FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215933
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36860.3031417027089486No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29420.24195412082738113No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26840.22073584646522462No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26410.21719946740486523No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22250.18298705603022536No Hit
GTGTAAACTGGAAGCAGCATAGATCAGGAGTTTGGGGGCTTTCCCTGGTC22080.18158895268078093No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21960.18060205619882017No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21730.17871050460839538No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21440.1763255047769902No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20920.17204895335516018No Hit
CTCCTGATCTATGCTGCTTCCAGTTTACACAGTGGGGTCCCCTCAAGGTT20530.16884153978878771No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20250.16653878133087924No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19590.16111085068009504No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19130.15732774749924544No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18900.15543619590882063No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18830.15486050629434353No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18750.15420257530636966No Hit
ACTTATTACTGTCTACAAGATTACGATTACCCTCTGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAACCGAA18730.1540380925593762No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18590.15288671333042197No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC18300.1505017134990168No Hit
GATTTCACTCTCACCGTCAGTAGCCTGCAGCCTGAAGACTTTGCAACTTATTACTGTCTACAAGATTACGATTAC17880.1470475758121541No Hit
GTCTTCAGGCTGCAGGCTACTGACGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCGCTGAACCTTGAGGGGAC17600.14474481735424566No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCGCCATCACTT17540.1442513691132653No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17450.1435111967517947No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17430.14334671400480126No Hit
GTATTATTGTGCTAGAGACTCCAGTCCGACGACTTCTTCCTACTTTGACTACTGGGGCCGGGGAACCCTGGTCAC17360.14277102439032413No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17270.14203085202885357No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16800.13816550747450723No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16720.1375075764865334No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16500.1356982662696053No Hit
GTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTACTGACGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCG16410.13495809390813474No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16340.13438240429365764No Hit
GTGGGGTCCCCTCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACT16260.1337244733056838No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16190.13314878369120667No Hit
CAATAATACACGGCCGTGTCCTCACTTTTCAGGCTGTTCATCTCCAGATA16140.132737576823723No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15850.13035257699231784No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15800.1299413701248342No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15350.1262405083174813No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15240.12533585320901727No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15170.12476016359454017No Hit
GTCTGCATCTGTGGGAGACAGAGTCGCCATCACTTGCCGGGCAAGTCAGG15080.12401999123306957No Hit
GTGGGAGACAGAGTCGCCATCACTTGCCGGGCAAGTCAGGGCATTGGAAATGATTTAGGCTGGTATCAGCAGAGA14960.12303309475110882No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14610.12015464667872325No Hit
GGGTTCCCCGGCCCCAGTAGTCAAAGTAGGAAGAAGTCGTCGGACTGGAG14600.12007240530522653No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14480.11908550882326575No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCCGG14340.11793412959431153No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTTGACTTCCACCTTGGTCC14250.11719395723284096No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14230.1170294744858475No Hit
GTCTACAAGATTACGATTACCCTCTGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAACCGAACTGTGGCTGCAC14160.11645378487137037No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14110.11604257800388673No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14100.11596033663038999No Hit
GATCTATGCTGCTTCCAGTTTACACAGTGGGGTCCCCTCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCA14090.11587809525689327No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC14050.11554912976290634No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13990.11505568152192595No Hit
GCCTGAAGACTTTGCAACTTATTACTGTCTACAAGATTACGATTACCCTC13970.1148911987749325No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13830.11373981954597827No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG13820.11365757817248154No Hit
AGTCAGGGCATTGGAAATGATTTAGGCTGGTATCAGCAGAGACCAGGGAAAGCCCCCAAACTCCTGATCTATG13670.11242395757003057No Hit
GATCAGGAGTTTGGGGGCTTTCCCTGGTCTCTGCTGATACCAGCCTAAAT13620.11201275070254695No Hit
CAGTTTACACAGTGGGGTCCCCTCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACTCTCACCGTCAGTAG13570.11160154383506328No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13560.11151930246156654No Hit
GTGCAGCCACAGTTCGGTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC13350.10979223361813521No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13090.10765395790722021No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12860.10576240631679543No Hit
GGGTAATCGTAATCTTGTAGACAGTAATAAGTTGCAAAGTCTTCAGGCTG12830.10551568219630524No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC12680.10428206159385427No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12680.10428206159385427No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12450.10239051000342947No Hit
GAGTACGCCGCGTCTGTTAAAGGCAGATTCACCATCTCAAGAGATAATTC12380.10181482038895236No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12300.1011568894009785No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12270.10091016528048832No Hit
GTGTAACCTCTGGATTCACCCTCAATGACTACCACATCGACTGGGTCCGC12210.10041671703950794No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT12180.10016999291901775No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)