FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280510
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74720.5835175047442034No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT36560.2855112416146692No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33580.2622392640432328No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT32830.25638222270813976No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC31230.24388720119327456No Hit
GTCTGGGACTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGTTTCTGGAGGCGCCTTCAGCAT30490.2381082537426494No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30460.23787397208924568No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG27890.21780384378099352No Hit
GATCTATGGTGTATCCAATTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGGCAGAATTCA27790.21702290493631443No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26790.2092135164895237No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG25860.20195078523400833No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA25580.1997641564689069No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT24820.19382902124934598No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23870.18641010222489476No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23180.18102162419660917No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23010.17969402816065475No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22340.17446173790130495No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19780.15446970347752068No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG19700.15384495240177742No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18590.1451765312258397No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18240.14244324526946295No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17990.14049089815776525No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17620.1376014244324527No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17490.13658620393436988No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG17020.13291579136437826No Hit
AGTCAGGGCAGTACCAATAATTTAGCCTGGTTTCAGCAGAAACCAGGTAC16990.13268150971097453No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16990.13268150971097453No Hit
GCATTATGATTACCCTCTGAGGTGGACGTTCGGCCAAGGGACCAAGGTGG16920.13213485251969917No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16410.1281520644118359No Hit
CCATAGATCAGGCGCTTAGGGACTGTACCTGGTTTCTGCTGAAACCAGGC16360.12776159498949635No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA16280.12713684391375313No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16280.12713684391375313No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16100.1257311539933308No Hit
GTCCCTAAGCGCCTGATCTATGGTGTATCCAATTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT16030.12518449680205543No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15030.1173751083552647No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14840.11589132455037446No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14810.11565704289697074No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14470.1130018508250619No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT14470.1130018508250619No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14200.11089331594442839No Hit
GTCTACAGCATTATGATTACCCTCTGAGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG14090.11003428321528141No Hit
ACTTATTACTGTCTACAGCATTATGATTACCCTCTGAGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA14020.10948762602400607No Hit
TCATAATGCTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTCAGAGTGAATTCTGCC13780.10761337279677628No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA13770.10753527891230838No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13750.10737909114337256No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13450.10503627460933533No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13380.10448961741805998No Hit
GCCTCATGCATCACGGAGCATGAGAAGACGTTCCCCTGCTGCCACCTGCT13310.10394296022678465No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13260.10355249080444509No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13230.10331820915104137No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13140.10261536419083023No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA13040.10183442534615114No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12990.10144395592381161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14450.043.149861
CTAACGC651.3440513E-841.670893
GTATCAA35700.038.917511
GTGGTAT3100.037.1660731
CCTAACG808.3471605E-833.868182
TATCAAC42850.032.0898062
ATCAACG43350.031.5535623
TCAACGC43550.031.4086554
CAACGCA44800.030.5311035
AACGCAG44750.030.4895616
GCGAGTG450.0050699230.0932949
AGTGGTG8850.029.839435
CACTCGT1451.4551915E-1129.59903358
TGGTATC4150.029.3796272
CGCAGAG47550.028.764258
AGAGTAC49050.028.16069611
TGTTAGA11150.028.13250770
CAGAGTA49700.027.79239710
TCGTATA1454.1472958E-1027.39006661
GCGCCTT9700.027.35298763
Produced by FastQC (version 0.11.5)