442
ATCTCGT2800.042.94749537
AATCTCG2800.042.88401436
CACTCGC3100.042.8194728
ACGAGAC3100.042.70240422
GACGTAG405.500547E-542.4340671
ACGTAGA405.5129407E-542.417882
Produced by FastQC (version 0.11.5)