FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1291591
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATCGTACTTCACCGTGGACGTTCGGCCAAGGGA52160.4038430122229096No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41160.31867673280473463No Hit
CAGTAATACACTGCAAAATCTTCAGGTTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCTGACCCACTGCCA33960.26293153173102013No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30070.23281363837313823No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28490.2205806636930731No Hit
ACATATGCCTGGCCAGGCTCCCAGGCTCCTCATCCACAGTGTGTCCAAGAGGGCCGCTGGCACCCCCGACAGGTT27710.21454160024342073No Hit
GTACGATACTGCTGACAGTAATACACTGCAAAATCTTCAGGTTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC27640.2139996330107596No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27270.2111349490666937No Hit
GTATCGTACTTCACCGTGGACGTTCGGCCAAGGGACCAAGGTTGAGATCAGACGAACTGTGGCTGCACCAT26360.20408937504209926No Hit
GTGTATTACTGTCAGCAGTATCGTACTTCACCGTGGACGTTCGGCCAAGGGACCAAGGTTGAGATCAGACGAA24890.19270806315621586No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23370.18093963181843167No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23230.17985569735310947No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22880.1771458611898039No Hit
GTAATACATGGCCGTGTCCGCAGCGGTCACAGAGTGCAAGTTCAGGGCGA22650.17536511171106023No Hit
CAGTAATACATGGCCGTGTCCGCAGCGGTCACAGAGTGCAAGTTCAGGGCGAACTGGTTCTGGGACGTGTCTA22260.17234557998623404No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21700.16800984212494513No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20490.1586415513889459No Hit
CTCTTGCAGGGCCAGTGAGAGTGTTAGTAATTACTACTTAGCCTGGTACC20470.15848670360818556No Hit
GTATTACTGTGTGAGAGTTGCCGGTCTGACTCGCCTCTACTTTGACTCCTGGGGCCTGGGAACCCTGGTCACCGT20310.15724792136210303No Hit
GTCTCCAGGCACCCTGTCTTTGGCTCCAGGGGAAAGAGCCACCCTCTCTT19810.15337672684309508No Hit
GTAATACACTGCAAAATCTTCAGGTTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCTGACCCACTGCCA19730.1527573357200538No Hit
GTCCAAGAGGGCCGCTGGCACCCCCGACAGGTTCAGTGGCAGTGGGTCAGGGACAGACTTCACTCTCACCATCAG19290.1493506845433268No Hit
GTCTTTGGCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGTGAGA18430.1426922299706331No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17860.13827906821896405No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17610.13634347095946006No Hit
ACCTACTACAGCCCCTCCTTCAAGAGTCGCCTCACCATCTCAGTAGACACGTCCCAGAACCAGTTCGCCCTGAA17540.13580150372679897No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17430.13494984093261722No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17110.13247227644045212No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17110.13247227644045212No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16930.13107864641360925No Hit
CTCCAGGCACCCTGTCTTTGGCTCCAGGGGAAAGAGCCACCCTCTCTTGC16770.12983986416752671No Hit
GGCATATGTTGGTACCAGGCTAAGTAGTAATTACTAACACTCTCACTGGCCCTGCAAGAGAGGGTGGCTCTTTCC16600.12852365803106403No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC16580.1283688102503037No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC16580.1283688102503037No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16280.12604609353889892No Hit
CATCCACAGTGTGTCCAAGAGGGCCGCTGGCACCCCCGACAGGTTCAGTGGCAGTGGGTCAGGGACAGACTTCA15850.12271686625255208No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCAACCTTGGTCC15810.12240717069103145No Hit
ATGTATTACTGTGTGAGAGTTGCCGGTCTGACTCGCCTCTACTTTGACTC15710.12163293178722985No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15670.12132323622570923No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT15570.12054899732190764No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15380.11907794340468461No Hit
TCCACAGTGTGTCCAAGAGGGCCGCTGGCACCCCCGACAGGTTCAGTGGCAGTGGGTCAGGGACAGACTTCACT15050.11652295502213937No Hit
GTGCAGCCACAGTTCGTCTGATCTCAACCTTGGTCCCTTGGCCGAACGTC14960.11582614000871794No Hit
GTGTCCAAGAGGGCCGCTGGCACCCCCGACAGGTTCAGTGGCAGTGGGTC14950.11574871611833776No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14640.11334857551655285No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14620.11319372773579252No Hit
GTGAAGTACGATACTGCTGACAGTAATACACTGCAAAATCTTCAGGTTCCAGTCTGCTGATGGTGAGAGTGAAGT14600.1130388799550322No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14220.11009677212058615No Hit
GTAGTAGGTGGTCCCACTGTAATAGATATGCCCGATCCACTCCAGTCTCTTCCCTGGGGGCTGCCGGATCCAGCC14030.10862571820336314No Hit
GACTTCACTCTCACCATCAGCAGACTGGAACCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATCGTACTTCA13980.10823859875146234No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13800.10684496872461949No Hit
CCTCATCCACAGTGTGTCCAAGAGGGCCGCTGGCACCCCCGACAGGTTCAGTGGCAGTGGGTCAGGGACAGACTT13580.10514164313625597No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13460.10421255645169407No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13380.10359316532865281No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12970.10041878582306629No Hit
GTACCAACATATGCCTGGCCAGGCTCCCAGGCTCCTCATCCACAGTGTGTCCAAGAGGGCCGCTGGCACCCCCGA12920.10003166637116549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT903.9910447E-851.81429770
GGTATCA13500.042.6285971
CTAACGC350.001439962438.9019973
GTATCAA30100.032.1292081
GGTCTAG652.5384714E-531.4330161
AATGGTA7650.030.47899670
TATCAAC32750.029.4186232
CACGATT500.00631600328.76993863
TCAACGC33500.028.7522014
ATCAACG33900.028.6170653
GGTACGT1104.085923E-728.25609668
CAACGCA34400.028.1978445
AACGCAG34550.028.1739316
TGTTAGA9700.028.15828370
AACTGTG26800.028.08909270
CGACTGT2600.027.50175919
TAGACTA500.00826437127.2282335
CCGACTG2700.026.48317518
ACGCAGA36900.026.4718957
CGCAGAG37350.026.3352078
Produced by FastQC (version 0.11.5)