FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215933
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36730.3020725648534911No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34220.2814299801058118No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26980.22188722569417887No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA25210.2073305025852576No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23800.19573446892221855No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23020.18931964178947358No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22730.18693464195806841No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22360.18389171113868938No Hit
GTGTAAACTGGAAGCAGCATAGATCAGGAGTTTGGGGGCTTTCCCTGGTC21770.17903947010238228No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21730.17871050460839538No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20990.1726246429696373No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20880.17171998786117326No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20810.17114429824669616No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20420.16793688468032367No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20380.16760791918633675No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20340.16727895369234982No Hit
ACTTATTACTGTCTACAAGATTACGATTACCCTCTGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAACCGAA20040.16481171248744791No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC19730.16226222990904926No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19150.1574922302462389No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCGCCATCACTT19110.15716326475225195No Hit
CTCCTGATCTATGCTGCTTCCAGTTTACACAGTGGGGTCCCCTCAAGGTT19060.15675205788476834No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18640.15329792019790564No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18340.15083067899300373No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18290.15041947212552007No Hit
GATTTCACTCTCACCGTCAGTAGCCTGCAGCCTGAAGACTTTGCAACTTATTACTGTCTACAAGATTACGATTAC18200.1496792997640495No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18160.14935033427006258No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17890.14712981718565085No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17850.14680085169166393No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17790.14630740345068355No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17050.1402215418119255No Hit
GTATTATTGTGCTAGAGACTCCAGTCCGACGACTTCTTCCTACTTTGACTACTGGGGCCGGGGAACCCTGGTCAC17000.13981033494444184No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16630.1367674041250628No Hit
GTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTACTGACGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCG16530.13594499039009553No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16530.13594499039009553No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16490.13561602489610858No Hit
CAGTTTACACAGTGGGGTCCCCTCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACTCTCACCGTCAGTAG16040.13191516308875573No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16000.13158619759476878No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCCGG15950.13117499072728514No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15830.13018809424532438No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT15800.1299413701248342No Hit
CAATAATACACGGCCGTGTCCTCACTTTTCAGGCTGTTCATCTCCAGATA15650.12870774952238323No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15630.12854326677538977No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTTGACTTCCACCTTGGTCC15560.12796757716091264No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15470.12722740479944208No Hit
GTCTTCAGGCTGCAGGCTACTGACGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCGCTGAACCTTGAGGGGAC15400.12665171518496496No Hit
AGTCAGGGCATTGGAAATGATTTAGGCTGGTATCAGCAGAGACCAGGGAAAGCCCCCAAACTCCTGATCTATG15330.12607602557048783No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15300.12582930144999765No Hit
GATCAGGAGTTTGGGGGCTTTCCCTGGTCTCTGCTGATACCAGCCTAAAT15300.12582930144999765No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15190.12492464634153361No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15040.12369102573908267No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14900.12253964651012844No Hit
GTCTGCATCTGTGGGAGACAGAGTCGCCATCACTTGCCGGGCAAGTCAGG14570.11982568118473634No Hit
GATCTATGCTGCTTCCAGTTTACACAGTGGGGTCCCCTCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCA14440.11875654332927883No Hit
GCCTGAAGACTTTGCAACTTATTACTGTCTACAAGATTACGATTACCCTC14270.11735843997983443No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14250.11719395723284096No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13920.11447999190744886No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13880.11415102641346192No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13730.11291740581101098No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13650.11225947482303714No Hit
GGGTTCCCCGGCCCCAGTAGTCAAAGTAGGAAGAAGTCGTCGGACTGGAG13640.11217723344954039No Hit
GTCTACAAGATTACGATTACCCTCTGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAACCGAACTGTGGCTGCAC13620.11201275070254695No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13610.1119305093290502No Hit
GTGCAGCCACAGTTCGGTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC13480.11086137147359269No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13410.1102856818591156No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT13390.11012119911212213No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGATGACCCAGTCTCCATCCTCC13160.10822964752169734No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13040.10724275103973656No Hit
GTGGGGTCCCCTCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACT12880.10592688906378887No Hit
GTGTAACCTCTGGATTCACCCTCAATGACTACCACATCGACTGGGTCCGC12880.10592688906378887No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGTTATCTTACAAGGTGTCCA12780.10510447532882158No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC12760.10493999258182811No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12710.10452878571434446No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT12690.10436430296735098No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12530.10304844099140331No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12510.10288395824440985No Hit
CCTCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACTCTCACCGTCAGTAGCCTGCAGCCTGAAGACTTTG12280.10099240665398504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2750.041.0771181
GTATCAA24050.035.7254261
GCCGTCT1102.5465852E-1134.77309846
CGTATGC1003.6925485E-1034.60822341
TCGTATG1003.7107384E-1034.57802240
GTATGCC1253.6379788E-1233.25155342
CACGAGA1350.032.9631221
ATCTCGT958.8421075E-932.6671737
ACGAGAC1305.456968E-1231.60038422
AATGGTA2509.094947E-1230.72788470
TATCAAC27850.030.7216282
TAAGACA1359.094947E-1230.52544631
GTTTCGG450.00473885430.51159530
GTTAGCC450.004808099530.4212383
ATCAACG28550.030.0882533
TCAACGC28600.029.9159934
CAACGCA28900.029.6054425
CGCATAA604.7181392E-429.44143159
AACGCAG29200.029.3012776
CAGGTAT1302.1645974E-1028.9646361
Produced by FastQC (version 0.11.5)