FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280510
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36820.28754168261083474No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT35600.27801422870575004No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT34480.2692677136453444No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34190.2670029909957751No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC34110.26637823992003185No Hit
GATCTATGGTGTATCCAATTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGGCAGAATTCA31180.24349673177093503No Hit
GTCTGGGACTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGTTTCTGGAGGCGCCTTCAGCAT30210.235921624977548No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT30060.2347502167105294No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG29470.23014267752692286No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG28130.2196780970082233No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26880.20991636144973486No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA24990.1951566172853004No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24740.1932042701736027No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24360.19023670256382222No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23820.18601963280255523No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG23280.18180256304128822No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22500.17571124005279146No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21870.1707913253313133No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT21660.16915135375748727No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20460.15978008762133836No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19580.15290782578816253No Hit
AGTCAGGGCAGTACCAATAATTTAGCCTGGTTTCAGCAGAAACCAGGTAC19350.15111166644540067No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG19340.15103357256093275No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19140.1494716948715746No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18330.14314609022967412No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18260.14259943303839875No Hit
GTCCCTAAGCGCCTGATCTATGGTGTATCCAATTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT17830.13924139600627874No Hit
GCATTATGATTACCCTCTGAGGTGGACGTTCGGCCAAGGGACCAAGGTGG17810.13908520823734294No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17750.1386166449305355No Hit
CCATAGATCAGGCGCTTAGGGACTGTACCTGGTTTCTGCTGAAACCAGGC17370.135649077320755No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17230.13455576293820432No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16800.13119772590608428No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16420.1282301582963038No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16200.12651209283800985No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15890.12409118241950472No Hit
ACTTATTACTGTCTACAGCATTATGATTACCCTCTGAGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA15060.11760939000866842No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC14840.11589132455037446No Hit
GTCTACAGCATTATGATTACCCTCTGAGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG14700.11479801016782375No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14690.11471991628335584No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14610.1140951652076126No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14130.11034665875315304No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14090.11003428321528141No Hit
TCATAATGCTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTCAGAGTGAATTCTGCC13900.10855049941039117No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC13680.10683243395209721No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA13560.10589530733848232No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13550.10581721345401443No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13460.10511436849380326No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC13250.1034743969199772No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA13220.10324011526657348No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13130.10253727030636231No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGTTCTGG13010.10160014369274742No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCG250.007557480651.40242869
GGTATCA17050.045.566251
TCGTATG6700.041.22516340
CGTATGC6850.039.8992841
ATCTCGT6900.038.8104137
ATGCCGT7150.037.93017244
ACGAGAC8000.037.32584822
TATACAC8100.037.2693943
CTTATAC7950.037.1265261
TCTCCGA8050.036.6624611
CGAGACA8200.036.42115423
TGCCGTC7400.036.23110245
GTATCAA38400.036.2230341
GATCTCG7500.036.1176636
GTATGCC7600.036.0364942
CACGAGA8200.035.99743321
TGGTATC3900.035.6587942
ATCTCCG8300.035.5581710
CCCACGA8300.035.5581719
GTGGTAT4050.035.182481
Produced by FastQC (version 0.11.5)