FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1291591
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATCGTACTTCACCGTGGACGTTCGGCCAAGGGA55880.43264469944432876No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40740.315424929408768No Hit
CAGTAATACACTGCAAAATCTTCAGGTTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCTGACCCACTGCCA37370.28933307835065436No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35790.27710010367058924No Hit
ACATATGCCTGGCCAGGCTCCCAGGCTCCTCATCCACAGTGTGTCCAAGAGGGCCGCTGGCACCCCCGACAGGTT31360.24280132023217876No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30680.23753649568632795No Hit
GTATCGTACTTCACCGTGGACGTTCGGCCAAGGGACCAAGGTTGAGATCAGACGAACTGTGGCTGCACCAT27820.21539326303760248No Hit
GTACGATACTGCTGACAGTAATACACTGCAAAATCTTCAGGTTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC26620.20610239619198337No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26430.20463134227476035No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26330.20385710337095875No Hit
GTGTATTACTGTCAGCAGTATCGTACTTCACCGTGGACGTTCGGCCAAGGGACCAAGGTTGAGATCAGACGAA26160.20254089723449603No Hit
CAGTAATACATGGCCGTGTCCGCAGCGGTCACAGAGTGCAAGTTCAGGGCGAACTGGTTCTGGGACGTGTCTA25000.1935597259503976No Hit
GTAATACATGGCCGTGTCCGCAGCGGTCACAGAGTGCAAGTTCAGGGCGA23990.18573991302200155No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22150.1714939171920523No Hit
CTCTTGCAGGGCCAGTGAGAGTGTTAGTAATTACTACTTAGCCTGGTACC21820.16893892880950703No Hit
GTAATACACTGCAAAATCTTCAGGTTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCTGACCCACTGCCA20890.16173850700415224No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20870.16158365922339193No Hit
GTCTCCAGGCACCCTGTCTTTGGCTCCAGGGGAAAGAGCCACCCTCTCTT20560.15918351862160698No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20450.15833185582742523No Hit
GTCTTTGGCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGTGAGA20190.15631883467754112No Hit
GTATTACTGTGTGAGAGTTGCCGGTCTGACTCGCCTCTACTTTGACTCCTGGGGCCTGGGAACCCTGGTCACCGT19980.15469293297955777No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19790.15322187906233475No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19710.15260248793929349No Hit
GTCCAAGAGGGCCGCTGGCACCCCCGACAGGTTCAGTGGCAGTGGGTCAGGGACAGACTTCACTCTCACCATCAG19140.14818932618762443No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18900.1463311528185006No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18380.14230511051873232No Hit
CTCCAGGCACCCTGTCTTTGGCTCCAGGGGAAAGAGCCACCCTCTCTTGC18340.14199541495721169No Hit
GGCATATGTTGGTACCAGGCTAAGTAGTAATTACTAACACTCTCACTGGCCCTGCAAGAGAGGGTGGCTCTTTCC18160.14060178493036882No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17920.138743611561245No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17650.13665316652098072No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17170.13293681978273308No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16970.13138834197512989No Hit
TCCACAGTGTGTCCAAGAGGGCCGCTGGCACCCCCGACAGGTTCAGTGGCAGTGGGTCAGGGACAGACTTCACT16960.13131091808474973No Hit
CATCCACAGTGTGTCCAAGAGGGCCGCTGGCACCCCCGACAGGTTCAGTGGCAGTGGGTCAGGGACAGACTTCA16570.12829138635992354No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC16480.12759457134650212No Hit
ACCTACTACAGCCCCTCCTTCAAGAGTCGCCTCACCATCTCAGTAGACACGTCCCAGAACCAGTTCGCCCTGAA16360.12666548466194022No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCAACCTTGGTCC16220.12558155019661799No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16170.12519443074471717No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15930.12333625737559337No Hit
ATGTATTACTGTGTGAGAGTTGCCGGTCTGACTCGCCTCTACTTTGACTC15810.12240717069103145No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC15610.12085869288342826No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT15570.12054899732190764No Hit
GTAGTAGGTGGTCCCACTGTAATAGATATGCCCGATCCACTCCAGTCTCTTCCCTGGGGGCTGCCGGATCCAGCC15250.11807143282974256No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14930.11559386833757745No Hit
GTGAAGTACGATACTGCTGACAGTAATACACTGCAAAATCTTCAGGTTCCAGTCTGCTGATGGTGAGAGTGAAGT14790.11450993387225523No Hit
CCTCATCCACAGTGTGTCCAAGAGGGCCGCTGGCACCCCCGACAGGTTCAGTGGCAGTGGGTCAGGGACAGACTT14790.11450993387225523No Hit
GTGTCCAAGAGGGCCGCTGGCACCCCCGACAGGTTCAGTGGCAGTGGGTC14390.11141297825704886No Hit
GTGCAGCCACAGTTCGTCTGATCTCAACCTTGGTCCCTTGGCCGAACGTC13990.1083160226418425No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13840.10715466428614011No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13740.10638042538233852No Hit
GTACCAACATATGCCTGGCCAGGCTCCCAGGCTCCTCATCCACAGTGTGTCCAAGAGGGCCGCTGGCACCCCCGA13700.1060707298208179No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13670.10583845814967742No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13640.10560618647853695No Hit
GACTTCACTCTCACCATCAGCAGACTGGAACCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATCGTACTTCA13430.10398028478055359No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13420.10390286089017343No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACGCAGTCTC13420.10390286089017343No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG13370.10351574143827263No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13260.1026640786440909No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT13250.10258665475371073No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13190.10212211141142978No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12990.1005736336038266No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12970.10041878582306629No Hit
GTAATAGATATGCCCGATCCACTCCAGTCTCTTCCCTGGGGGCTGCCGGA12950.10026393804230596No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)