FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1209924
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA35250.2913406131294197No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG31480.26018163124295407No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT30630.253156396600117No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCGGTTTATGATATACACTGGTATCAGCAACTTCCAGGGACAGC28140.23257659158757077No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC27530.2275349526085936No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG27150.2243942594741488No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC24540.20282265662967264No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA23140.19125168192382333No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23120.19108638228516833No Hit
GTAATAAAAGGCCGTGTCTGCGGCGGTCACAGAACTCAGCTTCAGGGAGA21510.17777976137344165No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCT21130.17463906823899683No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT21010.1736472704070669No Hit
GTCTCTGGTTCCTCCATCAGCAGTGCTTACTACTGGGGCTGGATCCGGCA20680.17091982636925956No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC20140.16645673612557482No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC19640.16232424515920008No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT19520.16133244732727015No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCTGAGCACTCAGG19250.1591009022054278No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCTGAGCACTCAGGCTGTTGTCATAGGA19230.15893560256677278No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG19060.15753055563820537No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC19000.1570346567222404No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18500.15290216575586565No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC18420.1522409672012457No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC18030.1490176242474734No Hit
GATTATTACTGCCAGTCCTATGACAACAGCCTGAGTGCTCAGGTCTTCGG17890.14786052677688846No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT17760.14678607912563105No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT17710.14637283002899357No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17490.14455453400378868No Hit
GTTTATGATATACACTGGTATCAGCAACTTCCAGGGACAGCCCCCAAGCTCCTCATCTATGGTAACAACAATCGG17470.14438923436513368No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCTGAGCACTCAGGC17400.14381068562984123No Hit
GCTTACTACTGGGGCTGGATCCGGCAGCCCCCAGGGAAGGGTCTGGAGTG17080.14116589141136138No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16930.13992614412144896No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG16560.13686810080633163No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15460.12777662068030718No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT15330.12670217302904976No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC15130.12504917664249987No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCGGTTTATGATATACACTGGTATCAG14970.12372677953325995No Hit
GTCTATCATAAGGGAAATACCTACCACAATCCGTCCCTCAAGAGTCGAGT14840.1226523318820025No Hit
GGTCTGGAGTGGATTGGGTCTGTCTATCATAAGGGAAATACCTACCACAA14740.12182583368872756No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT14640.1209993354954526No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC14520.12000753766352265No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT14370.11876779037361025No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT14090.11645359543244038No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT14020.11587504669714792No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG13870.1146352994072355No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACAACAGCCTGAGTGCTCAGGTCTTCGGAACTGGGACCAAGGT13770.11380880121396054No Hit
GATATACACTGGTATCAGCAACTTCCAGGGACAGCCCCCAAGCTCCTCAT13650.11281700338203061No Hit
GTGATGGCCAGGGAGGCTGAGGAGCCAGACTTGGAGCCAGAGAATCGGTC13410.11083340771817073No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC13410.11083340771817073No Hit
ATGATATACACTGGTATCAGCAACTTCCAGGGACAGCCCCCAAGCTCCTCATCTATGGTAACAACAATCGGCCCT13380.11058545826018824No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG13380.11058545826018824No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC13100.10827126331901839No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT13040.10777536440305341No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG13020.10761006476439842No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC13000.10744476512574344No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12910.10670091675179598No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAACAG12870.10637031747448598No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT12690.10488262072659109No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC12440.1028163752434037No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG12410.10256842578542123No Hit
CCTGTGAGGTGGCTGCGTACTTGCCCCCTCTCAGGACTGATGGGAAGCCC12290.1015766279534913No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC12110.10008893120559638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3300.073.2719770
TTCCGTG200.00777434451.036487
CGTATGC4050.042.6540141
ACGAGAC4250.042.4303322
TCGTATG4000.042.259640
GCCGTCT4250.041.75406346
GTATGCC4200.041.18942342
CTTATAC4400.040.2221951
TCTCGTA3650.039.58146738
AATCTCG3800.038.81722336
CACGAGA4650.038.78040721
TTATACA4700.038.3741952
CCGTCTT4650.038.2059147
ATGCCGT4550.038.1267544
GTATCAA22250.037.9347081
CGAGACA4850.037.18582523
GCTTGAA4800.036.89505456
TGCCGTC4850.036.5345745
ACACATC4850.036.4857146
TGCTTGA4850.036.43162555
Produced by FastQC (version 0.11.5)