FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786841
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40360.5129371753632563No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30050.3819068909728903No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27500.3494988186939928No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24910.31658238449699494No Hit
GTCTGGAACAGAGTTCATGCTCACCATCAACAGACTGGAGCCTGAAGATTTTGCTGTCTACTTCTGTCAGCAGT24350.30946531764359No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23690.3010773459949342No Hit
GTTAGGACCCAGAGGGAACCATGGAAAGCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23600.2999335316792084No Hit
CATCTATGCTGCAGCCACCCGGGCCACTGGCATCACAGACAAATTCAGTG23080.29332482674390375No Hit
CTCCTGCAGGGCCAGTCAGATTATAAAAAGCGACCTGTTAAGTTGGTACC22180.2818866835866458No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21210.2695589070727123No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC20570.2614251163831066No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20000.25418095905017657No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17790.2260939630751321No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17480.22215415820985435No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16740.21274946272499784No Hit
GTCTCCAGGCACCCTGTCTGTGTCTCCAGGGGAGACAGCCACCCTCTCCTGCAGGGCCAGTCAGATTATAAAAAG16560.2104618340935462No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16520.20995347217544588No Hit
GAGTTCATGCTCACCATCAACAGACTGGAGCCTGAAGATTTTGCTGTCTACTTCTGTCAGCAGTCTGGTGACTCA16490.20957220073687058No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15730.19991332429296388No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15660.19902369093628824No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15540.1974986051819872No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15470.19660897182531162No Hit
CCATCAACAGACTGGAGCCTGAAGATTTTGCTGTCTACTTCTGTCAGCAG15120.1921608050419335No Hit
GTTAAGTTGGTACCAACAGAAACCTGGCCAGGCTCCCCGGCTCCTCATCTATGCTGCAGCCACCCGGGCCACTGG14380.18275610955707697No Hit
GTCTACTTCTGTCAGCAGTCTGGTGACTCACCTTGGACGTTCGGCCAAGGGACCAGGGTGGACATCAAGGGAA14310.18186647620040136No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14290.18161229524135117No Hit
GTTCCAGACCCACTGCCACTGAATTTGTCTGTGATGCCAGTGGCCCGGGT14010.17805376181464871No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13690.17398686646984587No Hit
GTCTGTGTCTCCAGGGGAGACAGCCACCCTCTCCTGCAGGGCCAGTCAGA13280.16877615680931726No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12130.15416075166393212No Hit
GTGCAGCCACAGTTCCCTTGATGTCCACCCTGGTCCCTTGGCCGAACGTC12030.1528898468686812No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11980.15225439447105577No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11780.14971258488055403No Hit
GGACCAGGGTGGACATCAAGGGAACTGTGGCTGCACCATCTGTCTTCATC11760.14945840392150384No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11690.14856877056482823No Hit
CCCTGGTCCCTTGGCCGAACGTCCAAGGTGAGTCACCAGACTGCTGACAGAAGTAGACAGCAAAATCTTCAGGCT11450.1455185990562261No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11420.14513732761765083No Hit
CTCCAGGCACCCTGTCTGTGTCTCCAGGGGAGACAGCCACCCTCTCCTGC11190.14221424658857382No Hit
GAAGTAGACAGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGCATGAACTCTGTTCCAGACCCACTGCCA11130.1414517037114233No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11080.14081625131379785No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCCTTGATGTCCACCCTGGTCC11070.14068916083427274No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10990.13967243699807202No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10980.13954534651854694No Hit
CCTTGGACGTTCGGCCAAGGGACCAGGGTGGACATCAAGGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG10830.13763898932567062No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAAGCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC10780.13700353692804518No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10650.13535136069421902No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10580.1344617273375434No Hit
GTCTGGTGACTCACCTTGGACGTTCGGCCAAGGGACCAGGGTGGACATCAAGGGAACTGTGGCTGCACCAT10570.13433463685801833No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAAGCCCAGCGCAGCTTCTCT10530.133826274939918No Hit
GTGTTGACGCAGTCTCCAGGCACCCTGTCTGTGTCTCCAGGGGAGACAGC10400.13217409870609184No Hit
GGGTGGACATCAAGGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG10120.12861556527938936No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9940.12632793664793776No Hit
CTCCAGGGGAGACAGCCACCCTCTCCTGCAGGGCCAGTCAGATTATAAAA9910.1259466652093625No Hit
ACAGAAACCTGGCCAGGCTCCCCGGCTCCTCATCTATGCTGCAGCCACCCGGGCCACTGGCATCACAGACAAATT9890.12569248425031232No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9690.12315067465981057No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9640.12251522226218513No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGGGAGATTGTGTTGACGCAGTC9620.12226104130313495No Hit
GCATAGATGAGGAGCCGGGGAGCCTGGCCAGGTTTCTGTTGGTACCAACTTAACAGGTCGCTTTTTATAATCTGA9580.12175267938503459No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9540.12124431746693423No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9530.12111722698740915No Hit
ATGCTGCAGCCACCCGGGCCACTGGCATCACAGACAAATTCAGTGGCAGTGGGTCTGGAACAGAGTTCATGCTCA9320.11844832691738229No Hit
CCCTGTCTGTGTCTCCAGGGGAGACAGCCACCCTCTCCTGCAGGGCCAGT9250.11755869356070668No Hit
GACAAATTCAGTGGCAGTGGGTCTGGAACAGAGTTCATGCTCACCATCAACAGACTGGAGCCTGAAGATTTTG9190.11679615068355614No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9110.11577942684735543No Hit
GCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGGGAGATTGTGTTGACGCAGTCTC8990.11425434109305437No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8890.1129834362978035No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8850.11247507437970314No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8830.11222089342065296No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8710.1106958076663519No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8690.11044162670730173No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8660.11006035526872646No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8620.10955199335062611No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8610.10942490287110102No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT8600.10929781239157593No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8510.10815399807585013No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8450.10739145519869961No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8340.10599345992392363No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8240.10472255512867276No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8200.1042141932105724No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7900.10040147882481976No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAC200.007733144351.1034330
GGTATCA8300.046.1764641
CGGACTG350.001197910340.38375553
AATGGTA3600.039.12700370
GTATCAA21700.037.8247451
TATCAAC23050.034.7044532
TCGCTGC400.002837849533.8784313
GATTCAC2150.033.1284561
ATCAACG24750.032.992893
TCAACGC24650.032.851824
CAACGCA25150.032.196665
AACGCAG25400.031.7463746
GGGACGT553.6021293E-430.82796120
ACGCAGA26900.029.9761317
AACTGAA4950.029.81104970
GACACGT3200.029.34525170
CGCAGAG27550.029.2688928
AGAGTAC27850.029.0752611
GACAGGT1750.029.071911
GCAGAGT28250.028.5436429
Produced by FastQC (version 0.11.5)