FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065088
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86100.41693138500635324No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48990.23722959990082745No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA39110.18938660241113212No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38100.18449576967180092No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37250.1803797223169182No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32090.15539289366845382No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC31120.15069575727523477No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29870.14464274645923078No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29590.14328687203644588No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28370.13737913348002603No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27450.13292411751944713No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA26580.12871122199150836No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25960.12570892862677038No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24530.11878428425326185No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG24390.1181063470418694No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24250.11742840983047695No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24000.11621780766727617No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22910.1109395822357207No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22720.11001952459168811No Hit
GGCCAAAAGTGTACCAGTGTGTCCCTGCCATGCAGTAATAAATCCCAACA22620.10953528372640779No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22300.10798571295751076No Hit
GTGTACCAGTGTGTCCCTGCCATGCAGTAATAAATCCCAACATCCTCAGC21890.10600032540986147No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21170.10251379117984319No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20970.10154530944928254No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAAGCGGGAC20830.10086737223789011No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20800.100722099978306No Hit
CTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCC20680.10014101093996962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20550.041.265891
GTATCAA45250.034.847051
GTGGTAT6900.033.0696331
TATCAAC51900.030.1020762
TCAACGC51900.030.0299474
ATCAACG52100.029.848633
CAACGCA52500.029.6867495
AACGCAG53900.029.1057726
CGCAGAG58500.026.8752848
AACGTTA950.00908049226.70715570
AGAGTAC59000.026.70326611
TACGTAC1307.3159754E-926.17405712
TGGTATC8600.026.1214082
ATACGTA1058.6706495E-725.92603311
CAGAGTA60800.025.80140110
GCAGAGT61100.025.7310339
TGTTAGA14250.025.37179870
ACGCAGA62500.025.1008177
ACGGGTA2550.024.02843716
TTCGGGG13750.023.9878870
Produced by FastQC (version 0.11.5)