FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1209924
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42150.3483689884653912No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG35200.29092736403278224No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC30940.2557185409992694No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA28300.23389898869681072No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT27740.229270598814471No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCACTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG26400.21819552302458667No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCGGTTTATGATATACACTGGTATCAGCAACTTCCAGGGACAGC26370.21794757356660416No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA25980.21472423061283186No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC24450.20207880825572513No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG23360.19306997794902822No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCT22500.18596209348686366No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC21010.1736472704070669No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20980.1733993209490844No Hit
GTAATAAAAGGCCGTGTCTGCGGCGGTCACAGAACTCAGCTTCAGGGAGA20750.17149837510455201No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC19020.1571999563608954No Hit
GTCTCTGGTTCCTCCATCAGCAGTGCTTACTACTGGGGCTGGATCCGGCA18970.15678670726425792No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC18820.1555469599743455No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18230.15067062063402328No Hit
GTTTATGATATACACTGGTATCAGCAACTTCCAGGGACAGCCCCCAAGCTCCTCATCTATGGTAACAACAATCGG18090.14951352316343836No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT18040.14910027406680088No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17930.14819112605419846No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCTGAGCACTCAGGCTGTTGTCATAGGA17060.14100059177270638No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC16950.14009144376010393No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16890.13959554484413897No Hit
GCTTACTACTGGGGCTGGATCCGGCAGCCCCCAGGGAAGGGTCTGGAGTG16670.13777724881893408No Hit
GATTATTACTGCCAGTCCTATGACAACAGCCTGAGTGCTCAGGTCTTCGG16390.13546305387776422No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC16360.13521510441978174No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG16330.13496715496179926No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC16160.13356210803323185No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT15810.1306693643567695No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT15660.1294296170668571No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC15590.12885106833156462No Hit
GGTCTGGAGTGGATTGGGTCTGTCTATCATAAGGGAAATACCTACCACAA15180.12546242573913735No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCTGAGCACTCAGG15060.12447062790720739No Hit
GTCTATCATAAGGGAAATACCTACCACAATCCGTCCCTCAAGAGTCGAGT14800.12232173260469253No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT14770.12207378314671004No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG14630.12091668567612511No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT14540.12017283730217766No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCGGTTTATGATATACACTGGTATCAG14470.1195942885668852No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC14380.11885044019293774No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT14270.11794129218033529No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCACTGTCTGGGGCCCCAGGGCAGAGGGTCAC14110.11661889507109538No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC14080.1163709456131129No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCTGAGCACTCAGGC14000.11570974705849292No Hit
GTGCTGACGCAGCCGCCCTCACTGTCTGGGGCCCCAGGGCAGAGGGTCAC14000.11570974705849292No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC13820.11422205031059803No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACAACAGCCTGAGTGCTCAGGTCTTCGGAACTGGGACCAAGGT13690.11314760265934058No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT13270.1096763102475858No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG13230.10934571097027582No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT13190.10901511169296584No Hit
GATATACACTGGTATCAGCAACTTCCAGGGACAGCCCCCAAGCTCCTCAT13170.10884981205431085No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13100.10827126331901839No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG13030.10769271458372592No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCACTGTCT12950.10703151602910596No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12680.10479997090726359No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT12630.10438672181062614No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC12620.10430407199129862No Hit
ATGATATACACTGGTATCAGCAACTTCCAGGGACAGCCCCCAAGCTCCTCATCTATGGTAACAACAATCGGCCCT12450.10289902506273121No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT12270.10141132831483632No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)